Selective Phenotyping Traits Related to Multiple Stress and Drought Response in Dry Bean

Abiotic stress tolerance in dry bean (Phaseolus vulgaris L.) is complex. Increased population sizes are contributing to finding QTL condition - ing stress response but phenotyping has not kept pace with high throughput genotyping for such studies. Our objectives were to deter- mine effectiveness of 20 most tolerant and 20 most susceptible lines representing phenotypic extremes from a RIL population ('Buster' ´ 'Roza' (BR)) to facilitate examination of 19 traits for relevance to stress response and to vali- date existing QTL conditioning stress response. Using phenotypic extremes tested across multi- ple trials, eight of the 19 traits were clearly asso- ciated with drought stress. Pod wall ratio (PW), plant biomass by weight or a visual rating, and greenness index (NDVI) were most associated with seed yield (SY) under stress followed by phenology traits. The phenotypic extreme lines were also useful for validating QTL previously identified in the whole RIL population condi - tioning SY, seed weight (SW) and days to flower (DF), harvest maturity (HM), and seed fill (DSF). New QTL were identified for biomass, PW, and NDVI which co-segregated with major QTL for seed yield SY1.1 BR and SY2.1 BR . The preliminary finding of NDVI 1.1 BR supports aerial imaging in larger genetic populations geared toward QTL analysis of stress response. In summary, phe- notypic extremes helped sort through traits rel- evant to stress response in the BusterRoza RIL population and verified the effect of two major QTL in response to terminal drought.

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