Cell counting of body fluids: comparison between three automated haematology analysers and the manual microscope method

Haematological analysis of body fluids (BF) specimens can provide clinicians with valuable diagnostic information because it can indicate one of several serious medical conditions. Although up to now the microscopic counting and the differentiation of WBC in a BF smear have been used as a reference. The introduction of semiautomated and automated methods of analysis has reduced interoperator variability and improved turnaround time and precision. The aim of our study was to evaluate the accuracy and the correlation between the three methods and with the reference method.

[1]  P. Sinha,et al.  Leukocyte counts in cerebrospinal fluid with the automated hematology analyzer CellDyn 3500 and the urine flow cytometer UF-100. , 2000, Clinical chemistry.

[2]  S. Sandberg,et al.  Automated counting of white and red blood cells in the cerebrospinal fluid. , 2000, Clinical and laboratory haematology.

[3]  A. S. Cole,et al.  [Body fluids]. , 1987, Nihon rinsho. Japanese journal of clinical medicine.

[4]  A. Verstraete,et al.  Automated flow cytometric analysis of cerebrospinal fluid. , 2001, Clinical chemistry.

[5]  Terri D. Richmond,et al.  Performance evaluation of the application of body fluids on the Sysmex XE-2100 series automated hematology analyzer. , 2005, Laboratory hematology : official publication of the International Society for Laboratory Hematology.

[6]  J. Machan,et al.  The clinical reliability of automated cerebrospinal fluid cell counts on the Beckman-Coulter LH750 and Iris iQ200. , 2009, American journal of clinical pathology.

[7]  K. Lackner,et al.  Automated flow cytometric analysis of blood cells in cerebrospinal fluid: analytic performance. , 2004, American Journal of Clinical Pathology.

[8]  I. Talstad.  Electronic counting of spinal fluid cells. , 1984, American journal of clinical pathology.

[9]  H. Schumacher,et al.  A Practical Handbook of Joint Fluid Analysis , 1984 .

[10]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.

[11]  M. Keeney,et al.  Validation of body fluid analysis on the Coulter LH 750. , 2003, Laboratory hematology : official publication of the International Society for Laboratory Hematology.

[12]  A. Kratz,et al.  The ADVIA 2120 hematology system: flow cytometry-based analysis of blood and body fluids in the routine hematology laboratory. , 2005, Laboratory hematology : official publication of the International Society for Laboratory Hematology.

[13]  C L Rümke,et al.  Imprecision of ratio-derived differential leukocyte counts. , 1985, Blood cells.