국소 자기장의 순/역 배열을 이용한 미세유체 채널 내에서의 자기입자 패턴 형성

An approach is described for a direct patterning of magnetically active microbeads in microfluidic devices to enhance particle and cell capture efficiency. The accurate patterning of diverse microbeads in fluidic channels has a potential impact on the development of new medical devices because of their versatile research applications. The PDMS microchannel without inner structure was put on the arranged magnet zig. Various local magnet arrangements were utilized for generating trapping magnetic fields: flat arrangement of vertically forward magnet arrays; crisscross stack of magnet pads which have horizontally repeatable forward magnet arrangement; flat arrangement of vertically reverse magnet arrays; and X-shaped flat arrangement of vertically reverse magnet. The crisscross stack of magnet pads profiled egg tray-formed magnetic fields. The flat arrangement of vertically forward magnet arrays generated isolated patterns by attracting magnetic field line around an each magnet. The flat arrangement of vertically reverse magnet arrays shaped trapping patterns repelling magnetic field line between same poles. The magnetic field line of X-shaped flat arrangement showed independent trapped pattern in an each magnet. The attracting and repelling properties by various composition of magnetic poles allow diverse isolated trapped patterns of magnetic particles. Trapped magnetic particles in fluidic channels were stable on the 8 ㎖/hr flow conditions in the all experiments. This versatile and simple method has potential to be applicable in in-situ immunomagnetic sorting and non-invasive patterning of samples.