A flow cytometric analysis of cytotoxic effects of nitrobenzene on rat spermatogenesis.

Cytotoxic effects of nitrobenzene (NB) on spermatogenesis of mature Sprague-Dawley (Crj:CD) rats were analyzed by measuring the DNA content distribution and testicular weight at 1, 2, and 3 weeks of daily oral dose of NB (60 mg/kg/day). Rats at the age of 9 weeks were used because the ratios of 1C, 2C, S, 4C to total testicular cells were stabilized after the age of 52 days. Within a week of administration, a large number of 1C cells were lost but 2C cells proliferated, resulting in little change of testicular weight. In another week that followed, the number of 1C cells and testicular weight were decreased, but the ratio of S-4C cells was increased, indicating that an appreciable number of 2C cells could progress to the 4C compartment. The data indicated that (1) 1C cells were destroyed, and (2) meiosis of secondary spermatocytes was suppressed, but (3) NB had little effect on the spermatocytes prior to the early pachytene stage. This interpretation was reinforced by the observation that (4) the ratio of 1C cells returned to a nearly normal level during a recovery period of 2 weeks. In conclusion, flow cytometry could offer an efficient method for the quantitative analysis of male reproductive toxicity.

[1]  H. Krishnamurthy,et al.  Evaluation of cytotoxic effects of different doses of vinblastine on mouse spermatogenesis by flow cytometry. , 1996, Toxicology.

[2]  I. Adler.  Comparison of the duration of spermatogenesis between male rodents and humans. , 1996, Mutation research.

[3]  M. Spanô,et al.  Flow cytometric assessment of trophosphamide toxicity on mouse spermatogenesis. , 1996, Cytometry.

[4]  I. Horii,et al.  Flow Cytometric Analysis for the Evaluation of the Rat Sperm Viability and Number in the Male Reproductive Toxicity Studies , 1995 .

[5]  M. Usami,et al.  Studies on the establishment of appropriate spermatogenic endpoints for male fertility disturbance in rodent induced by drugs and chemicals. I. Nitrobenzene. , 1995, The Journal of toxicological sciences.

[6]  I. Dobrinski,et al.  Flow cytometric and microscopic evaluation and effect on fertility of abnormal chromatin condensation in bovine sperm nuclei. , 1994, Journal of reproduction and fertility.

[7]  G. Mikuz,et al.  Quantification of spermatogenesis by dual-parameter flow cytometry. , 1994, Fertility and sterility.

[8]  M. Meistrich,et al.  Hormonal protection from procarbazine-induced testicular damage is selective for survival and recovery of stem spermatogonia. , 1994, Cancer research.

[9]  P. McCue,et al.  Validation of flow cytometry analysis in the objective assessment of spermatogenesis: comparison to the quantitative testicular biopsy. , 1993, The Journal of urology.

[10]  D. Ofner,et al.  Deoxyribonucleic acid flow cytometry and semiquantitative histology of spermatogenesis: a comparative study. , 1992, Fertility and sterility.

[11]  L. Strader,et al.  Endpoints of spermatotoxicity in the rat after short duration exposures to fourteen reproductive toxicants. , 1992, Reproductive toxicology.

[12]  B. Kirkhus,et al.  BrdUrd incorporation studies for evaluation of spermatogenesis in the blue fox. , 1992, Cytometry.

[13]  R. Sharpe,et al.  Changes in Sertoli cell function in vitro induced by nitrobenzene. , 1990, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[14]  R. Baer,et al.  Flow cytometric analysis of effects of 1,3-dinitrobenzene on rat spermatogenesis. , 1989, Journal of toxicology and environmental health.

[15]  A. Levin,et al.  The reversibility of nitrobenzene-induced testicular toxicity: continuous monitoring of sperm output from vasocystotomized rats. , 1988, Toxicology.

[16]  L. Strader,et al.  Acute effects and long-term sequelae of 1,3-dinitrobenzene on male reproduction in the rat. II. Quantitative and qualitative histopathology of the testis. , 1988, Journal of andrology.

[17]  D. Evenson,et al.  Mouse testicular and sperm cell development characterized from birth to adulthood by dual parameter flow cytometry. , 1986, Biology of reproduction.

[18]  S. Kawai.  [DNA flow cytometric evaluation of spermatogenesis. Part 1: Analysis of nuclear DNA in cells from the human testicular tissue]. , 1984, Hinyokika kiyo. Acta urologica Japonica.

[19]  M. Melamed,et al.  Rapid Analysis of Normal and Abnormal Cell Types in Human Semen and Testis Biopsies by Flow Cytometry 1 2 , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[20]  R. Irons,et al.  A critical review of the literature on nitrobenzene toxicity. , 1982, Critical reviews in toxicology.

[21]  J. A. Bond,et al.  Induction of hepatic and testicular lesions in Fischer-344 rats by single oral doses of nitrobenzene. , 1981, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[22]  O. Clausen,et al.  Application of micro-flow fluorometry to studies of meiosis in the male rat. , 1977, Biology of reproduction.

[23]  Y. Clermont,et al.  DURATION OF THE CYCLE OF THE SEMINIFEROUS EPITHELIUM OF NORMAL, HYPOPHYSECTOMIZED AND HYPOPHYSECTOMIZED-HORMONE TREATED ALBINO RATS. , 1965, Endocrinology.

[24]  C. P. Leblond,et al.  DEFINITION OF THE STAGES OF THE CYCLE OF THE SEMINIFEROUS EPITHELIUM IN THE RAT , 1952, Annals of the New York Academy of Sciences.