The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis

Angiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research.  

[1]  E. Giovannetti,et al.  Development of bioluminescent chick chorioallantoic membrane (CAM) models for primary pancreatic cancer cells: a platform for drug testing , 2017, Scientific Reports.

[2]  S. Banskota,et al.  Tryptophan hydroxylase 1 and 5-HT7 receptor preferentially expressed in triple-negative breast cancer promote cancer progression through autocrine serotonin signaling , 2016, Molecular Cancer.

[3]  Bo Zhou,et al.  Identification of resveratrol derivative 3,3',4,4',5,5'-hexamethoxy- trans-stilbene as a novel pro-angiogenic small-molecule compound. , 2016, European journal of pharmacology.

[4]  L. Salford,et al.  ITPP Treatment of RG2 Glioblastoma in a Rat Model. , 2016, Anticancer research.

[5]  Rahul D. Kamble,et al.  Evaluation of Curcumin Capped Copper Nanoparticles as Possible Inhibitors of Human Breast Cancer Cells and Angiogenesis: a Comparative Study with Native Curcumin , 2016, AAPS PharmSciTech.

[6]  Xuesong Yang,et al.  Exposure to Excess Phenobarbital Negatively Influences the Osteogenesis of Chick Embryos , 2016, Front. Pharmacol..

[7]  J. Kanczler,et al.  The chorioallantoic membrane (CAM) assay for the study of human bone regeneration: a refinement animal model for tissue engineering , 2016, Scientific Reports.

[8]  R. Dikshit,et al.  IL17 producing γδT cells induce angiogenesis and are associated with poor survival in gallbladder cancer patients , 2016, International journal of cancer.

[9]  M. H. Fernandes,et al.  In vivo assessment of a new multifunctional coating architecture for improved Mg alloy biocompatibility , 2016, Biomedical materials.

[10]  D. Ribatti.  The chick embryo chorioallantoic membrane (CAM). A multifaceted experimental model , 2016, Mechanisms of Development.

[11]  L. Linsen,et al.  Cardiac atrial appendage stem cells promote angiogenesis in vitro and in vivo. , 2016, Journal of molecular and cellular cardiology.

[12]  Hong Sun,et al.  MicroRNA-33a-3p suppresses cell migration and invasion by directly targeting PBX3 in human hepatocellular carcinoma , 2016, Oncotarget.

[13]  Zhenqing Feng,et al.  Effect of AURKA Gene Expression Knockdown on Angiogenesis and Tumorigenesis of Human Ovarian Cancer Cell Lines , 2016, Targeted Oncology.

[14]  M. Degani,et al.  Evaluation of antitumor and anti-angiogenic activity of bioactive compounds from Cinnamomum tamala: In vitro, in vivo and in silico approach , 2016 .

[15]  T. Mitsui,et al.  Generation of bioengineered feather buds on a reconstructed chick skin from dissociated epithelial and mesenchymal cells , 2016, Development, growth & differentiation.

[16]  F. Abas,et al.  Chitosan Nanoparticles as Carriers for the Delivery of ΦKAZ14 Bacteriophage for Oral Biological Control of Colibacillosis in Chickens , 2016, Molecules.

[17]  M. Raica,et al.  Endostatin Effects on Tumor Cells and Vascular Network of Human Renal Cell Carcinoma Implanted on Chick Embryo Chorioallantoic Membrane. , 2015, Anticancer research.

[18]  Xiaoping Zhou,et al.  Metastasis-Associated in Colon Cancer-1 Associates With Poor Prognosis and Promotes Cell Invasion and Angiogenesis in Human Cervical Cancer , 2015, International Journal of Gynecologic Cancer.

[19]  B. Nitzsche,et al.  Antioxidant, antiangiogenic and antiproliferative activities of root methanol extract of Calliandra portoricensis in human prostate cancer cells. , 2015, Journal of integrative medicine.

[20]  G. Luyten,et al.  Use of the Chick Embryo Model in Uveal Melanoma , 2015, Ocular Oncology and Pathology.

[21]  K. B. Machado,et al.  The State-of-Art in Angiogenic Properties of Latex from Different Plant Species , 2015 .

[22]  Hong-Boon Lee,et al.  Chick embryo chorioallantoic membrane (CAM): an alternative predictive model in acute toxicological studies for anti-cancer drugs , 2015, Experimental animals.

[23]  I. Herr,et al.  Ethical euthanasia and short-term anesthesia of the chick embryo. , 2015, ALTEX.

[24]  D. Ribatti.  The chick embryo chorioallantoic membrane as a model for tumor biology. , 2014, Experimental cell research.

