Prevalence of Different Salmonella Serovars in Broiler Farms in Sharkia Governorate

Bacteriological examination of 300samples from broiler internal organs(liver,2ceci,un absorbed yolk sac) from1 day old chicks suffering from omphalitis and respiratory disorders after hatching ,revealed 37 Salmonella isolates 12.3%.Conventional methods for isolation and identification of Salmonella isolates from chicks showed that Salmonella apper as a colorless colonies on Macconkeys agar medium . It gave the characteristics slightly transparent zone of reddish color with or without black center on XLD and colorless colonies with black centre onSS agar medium.It appeared as Gram –negative straight rods , non-spore forming and arranged single in pairs and in groups .Moreover , Salmonella isolates were citrate test positive (blue color),urease test negative (yellow color)and they also gave acid but(yellow) and alkaline slant (red) with H25 production (black correlation on TSI agar medium.Serotyping of 37 Salmonella isolates by slide agglutination test using using specific monovalent and polyvalent O and H Salmonella sera revealed eleven different Salmonella serotypes ,with S. Enteritidis as themost prevalent serotype (27.1%) followed by S.Tamale (16.2%). ,S. Typhymurium and S. Kentucky (13.5%) for each one ,S.Anatum (10.8%),S.Gallinarum 5.4%,while S.Infntis ,S.Bargny ,S.Takorade ,S.Heldberg and S.Larochelle were the last detected serovars (2.7%) for each one. Anti-microbial sensitivity test revealed that most Salmonella isolates were sensitive to Ciprofloxacin ,sulfa methoxasole – trimethoprime , Chlorumphenicol and gentamycin .while they were resistant to Erythromycin,Rifamycin ,Amoxycillin /clavulinic acid ,colistin sulfate and cefotaxime .