Two Proteins Modulating Transendothelial Migration of Leukocytes Recognize Novel Carboxylated Glycans on Endothelial Cells1

We recently showed that a class of novel carboxylated N-glycans was constitutively expressed on endothelial cells. Activated, but not resting, neutrophils expressed binding sites for the novel glycans. We also showed that a mAb against these novel glycans (mAbGB3.1) inhibited leukocyte extravasation in a murine model of peritoneal inflammation. To identify molecules that mediated these interactions, we isolated binding proteins from bovine lung by their differential affinity for carboxylated or neutralized glycans. Two leukocyte calcium-binding proteins that bound in a carboxylate-dependent manner were identified as S100A8 and annexin I. An intact N terminus of annexin I and heteromeric assembly of S100A8 with S100A9 (another member of the S100 family) appeared necessary for this interaction. A mAb to S100A9 blocked neutrophil binding to immobilized carboxylated glycans. Purified human S100A8/A9 complex and recombinant human annexin I showed carboxylate-dependent binding to immobilized bovine lung carboxylated glycans and recognized a subset of mannose-labeled endothelial glycoproteins immunoprecipitated by mAbGB3.1. Saturable binding of S100A8/A9 complex to endothelial cells was also blocked by mAbGB3.1. These results suggest that the carboxylated glycans play important roles in leukocyte trafficking by interacting with proteins known to modulate extravasation.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  D. Toomre,et al.  A Novel Anionic Modification of N-Glycans on Mammalian Endothelial Cells Is Recognized by Activated Neutrophils and Modulates Acute Inflammatory Responses1 , 2001, The Journal of Immunology.

[3]  H. Rogniaux,et al.  Calcium-induced noncovalently linked tetramers of MRP8 and MRP14 are confirmed by electrospray ionization-mass analysis , 2000, Journal of the American Society for Mass Spectrometry.

[4]  V. Gerke,et al.  A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR. , 2000, Molecular cell.

[5]  C. Kerkhoff,et al.  The Regulatory Role of MRP8 (S100A8) and MRP14 (S100A9) in the Transendothelial Migration of Human Leukocytes , 2000, Pathobiology.

[6]  V. Gerke,et al.  Structural basis of the Ca(2+)-dependent association between S100C (S100A11) and its target, the N-terminal part of annexin I. , 2000, Structure.

[7]  H. J. Harris,et al.  The Annexin Protein Lipocortin 1 Regulates the MAPK/ERK Pathway* , 1999, The Journal of Biological Chemistry.

[8]  R. Hughes Secretion of the galectin family of mammalian carbohydrate-binding proteins. , 1999, Biochimica et biophysica acta.

[9]  C. Kerkhoff,et al.  The Two Calcium-binding Proteins, S100A8 and S100A9, Are Involved in the Metabolism of Arachidonic acid in Human Neutrophils* , 1999, The Journal of Biological Chemistry.

[10]  W. Nacken,et al.  Analysis of the MRP8-MRP14 Protein-Protein Interaction by the Two-hybrid System Suggests a Prominent Role of the C-terminal Domain of S100 Proteins in Dimer Formation* , 1999, The Journal of Biological Chemistry.

[11]  C. Kerkhoff,et al.  Novel insights into structure and function of MRP8 (S100A8) and MRP14 (S100A9). , 1998, Biochimica et biophysica acta.

[12]  M. Perretti,et al.  Promoting detachment of neutrophils adherent to murine postcapillary venules to control inflammation: effect of lipocortin 1. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[13]  M. Perretti,et al.  Lipocortin 1 and chemokine modulation of granulocyte and monocyte accumulation in experimental inflammation. , 1998, General pharmacology.

[14]  M. Perretti,et al.  Novel pathways for glucocorticoid effects on neutrophils in chronic inflammation , 1998, Inflammation Research.

[15]  J. Zaia,et al.  Copurification of P6, MRP8, and MRP14 from human granulocytes and separation of individual proteins. , 1998, Protein expression and purification.

[16]  Takeshi Yoshida,et al.  Intracellular localization of migration inhibitory factor-related protein (MRP) and detection of cell surface MRP binding sites on human leukemia cell lines. , 1998, Journal of biochemistry.

[17]  N. Hogg,et al.  The human S100 protein MRP-14 is a novel activator of the beta 2 integrin Mac-1 on neutrophils. , 1998, Journal of immunology.

[18]  M. Perretti Endogenous mediators that inhibit the leukocyte-endothelium interaction. , 1997, Trends in pharmacological sciences.

[19]  W. Nacken,et al.  The heterodimer of the Ca2+‐binding proteins MRP8 and MRP14 binds arachidonic acid , 1997, FEBS letters.

[20]  M. Hartmann,et al.  Myeloid-related Protein (MRP) 8 and MRP14, Calcium-binding Proteins of the S100 Family, Are Secreted by Activated Monocytes via a Novel, Tubulin-dependent Pathway* , 1997, The Journal of Biological Chemistry.

[21]  J. Saurat,et al.  A Heterocomplex Formed by the Calcium-binding Proteins MRP8 (S100A8) and MRP14 (S100A9) Binds Unsaturated Fatty Acids with High Affinity* , 1997, The Journal of Biological Chemistry.

