Two-photon optical properties of near-infrared dyes at 1.55 μm excitation.

Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.

[1]  P. Wentzell,et al.  Modeling the Response of a Long-Period Fiber Grating to Ambient Refractive Index Change in Chemical Sensing Applications , 2008, Journal of Lightwave Technology.

[2]  S. Achilefu,et al.  Novel receptor-targeted fluorescent contrast agents for in vivo tumor imaging. , 2000, Investigative radiology.

[3]  S. Achilefu,et al.  Dynamic noninvasive monitoring of renal function in vivo by fluorescence lifetime imaging. , 2009, Journal of biomedical optics.

[4]  Jong Seung Kim,et al.  Characterization of Ultrafast Intramolecular Charge Transfer Dynamics in Pyrenyl Derivatives: Systematic Change of the Number of Peripheral N,N-Dimethyaniline Substituents , 2011 .

[5]  B. Strehmel,et al.  The Influence of σ and π Acceptors on Two‐Photon Absorption and Solvatochromism of Dipolar and Quadrupolar Unsaturated Organic Compounds , 2003 .

[6]  J. Perry,et al.  Rapid, broadband two-photon-excited fluorescence spectroscopy and its application to red-emitting secondary reference compounds , 2011 .

[7]  S. Achilefu,et al.  Fluorescence lifetime measurements and biological imaging. , 2010, Chemical reviews.

[8]  W. Denk,et al.  Deep tissue two-photon microscopy , 2005, Nature Methods.

[9]  Samuel Achilefu,et al.  Ratiometric Analysis of Fluorescence Lifetime for Probing Binding Sites in Albumin with Near‐Infrared Fluorescent Molecular Probes , 2007, Photochemistry and photobiology.

[10]  W. Webb,et al.  Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm , 1996 .

[11]  Jie Zheng,et al.  Near infrared-fluorescent and magnetic resonance imaging molecular probe with high T1 relaxivity for in vivo multimodal imaging. , 2010, Chemical communications.

[12]  Samuel Achilefu,et al.  Predicting in vivo fluorescence lifetime behavior of near-infrared fluorescent contrast agents using in vitro measurements. , 2008, Journal of biomedical optics.

[13]  S. Achilefu,et al.  Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems. , 2007, Biophysical journal.

[14]  E Gratton,et al.  Experimental verification of a theory for the time-resolved fluorescence spectroscopy of thick tissues. , 1997, Applied optics.

[15]  A. Ishchenko,et al.  Nonlinear optical characteristics and lasing ability of merocyanine dyes having different solvatochromic behaviour , 2008 .

[16]  M. Drobizhev,et al.  Two-photon absorption standards in the 550-1600 nm excitation wavelength range. , 2008, Optics express.

[17]  Xingde Li,et al.  Compensation-free, all-fiber-optic, two-photon endomicroscopy at 1.55 μm. , 2011, Optics letters.

[18]  Chang-Bong Kim,et al.  Measurement of the refractive index of liquids at 1.3 and 1.5 micron using a fibre optic Fresnel ratio meter , 2004 .

[19]  F. van Mourik,et al.  Multiphoton-excited luminescent lanthanide bioprobes: two- and three-photon cross sections of dipicolinate derivatives and binuclear helicates. , 2010, The journal of physical chemistry. B.

[20]  Min Gu,et al.  Fast handheld two-photon fluorescence microendoscope with a 475 μm × 475 μm field of view for in vivo imaging , 2008 .

[21]  Sylvain Gioux,et al.  Low-frequency wide-field fluorescence lifetime imaging using a high-power near-infrared light-emitting diode light source. , 2010, Journal of biomedical optics.

[22]  R. C. Benson,et al.  Fluorescence properties of indocyanine green as related to angiography. , 1978, Physics in medicine and biology.

[23]  E. Sevick-Muraca,et al.  Fluorescence lifetime spectroscopy in multiply scattering media with dyes exhibiting multiexponential decay kinetics. , 2002, Biophysical journal.

[24]  R. Weissleder,et al.  Charge-coupled-device based scanner for tomography of fluorescent near-infrared probes in turbid media. , 2002, Medical physics.

[25]  Samuel Achilefu,et al.  Fluorescence lifetime properties of near-infrared cyanine dyes in relation to their structures. , 2008, Journal of photochemistry and photobiology. A, Chemistry.

[26]  Samuel Achilefu,et al.  Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging. , 2011, Biochemistry.

[27]  M. Blanchard‐Desce,et al.  Towards "smart" multiphoton fluorophores: strongly solvatochromic probes for two-photon sensing of micropolarity. , 2005, Chemical communications.

[28]  Siavash Yazdanfar,et al.  Multiphoton microscopy with near infrared contrast agents. , 2010, Journal of biomedical optics.