19-nor-deoxycorticosterone: a potent mineralcocorticoid isolated from the urine of rats with regenerating adrenals.

Adrenal regeneration hypertension (ARH) is an experimental form of low renin hypertension in the rat in which an unknown mineralocorticoid (s) appears to contribute to the pathophysiology of the hypertension. To isolate unknown mineralocorticoids, urine of castrated rats with regenerating adrenals was lowered to pH 1.0 with sulfuric acid, incubated for 24-h at room temperature, extracted with ethyl acetate, and subjected to several successive chromatographic purifications. Unknown urinary mineralocorticoid activity was identified and monitored with an aldosterone radioreceptor assay to enable isolation of approximately 1 mg of a pure unknown steroid. Direct probe mass spectrometry, gas chromatography-mass spectrometry, and nuclear magnetic resonance revealed the following characteristics of the isolated steroid: 1) a molecular weight of 316; 2) a deficit in 14 atomic mass units in comparison to deoxycorticosterone (DOC); 21-hydroxy-4-pregnene-3,20-dione) and to the corresponding dimethoxime and dimethoxim...

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