Miniaturized disposable biosensors

Abstract Miniaturized amperometric thick film biosensors for applications in food quality control are described. Their ease of fabrication and relatively low cost facilitates their use as disposable sensors. As an alternative to the immobilization of the enzyme by cross-linking with glutaraldehyde, screen printing technology was used to apply the enzyme layer, a graphite/TTF based paste, to the thick film electrodes. Examples are presented of the determination of biogenic amines as an indicator of the freshness of fish, and of glucose in fruit juice and wine.