Guide to Data Acquisition and Analysis

This chapter should provide a first guide to the acquisition and analysis of patch-clamp data Except for the sections on single-channel analysis, the procedures and considerations also hold for data obtained using other voltage-clamp methods. It is assumed that the reader is familiar with the standard methods and terminology of patch-clamp electrophysiology. Because many of the problems that arise during data analysis can be avoided by a proper design of the experiment, including data acquisition, we start by deriving some criteria that should be considered before actually starting to record data.

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