Application of virtual reality in volumetric cellular visualization

Recent advancement in high-resolution confocal imaging has provided valuable novel insights into structural relationships within cells and tissues in vitro and in vivo. Development in volume rendering technique enables visualization of 3D dataset in real-time. Here, we present a system in which 3D models are reconstructed from the stacked 2D images obtained with a confocal microscope, and subsequently visualized using volume rendering technique. Using the reconstructed 3D model, we apply virtual reality method to provide an intuitive way to view and manipulate the 3D cellular environment.

[1]  M Opas,et al.  Ca2+ signaling and calcium binding chaperones of the endoplasmic reticulum. , 2002, Cell calcium.

[2]  T. Todd Elvins,et al.  Visualization of 3D ultrasound data , 1993, IEEE Computer Graphics and Applications.

[3]  Ross T. Whitaker,et al.  Direct visualization of volume data , 1992, IEEE Computer Graphics and Applications.

[4]  Philippe Lacroute,et al.  Analysis of a Parallel Volume Rendering System Based on the Shear-Warp Factorization , 1996, IEEE Trans. Vis. Comput. Graph..

[5]  B.L. Evans,et al.  Knowledge-based visualization of myocardial perfusion tomographic images , 1990, [1990] Proceedings of the First Conference on Visualization in Biomedical Computing.

[6]  J. Pawley,et al.  Handbook of Biological Confocal Microscopy , 1990, Springer US.

[7]  Farrell Ej,et al.  Three-dimensional data visualization and biomedical applications , 1989 .

[8]  E Marth,et al.  Lipoprotein Lp(a) and the risk for myocardial infarction. , 1981, Atherosclerosis.

[9]  M Opas,et al.  Fluorescence tracing of intracellular proteins. , 1999, Biotechnic & histochemistry : official publication of the Biological Stain Commission.

[10]  David L. Becker,et al.  Confocal Microscopy: Methods and Protocols. , 1999 .

[11]  Hanspeter Pfister,et al.  The VolumePro real-time ray-casting system , 1999, SIGGRAPH.

[12]  William E. Lorensen,et al.  Marching cubes: A high resolution 3D surface construction algorithm , 1987, SIGGRAPH.

[13]  M. Frick,et al.  Lp(a) lipoprotein and pre‐β1‐lipoprotein in patients with coronary heart disease , 1974, Clinical genetics.

[14]  Marc Levoy,et al.  Display of surfaces from volume data , 1988, IEEE Computer Graphics and Applications.

[15]  M Opas,et al.  Calreticulin: one protein, one gene, many functions. , 1999, The Biochemical journal.

[16]  Marc Levoy,et al.  Volume rendering in radiation treatment planning , 1990, [1990] Proceedings of the First Conference on Visualization in Biomedical Computing.

[17]  S. Paddock,et al.  Confocal laser scanning microscopy. , 1999, BioTechniques.