Biocontrol of Meloidogyne sp. on Tomato Plants by Selected Bacillus spp

Meloidogyne spp., are recognize as the most economically important worldwide. It is difficult to control Meloidogyne spp., because they have wide range of hosts. Due to the high toxicity of chemical nematicides, it is necessary to develop new control strategies. Our previous research showed that some Bacillus spp. effective as biocontrol agents of plant pests and diseases. They have multifunction, such as plant growth promoter, phosphate dissolving bacteria, etc. The aim of this research was to obtain the selected Bacillus spp. to control Meloidogyne spp., to enhance growth and yield of tomato. The treatments were 8 strains of Bacillus spp., Carbofuran, Meloidogyne spp. inoculated tomato and control. The Bacillus spp. have inoculated as seed treatment and seedling treatment before transplanting. The Meloidogyne spp. have inoculated on 4 weeks tomato plants. The parameters were disease development, multiplication of Meloidogyne spp., growth and yield of tomato. The results showed that Bacillus spp. reduced the number of galls, egg masses, eggs per egg mass and nematode per 300 g soil compared to the inoculated control, but the nematicide treatments was more effective to control Meloidogyne spp. Conversely, three strains of Bacillus spp. showed to enhance the growth and yield of tomato than Carbofuran.

[1]  N. Wulansari,et al.  MEKANISME ANTAGONIS LIMA ISOLAT Bacillus subtilis TERHADAP Colletotrichum capsici DAN C. gloeospoiroides IN VITRO , 2018 .

[2]  Y. Yanti,et al.  Indigenous Bacillus spp. ability to growth promoting activities and control bacterial wilt disease (Ralstonia solanacearum) , 2017 .

[3]  Zurai Resti,et al.  FORMULASI PADAT RHIZOBAKTERIA INDIGENUS BACILLUS THURINGIENSIS TS2 DAN WAKTU PENYIMPANAN UNTUK MENGENDALIKAN PENYAKIT PUSTUL BAKTERI XANTHOMONAS AXONOPODIS PV. GLYCINES , 2017 .

[4]  W. Warnita,et al.  Identification and Characterizations of Potential Indigenous Endophytic Bacteria which had Ability to Promote Growth Rate of Tomato and Biocontrol Agents of Ralstonia solanacearum and Fusarium oxysporum fsp. solani , 2017 .

[5]  Hannatu K. Ali,et al.  Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum). , 2016, Microbiological research.

[6]  Yunpeng Liu,et al.  Induced maize salt tolerance by rhizosphere inoculation of Bacillus amyloliquefaciens SQR9. , 2016, Physiologia plantarum.

[7]  J. Hao,et al.  Effects of Bacillus velezensis strain BAC03 in promoting plant growth , 2016 .

[8]  M. Abdel-Wahab,et al.  Biocontrol of Meloidogyne javanica on Tomato Plants by Hypocrea lixii (the Teleomorph of Trichoderma harzianum) , 2014 .

[9]  C. K. Shirkot,et al.  Effect of Bacillus subtilis Strain CKT1 as Inoculum on Growth of Tomato Seedlings Under Net House Conditions , 2013, Proceedings of the National Academy of Sciences, India Section B: Biological Sciences.

[10]  S. Nakkeeran,et al.  Effects of crude antibiotic of Bacillus subtilis on hatching of eggs and mortality of juveniles of Meloidogyne incognita , 2012 .

[11]  Abbasi,et al.  Plant growth promoting Rhizobacteria: An overview , 2012 .

[12]  E. Sikora,et al.  Plant root-bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases , 1999, Australasian Plant Pathology.

[13]  Ashoub.  Biocontrol Activity of Some Bacterial Genera Against Root-Knot nematode, Meloidogyne incognita , 2010 .

[14]  P. Oger,et al.  Quorum Sensing and Quorum Quenching: The Yin and Yang of Bacterial Communication , 2009, Chembiochem : a European journal of chemical biology.

[15]  R. Sikora,et al.  Endophytic bacteria from Ethiopian coffee plants and their potential to antagonise Meloidogyne incognita , 2009 .

[16]  N. Sahebani,et al.  Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum , 2008 .

[17]  L. C. Loon.  Plant responses to plant growth-promoting rhizobacteria , 2007, European Journal of Plant Pathology.

[18]  U. Chakraborty,et al.  Plant growth promotion and induction of resistance in Camellia sinensis by Bacillus megaterium , 2006, Journal of basic microbiology.

[19]  J. Kloepper,et al.  Analysis of populations and physiological characterization of microorganisms in rhizospheres of plants with antagonistic properties to phytopathogenic nematodes , 1991, Plant and Soil.

[20]  Z. Siddiqui.  PGPR: Prospective Biocontrol Agents of Plant Pathogens , 2005 .

[21]  Michel Luc,et al.  Plant parasitic nematodes in subtropical and tropical agriculture , 2005 .

[22]  Z. Siddiqui.  Effects of plant growth promoting bacteria and composed organic fertilizers on the reproduction of Meloidogyne incognita and tomato growth. , 2004, Bioresource technology.

[23]  S. Shaukat,et al.  Systemic Resistance in Tomato Induced by Biocontrol Bacteria Against the Root‐Knot Nematode, Meloidogyne javanica is Independent of Salicylic Acid Production , 2004 .

[24]  I. Chet,et al.  Biological Control of the Root-Knot Nematode Meloidogyne javanica by Trichoderma harzianum. , 2001, Phytopathology.

[25]  John F. Murphy,et al.  Microbe-Induced Resistance Against Pathogens and Herbivores : Evidence of Effectiveness in Agriculture , 2001 .

[26]  D. Trudgill,et al.  Plant Parasitic Nematodes in Temperate Agriculture , 1993 .

[27]  B. Brodie,et al.  Nematode parasites of potatoes , 1993 .

[28]  R. Sikora.  Management of the Antagonistic Potential in Agricultural Ecosystems for the Biological Control of Plant Parasitic Nematodes , 1992 .

[29]  D. Sands,et al.  Methods in Phytobacteriology , 1990 .

[30]  R. Sikora,et al.  Nematode parasites of vegetables. , 1990 .

[31]  David M. Weller,et al.  Biological control of soilborne plant pathogens in the rhizosphere with bacteria , 1988 .

[32]  G. Stirling.  Biological control of Meloidogyne javanica with Bacillus penetrans , 1984 .

[33]  J. G. Hancock,et al.  Disease-Suppressive Soil and Root-Colonizing Bacteria , 1982, Science.