[25]  Wei Wu,et al.  Application of the Chick Embryo Chorioallantoic Membrane in Neurosurgery Disease , 2014, International journal of medical sciences.

[26]  Patrycja Nowak-Sliwinska,et al.  The chicken chorioallantoic membrane model in biology, medicine and bioengineering , 2014, Angiogenesis.

[27]  Carlos Frederico de Oliveira Graeff,et al.  Hancornia speciosa latex for biomedical applications: physical and chemical properties, biocompatibility assessment and angiogenic activity , 2014, Journal of Materials Science: Materials in Medicine.

[28]  C. Nansen,et al.  Journal impact factors and the influence of age and number of citations. , 2014, Molecular plant pathology.

[29]  C. Ricciardelli,et al.  Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified Molecules on Ovarian Cancer Invasion and Metastasis , 2012, International journal of molecular sciences.

[30]  T. S. Sampaio,et al.  Evaluation of the Cytotoxic Activity of Some Brazilian Medicinal Plants , 2012, Planta Medica.

[31]  M. Balke,et al.  Morphologic characterization of osteosarcoma growth on the chick chorioallantoic membrane , 2010, BMC Research Notes.

[32]  D. Ribatti.  The Chick Embryo Chorioallantoic Membrane as an In Vivo Assay to Study Antiangiogenesis , 2010, Pharmaceuticals.

[33]  Erin M. Conn,et al.  Evaluation of metastatic and angiogenic potentials of human colon carcinoma cells in chick embryo model systems , 2009, Clinical & Experimental Metastasis.

[34]  Marius Raica,et al.  The chick embryo chorioallantoic membrane as a model to study tumor metastasis , 2008, Angiogenesis.

[35]  L. Neckers,et al.  Azaspirene, a fungal product, inhibits angiogenesis by blocking Raf‐1 activation , 2008, Cancer science.

[36]  K. Shakesheff,et al.  Supercritical carbon dioxide generated vascular endothelial growth factor encapsulated poly(DL-lactic acid) scaffolds induce angiogenesis in vitro. , 2007, Biochemical and biophysical research communications.

[37]  C. Rhee,et al.  Emodin inhibits vascular endothelial growth factor‐A‐induced angiogenesis by blocking receptor‐2 (KDR/Flk‐1) phosphorylation , 2006, International journal of cancer.

[38]  H. Riechelmann,et al.  An autologous system for culturing head and neck squamous cell carcinomas for the assessment of cellular therapies on the chorioallantois membrane , 2006, European Archives of Oto-Rhino-Laryngology and Head & Neck.

[39]  Barry Bozeman,et al.  The Impact of Research Collaboration on Scientific Productivity , 2005 .

[40]  Y. Gho,et al.  Synthetic curcumin analogs inhibit activator protein-1 transcription and tumor-induced angiogenesis. , 2004, Biochemical and biophysical research communications.

[41]  J. Folkman,et al.  Fundamental concepts of the angiogenic process. , 2003, Current molecular medicine.

[42]  Benito Casu,et al.  Short heparin sequences spaced by glycol-split uronate residues are antagonists of fibroblast growth factor 2 and angiogenesis inhibitors. , 2002, Biochemistry.

[43]  C. Yeh,et al.  Agkistin, a Snake Venom-derived Glycoprotein Ib Antagonist, Disrupts von Willebrand Factor-Endothelial Cell Interaction and Inhibits Angiogenesis* , 2000, The Journal of Biological Chemistry.

[44]  D. Cheresh,et al.  Requirement of vascular integrin alpha v beta 3 for angiogenesis. , 1994, Science.

[45]  K. Subramanyam,et al.  Bibliometric studies of research collaboration: A review , 1983 .

[46]  Ying Xia,et al.  Establishment of human hepatocellular carcinoma CAM xenograft model and observation of angiogenesis characteristics , 2016 .

[47]  Fabrício Barreto Teresa,et al.  Publish (in a group) or perish (alone): the trend from single- to multi-authorship in biological papers , 2014, Scientometrics.

[48]  K. B. Machado,et al.  Trends and Biases in Global Climate Change Literature , 2012 .

[49]  P. Carmeliet,et al.  Principles and therapeutic implications of angiogenesis, vasculogenesis and arteriogenesis. , 2006, Handbook of experimental pharmacology.

[50]  Hans-Jürgen Czerwon,et al.  Scientometric indicators for a specialty in theoretical high-energy physics: Monte Carlo methods in lattice field theory , 2005, Scientometrics.

[51]  J. Folkman,et al.  Tumor angiogenesis activity in cells grown in tissue culture. , 1976, Cancer research.