Prediction of fat quality in pig carcasses by near-infrared spectroscopy.

This study was conducted to evaluate the potential of near-infrared (NIR) spectroscopy (NIRS) technology for prediction of the chemical composition (moisture content and fatty acid composition) of fat from fast-growing, lean slaughter pig samples coming from breeding programmes. NIRS method I: a total of 77 samples of intact subcutaneous fat from pigs were analysed with the FOSS FoodScan NIR spectrophotometer (850 to 1050 nm) and then used to predict the moisture content by using partial least squares (PLS) regression methods. The best equation obtained has a coefficient of determination for cross-validation (CV; R(2)(cv)) and a root mean square error of a CV (RMSECV) of 0.88 and 1.18%, respectively. The equation was further validated with (n = 15) providing values of 0.83 and 0.42% for the coefficient of determination for validation (R(2)(val)) and root mean square error of prediction (RMSEP), respectively. NIRS method II: in this case, samples of melted subcutaneous fat were analysed in an FOSS XDS NIR rapid content analyser (400 to 2500 nm). Equations based on modified PLS regression methods showed that NIRS technology could predict the fatty acid groups, the main fatty acids and the iodine value accurately with R(2)(cv), RMSECV, R(2)(val) and RMSEP of 0.98, 0.38%, 0.95 and 0.49%, respectively (saturated fatty acids), 0.94, 0.45%, 0.97 and 0.65%, respectively (monounsaturated fatty acids), 0.97, 0.28%, 0.99 and 0.34%, respectively (polyunsaturated fatty acids), 0.76, 0.61%, 0.84 and 0.87%, respectively (palmitic acid, C16:0), 0.75, 0.16%, 0.89 and 0.10%, respectively (palmitoleic acid, C16:1n-7), 0.93, 0.41%, 0.96 and 0.64%, respectively (steric acid, C18:0), 0.90, 0.51%, 0.94 and 0.44%, respectively (oleic acid, C18:1n-9), 0.97, 0.25%, 0.98 and 0.29% (linoleic acid, C18:2n-6), 0.68, 0.09%, 0.57 and 0.16% (α-linolenic acid, C18:3n-3) and 0.97, 0.57, 0.97 and 1.22, respectively (iodine value, calculated). The magnitude of this error showed quite good accuracy using these rapid methods in prediction of the moisture and fatty acid composition of fat from pigs involved in breeding schemes.

[1]  J. García-Olmo,et al.  Determination of the precision of the fatty acid analysis of Iberian pig fat by gas chromatography. Results of a mini collaborative study. , 2002, Meat science.

[2]  J E Guerrero-Ginel,et al.  A feasibility study on the use of near-infrared spectroscopy for prediction of the fatty acid profile in live Iberian pigs and carcasses. , 2009, Meat science.

[3]  H. Büning-Pfaue Analysis of water in food by near infrared spectroscopy , 2003 .

[4]  Shirley Anderson,et al.  Determination of fat, moisture, and protein in meat and meat products by using the FOSS FoodScan Near-Infrared Spectrophotometer with FOSS Artificial Neural Network Calibration Model and Associated Database: collaborative study. , 2007, Journal of AOAC International.

[5]  A. Garrido-Varo,et al.  The Transfer of Fatty Acid Calibration Equations Using Four Sets of Unsealed Liquid Standardisation Samples , 2001 .

[6]  J. Kongsro,et al.  Genetic parameters of fat quality in pigs measured by near-infrared spectroscopy. , 2011, Animal : an international journal of animal bioscience.

[7]  E. De Pedro,et al.  Microwave oven application in the extraction of fat from the subcutaneous tissue of Iberian pig ham. , 1997, Meat science.

[8]  Alex B. McBratney,et al.  Laboratory evaluation of a proximal sensing technique for simultaneous measurement of soil clay and water content , 1998 .

[9]  Emil W. Ciurczak,et al.  Handbook of Near-Infrared Analysis , 1992 .

[10]  Jerome J. Workman,et al.  Application of NIR spectroscopy to agricultural products. In 'Handbook of Near-infrared Analysis'.(E , 1992 .

[11]  T. Næs,et al.  Nondestructive NIR and NIT Determination of Protein, Fat, and Water in Plastic-Wrapped, Homogenized Meat , 1992 .

[12]  J. Shenk,et al.  Application of NIR Spectroscopy to Agricultural Products , 1992 .

[13]  Near Infrared Spectroscopy for Quantification of Animal-Origin Fats in Fat Blends , 2008 .

[14]  C. Rodríguez,et al.  Genetic parameters for meat and fat quality and carcass composition traits in Iberian pigs. , 2003, Meat science.

[15]  I. González-Martín,et al.  Determination of fatty acids in the subcutaneous fat of Iberian breed swine by near infrared spectroscopy (NIRS) with a fibre-optic probe. , 2003, Meat science.

[16]  Christian Paul,et al.  The Repeatability File—A Tool for Reducing the Sensitivity of near Infrared Spectroscopy Calibrations to Moisture Variation , 1998 .

[17]  Sumio Kawano,et al.  Near infrared spectral patterns of fatty acid analysis from fats and oils , 1991 .

[18]  Tormod Næs,et al.  Near Infra-Red Spectroscopy: Bridging the Gap between Data Analysis and NIR Applications , 1995 .

[19]  M. Forina,et al.  Multivariate calibration. , 2007, Journal of chromatography. A.

[20]  O. Vangen,et al.  Genetic parameters of meat quality traits in two pig breeds measured by rapid methods. , 2010, Animal : an international journal of animal bioscience.

[21]  T. Næs,et al.  The Effect of Multiplicative Scatter Correction (MSC) and Linearity Improvement in NIR Spectroscopy , 1988 .

[22]  Jerome J. Workman,et al.  Near-infrared spectroscopy in agriculture , 2004 .

[23]  Inmaculada González-Martín,et al.  Determination of Fatty Acids in the Subcutaneous Fat of Iberian Breed Swine by near Infrared Spectroscopy. A Comparative Study of the Methods for Obtaining Total Lipids: Solvents and Melting with Microwaves , 2002 .

[24]  Dolores Pérez-Marín,et al.  Chemometric utilities to achieve robustness in liquid NIRS calibrations: Application to pig fat analysis , 2007 .