Histochemical Study of Oxidative Enzyme Systems in Teeth and Peridental Tissues

In recent years the occurrence of reduced triphosphopyridine nucleotide diaphorase,' succinic,2-4 malic,3 and lactic5 dehydrogenase in fetal and neonatal tooth buds of various species has been observed histochemically, but there have been no publications on the distribution of these enzymes in more differentiated dental tissues. The present study on odontogenesis in older animals has been undertaken to obtain information about the pattern of diaphorases and several dehydrogenases in erupted teeth and their peridental structures, in the hope that it will contribute to a better understanding of their intermediary metabolism. Mice were selected for this purpose because their lower incisors grow continuously and rapidly throughout the life of the animal, permitting the convenient study of development to complete maturity in the same tooth. Like other rodents, mice are monophyodonts (having one set of teeth), and their dentition consists of one incisor and three molars in each quadrant of the jaws., In the adult mouse, amelogenesis occurs only on the labial surface of the root of the incisor. Correspondingly, only the labial surface is covered by enamel. For details on the development and microscopic anatomy of rodent (rat) teeth, the description by Schour and Massler7 should be consulted.

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