Adhesion of high and low virulence Flavobacterium psychrophilum strains to isolated gill arches of rainbow trout Oncorhynchus mykiss.

The ability of Flavobacterium psychrophilum to adhere to the gill tissue of rainbow trout Oncorhynchus mykiss was evaluated. A gill perfusion model was adopted, offering a number of advantages compared to other in vitro as well as in vivo models. A comparison between the adhesion capacity of a high and low virulence F. psychrophilum strain was made. Experiments were additionally carried out to assess the influence of water quality (organic material, nitrite) and temperature on the adhesion process of the bacterial cells. The high virulence strain attached more readily to the gill tissue than did the low virulence strain. Moreover, the adherence of the high virulence strain of F. psychrophilum was influenced by a number of factors. These were immersion of the gill arches in water to which organic material or nitrite were added, and elevated temperature. The former 2 increased the adhesion ability, while the latter had a negative influence on the adherence process.

[1]  F. Haesebrouck,et al.  An alternative model to study the association of rainbow trout (Oncorhynchus mykiss L.) pathogens with the gill tissue , 2002, Laboratory animals.

[2]  M. Kulomaa,et al.  Diagnosis of flavobacteriosis by direct amplification of rRNA genes. , 2002, Diseases of aquatic organisms.

[3]  H. Nelis,et al.  In vivo study of phagocytosis, intracellular survival and multiplication of Flavobacterium psychrophilum in rainbow trout, Oncorhynchus mykiss (Walbaum), spleen phagocytes , 2001 .

[4]  H. Wakabayashi,et al.  An Investigation on the Distribution of Flavobacterium psychrophilum in the Umikawa River , 2000 .

[5]  F. Haesebrouck,et al.  Difficulties in experimental infection studies with Flavobacterium psychrophilum in rainbow trout (Oncorhynchus mykiss) using immersion, oral and anal challenges. , 2000, Research in veterinary science.

[6]  F. Haesebrouck,et al.  Effect of Flavobacterium psychrophilum strains and their metabolites on the oxidative activity of rainbow trout oncorhynchus mykiss phagocytes. , 2000, Diseases of aquatic organisms.

[7]  I. Dalsgaard,et al.  Bacterial pathogens in rainbow trout, Oncorhynchus mykiss (Walbaum), reared at Danish freshwater farms. , 2000 .

[8]  I. Dalsgaard,et al.  Comparative studies of Danish Flavobacterium psychrophilum isolates: ribotypes, plasmid profiles, serotypes and virulence , 2000 .

[9]  F. Haesebrouck,et al.  Development of a Gill Perfusion Apparatus for Studying the Interaction of Fish Pathogens with Gill Tissue , 2000, Alternatives to laboratory animals : ATLA.

[10]  G. Knudsen,et al.  In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. , 1999 .

[11]  B. Austin,et al.  Bacterial fish pathogens: disease in farmed and wild fish. , 1999 .

[12]  G. Heo,et al.  First Isolation and Identification of Cytophaga psychrophila from Cultured Ayu in Korea , 1998 .

[13]  H. Ferguson,et al.  Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus , 1997 .

[14]  A. Haaparanta,et al.  Gill anomalies of perch and roach from four lakes differing in water quality , 1997 .

[15]  S. Swift,et al.  Continuous ambulatory peritoneal dialysis-associated peritonitis as a model device-related infection: phenotypic adaptation, the staphylococcal cell envelope and infection. , 1995, The Journal of hospital infection.

[16]  L. Schmidtke,et al.  Characteristics of Flexibacter psychrophilus isolated from Atlantic salmon in Australia , 1995 .

[17]  C. Burke,et al.  Responses of fish-pathogenic Cytophaga/Flexibacter-like bacteria (CFLB) to environmental conditions , 1994 .

[18]  D. Bruno.  Cytophaga psychrophila (='Flexibacter psychrophilus')(Borg), histopathology associated with mortalities among farmed rainbow trout, Oncorhynchus mykiss (Walbaum) in the UK , 1992 .

[19]  T. S. Hastings,et al.  Isolation of Cytophaga psychrophila (Flexibacter psychrophilus) in association with rainbow trout mortality in the United Kingdom , 1992 .

[20]  H. Wakabayashi,et al.  Outbreaks of cold-water disease in coho salmon in Japan , 1991 .

[21]  R. J. González,et al.  Interspecific Differences in Gill Morphology of Freshwater Fish in Relation to Tolerance of Low-pH Environments , 1991, Physiological Zoology.

[22]  S. Perry,et al.  Environmental Effects on Fish Gill Morphology , 1991, Physiological Zoology.

[23]  J. Rohovec,et al.  Relation of Water Temperature to Bacterial Cold-Water Disease in Coho Salmon, Chinook Salmon, and Rainbow Trout , 1989 .

[24]  R. Holt.  Cytophaga psychrophila, the causative agent of bacterial cold-water disease in salmonid fish , 1987 .

[25]  A. Decostere.  Flavobacterium columnare infections in fish: the agent and its adhesion to the gill tissue. , 2002, Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie.

[26]  J. Hacker.  Microbial pathogenicity factors as parts of global regulatory networks. (A short review). , 1996, Acta microbiologica et immunologica Hungarica.

[27]  P. Vandamme,et al.  Cutting a gordian knot: Emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom nov (basonym, Cytophaga aquatilis Strohl and Tait 1978). , 1996 .

[28]  R. Rangdale.  Studies on rainbow trout fry syndrome (RTFS) , 1995 .

[29]  J. Montana,et al.  First isolation of Flexibacter psychrophilus, as causative agent of rainbow trout fry syndrome (RTFS), producing rainbow trout mortality in Chile , 1995 .

[30]  P. Párt,et al.  8 Primary Cultures of Teleost Branchial Epithelial Cells , 1995 .

[31]  I. Dalsgaard.  Virulence mechanisms in Cytophaga psychrophila and other Cytophaga-like bacteria pathogenic for fish , 1993 .

[32]  B. Austin,et al.  Systemic disease in turbot Scophthalmus maximus caused by a previously unrecognised Cytophaga like bacterium , 1989 .

[33]  H. Shieh.  Studies on the nutrition of a fish pathogen, Flexibacter columnaris. , 1980 .

[34]  Roberts Rj.  Bacterial diseases of farmed fishes. , 1976 .

[35]  A. F. Borg.  Studies on myxobacteria associated with diseases in salmonid fishes , 1948 .