Role of PLLA plasma surface modification in the interaction with human marrow stromal cells

The effects of oxygen-based radio frequency plasma enhanced chemical vapor deposition (rf PECVD) on the surface of poly(L-lactide) (PLLA) polymers and the influence thereof on protein adsorption and on bone–cell behavior have been studied. Thin films and porous scaffolds based on PLLA polymer were developed, and the role of surface modifications were investigated extensively. PECVD surface treatments were used to alter surface functionality and modulate protein adsorption on the PLLA polymer matrix. In particular, Bovine Serum Albumine fluorescein isothiocyanate (fitc-BSA) conjugate adsorption on patterned surfaces of treated PLLA was analyzed by fluorescence microscopy. Human marrow stromal cells (MSCs) were cultured on scaffolds and cell adhesion and morphology were assessed using fluorescence microscopy. The results indicated that the PLLA surface became hydrophilic and its roughness increased with the treatment time and it had a dominant influence on the adsorption process of the protein. The outcome of the plasma treatment of various PLLA surfaces has been shown to be the up-regulator of the cell-adhesive proteins expression and consequently the improvement of cell adhesion and growth. Oxygen-treated PLLA promoted higher adhesion and proliferation of the MSCs in comparison to the untreated samples. It can be concluded that following plasma treatment, PLLA samples show enhanced affinity for osteoprogenitor cells. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009

[1]  C. Satriano,et al.  Improved osteogenic differentiation of human marrow stromal cells cultured on ion-induced chemically structured poly-epsilon-caprolactone. , 2007, Biomaterials.

[2]  J. Six,et al.  Surface characteristics of PLA and PLGA films , 2006 .

[3]  A. Boccaccini,et al.  Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. , 2006, Biomaterials.

[4]  Shen‐guo Wang,et al.  Influences of ammonia plasma treatment on modifying depth and degradation of poly(L-lactide) scaffolds. , 2006, Biomaterials.

[5]  Zhong-yi Jiang,et al.  Improved protein-adsorption-resistant property of PES/SPC blend membrane by adjustment of coagulation bath composition. , 2005, Colloids and surfaces. B, Biointerfaces.

[6]  San-Yuan Chen,et al.  Miscibility, thermal characterization and crystallization of poly(l-lactide) and poly(tetramethylene adipate-co-terephthalate) blend membranes , 2005 .

[7]  C. Satriano,et al.  The effect of irradiation modification and RGD sequence adsorption on the response of human osteoblasts to polycaprolactone. , 2005, Biomaterials.

[8]  Morgan R. Alexander,et al.  Using Plasma Deposits to Promote Cell Population of the Porous Interior of Three‐Dimensional Poly(D,L‐Lactic Acid) Tissue‐Engineering Scaffolds , 2005 .

[9]  In-Seop Lee,et al.  Plasma surface modification of poly (D,L-lactic-co-glycolic acid) (65/35) film for tissue engineering , 2005 .

[10]  Yuqing Wan,et al.  Characterization of surface property of poly(lactide-co-glycolide) after oxygen plasma treatment. , 2004, Biomaterials.

[11]  P. Bongrand,et al.  Is there a predictable relationship between surface physical-chemical properties and cell behaviour at the interface? , 2004, European cells & materials.

[12]  G. Stevens,et al.  Controllable surface modification of poly(lactic-co-glycolic acid) (PLGA) by hydrolysis or aminolysis I: physical, chemical, and theoretical aspects. , 2004, Biomacromolecules.

[13]  Antonios G Mikos,et al.  Biomimetic materials for tissue engineering. , 2003, Biomaterials.

[14]  Yuqing Wan,et al.  Cell adhesion on gaseous plasma modified poly-(L-lactide) surface under shear stress field. , 2003, Biomaterials.

[15]  A S G Curtis,et al.  Fibroblast reaction to island topography: changes in cytoskeleton and morphology with time. , 2003, Biomaterials.

[16]  Jia-cong Shen,et al.  Protein immobilization on the surface of poly-L-lactic acid films for improvement of cellular interactions , 2002 .

[17]  F. Cui,et al.  Culture of neural cells on silicon wafers with nano-scale surface topograph , 2002, Journal of Neuroscience Methods.

[18]  W. Saltzman,et al.  Topographical control of human neutrophil motility on micropatterned materials with various surface chemistry. , 2002, Biomaterials.

[19]  Shen‐guo Wang,et al.  Enhanced cell affinity of poly (D,L-lactide) by combining plasma treatment with collagen anchorage. , 2002, Biomaterials.

[20]  D. Puleo,et al.  A technique to immobilize bioactive proteins, including bone morphogenetic protein-4 (BMP-4), on titanium alloy. , 2002, Biomaterials.

[21]  Antonios G Mikos,et al.  Bioactive poly(L-lactic acid) conduits seeded with Schwann cells for peripheral nerve regeneration. , 2002, Biomaterials.

[22]  Mathis O. Riehle,et al.  The use of materials patterned on a nano- and micro-metric scale in cellular engineering , 2002 .

[23]  J. Vacanti,et al.  In vitro degradation of porous poly(L-lactic acid) foams. , 2000, Biomaterials.

[24]  K. Anselme,et al.  Osteoblast adhesion on biomaterials. , 2000, Biomaterials.

[25]  S. Hsu,et al.  Improved cell adhesion by plasma-induced grafting of L-lactide onto polyurethane surface. , 2000, Biomaterials.

[26]  Robert Langer,et al.  Biodegradable Polymer Scaffolds for Tissue Engineering , 1994, Bio/Technology.

[27]  Robert Langer,et al.  Preparation and characterization of poly(l-lactic acid) foams , 1994 .

[28]  C. M. Alves,et al.  Modulating bone cells response onto starch-based biomaterials by surface plasma treatment and protein adsorption. , 2007, Biomaterials.

[29]  A Giunti,et al.  Poly-epsilon-caprolactone/hydroxyapatite composites for bone regeneration: in vitro characterization and human osteoblast response. , 2006, Journal of biomedical materials research. Part A.

[30]  J. Jansen,et al.  Integrins as linker proteins between osteoblasts and bone replacing materials. A critical review. , 2005, Biomaterials.

[31]  Pietro Favia,et al.  Plasma treatments and plasma deposition of polymers for biomedical applications , 1998 .