[22]  R. Hannon,et al.  Mobilizing lipocortin 1 in adherent human leukocytes downregulates their transmigration , 1996, Nature Medicine.

[23]  P. Guyre,et al.  Evidence for specific annexin I-binding proteins on human monocytes. , 1996, The Biochemical journal.

[24]  C. Heizmann,et al.  The S100 family of EF-hand calcium-binding proteins: functions and pathology. , 1996, Trends in biochemical sciences.

[25]  D. Schlaepfer,et al.  Calcium-dependent Binding of S100C to the N-terminal Domain of Annexin I (*) , 1996, The Journal of Biological Chemistry.

[26]  R. Faull Adhesion molecules in health and disease. , 1995, Australian and New Zealand journal of medicine.

[27]  D. Toomre,et al.  Unusual Anionic N-Linked Oligosaccharides from Bovine Lung (*) , 1995, The Journal of Biological Chemistry.

[28]  K. Mahnke,et al.  Heterodimers of the calcium‐binding proteins MRP8 and MRP14 are expressed on the surface of human monocytes upon adherence to fibronectin and collagen. Relation to TNF‐α, IL‐6, and superoxide production , 1995, Journal of leukocyte biology.

[29]  H. Pollard,et al.  Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteins. , 1994, Biochimica et biophysica acta.

[30]  R. Jonsson,et al.  Measurement of plasma calprotectin as an indicator of arthritis and disease activity in patients with inflammatory rheumatic diseases. , 1994, The Journal of rheumatology.

[31]  R. Flower,et al.  Lipocortin-1: cellular mechanisms and clinical relevance. , 1994, Trends in pharmacological sciences.

[32]  W. Wang,et al.  Role of the amino-terminal domain in regulating interactions of annexin I with membranes: effects of amino-terminal truncation and mutagenesis of the phosphorylation sites. , 1994, Biochemistry.

[33]  A. Burlingame,et al.  Mass spectrometric and Edman sequencing of lipocortin I isolated by two-dimensional SDS/PAGE of human melanoma lysates. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[34]  B. Tümmler,et al.  Complex pattern of the myelo-monocytic differentiation antigens MRP8 and MRP14 during chronic airway inflammation. , 1992, Immunobiology.

[35]  M. Markert,et al.  Translocation of a small cytosolic calcium-binding protein (MRP-8) to plasma membrane correlates with human neutrophil activation. , 1992, The Journal of biological chemistry.

[36]  I. Weissman,et al.  Mouse MRP8 and MRP14, two intracellular calcium-binding proteins associated with the development of the myeloid lineage. , 1992, Blood.

[37]  M. Karas,et al.  Calcium-dependent complex assembly of the myeloic differentiation proteins MRP-8 and MRP-14. , 1991, The Journal of biological chemistry.

[38]  N. Hogg,et al.  Identification of p8,14 as a highly abundant heterodimeric calcium binding protein complex of myeloid cells. , 1991, The Journal of biological chemistry.

[39]  T. Rapoport,et al.  A novel pathway for secretory proteins? , 1990, Trends in biochemical sciences.

[40]  C. Pallen,et al.  Calcium-dependent and phosphorylation-stimulated proteolysis of lipocortin I by an endogenous A431 cell membrane protease. , 1989, The Journal of biological chemistry.

[41]  N. Hogg,et al.  Monoclonal antibody 5.5 reacts with p8,14, a myeloid molecule associated with some vascular endothelium , 1989, European journal of immunology.

[42]  C. Sorg,et al.  Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues. , 1988, Clinical and experimental immunology.

[43]  E. Lagasse,et al.  Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation , 1988, Molecular and cellular biology.

[44]  R. G. Clerc,et al.  Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis , 1987, Nature.

[45]  B. Mroczkowski,et al.  A calcium-dependent 35-kilodalton substrate for epidermal growth factor receptor/kinase isolated from normal tissue. , 1986, The Journal of biological chemistry.

[46]  J. V. Staros,et al.  N-hydroxysulfosuccinimide active esters: bis(N-hydroxysulfosuccinimide) esters of two dicarboxylic acids are hydrophilic, membrane-impermeant, protein cross-linkers. , 1982, Biochemistry.

[47]  T. Springer,et al.  Traffic signals on endothelium for lymphocyte recirculation and leukocyte emigration. , 1995, Annual review of physiology.

[48]  E. Vollmer,et al.  MRP8 and MRP14, S-100-like proteins associated with myeloid differentiation, are translocated to plasma membrane and intermediate filaments in a calcium-dependent manner. , 1993, Blood.

[49]  C. Hayward,et al.  The Genetical Society Abstracts of Papers presented at the Two Hundred and Second Meeting of the Society on the 1st, 2nd and 3rd April 1985 at the University of Edinburgh , 1985, Heredity.

[50]  Research Paper Mediators of Inflammation, 8, 53–62 (1999) , 2022 .

[51]  Endothelium: Involvement of the Inhibits Their Adhesion to Microvascular Annexin 1 Binds to U937 Monocytic Cells and , 2022 .