Influence of experimental parameters on chiral separation in SFC
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This thesis describes an investigation of the chiral separation of two groups of
molecules by supercritical fluid chromatography (SFC).
The studies were carried out on chiral ChiralCel OB and OD, Cyclobond RN-I and -III and on Pirkle columns using a range of pressure and temperatures from
subcritical liquid carbon dioxide to supercritical conditions with or without the
addition of methanol (or 2-propanol) as a mobile phase modifier. The first study
examined the relationship between structure and resolution for a number of chiral
substituted benzyl alcohols and related compounds. The second study examined the
benzodiazepines, which have been chromatographed by SFC previously in this
laboratory, as examples of fairly polar drugs. [Continues.]
[1] Loren Lyle Braun,et al. Introduction to organic and biochemistry , 1972 .
[2] J. Sevcík. Detectors in gas chromatography , 1976 .