Impulsive Brillouin microscopy

Brillouin scattering has been emerging as a viable tool for microscopy. However, most of the work done has been with the use of spontaneous Brillouin scattering, which has several hindrances to its use. In this work, we propose and demonstrate nonlinear Brillouin scattering as a solution to many of these hindrances. Here we demonstrate fast two-dimensional microscopic optical imaging of materials’ mechanical properties for the very first time (to our knowledge) using nonlinear Brillouin scattering. Impulsive stimulated Brillouin scattering (ISBS) was used in an optical configuration that is capable of providing accurate local assessment of viscoelastic properties faster than conventional Brillouin spectroscopy. This proof-of-principle imaging experiment has been demonstrated for materials of known properties and microfluidic devices. Applications to noninvasive biomedical imaging are discussed. The fast acquisition times and strong signal of ISBS coupled with the ability of Brillouin scattering to easily measure materials’ viscoelastic properties make this an attractive technique for biological use.

[1]  Vladislav V. Yakovlev,et al.  Seeing cells in a new light: a renaissance of Brillouin spectroscopy , 2016 .

[2]  Zhaokai Meng,et al.  Precise Determination of Brillouin Scattering Spectrum Using a Virtually Imaged Phase Array (VIPA) Spectrometer and Charge-Coupled Device (CCD) Camera , 2016, Applied spectroscopy.

[3]  Itay Remer,et al.  Background-free Brillouin spectroscopy in scattering media at 780  nm via stimulated Brillouin scattering. , 2016, Optics letters.

[4]  Zhaokai Meng,et al.  Subcellular measurements of mechanical and chemical properties using dual Raman‐Brillouin microspectroscopy , 2016, Journal of biophotonics.

[5]  Vladislav V. Yakovlev,et al.  Stimulated Brillouin Scattering Microscopic Imaging , 2015, Scientific Reports.

[6]  V. Yakovlev,et al.  Flow cytometry using Brillouin imaging and sensing via time-resolved optical (BISTRO) measurements. , 2015, The Analyst.

[7]  Marlan O Scully,et al.  Dual Raman-Brillouin Microscope for Chemical and Mechanical Characterization and Imaging. , 2015, Analytical chemistry.

[8]  Vladislav V. Yakovlev,et al.  Optimizing signal collection efficiency of the VIPA-based Brillouin spectrometer , 2015 .

[9]  Vladislav V. Yakovlev,et al.  High-speed assessment of liquid viscoelasticity in flow cytometry using nonlinear Brillouin spectroscopy , 2015, Photonics West - Biomedical Optics.

[10]  Vladislav V. Yakovlev,et al.  Background clean-up in Brillouin microspectroscopy of scattering medium. , 2014, Optics express.

[11]  Kristie J. Koski,et al.  Non-invasive determination of the complete elastic moduli of spider silks. , 2013, Nature materials.

[12]  K. Nelson,et al.  Phase-controlled, heterodyne laser-induced transient grating measurements of thermal transport properties in opaque material , 2011, 1109.6685.

[13]  S. Yun,et al.  Multistage VIPA etalons for high-extinction parallel Brillouin spectroscopy , 2011, Optics express.

[14]  Conor L Evans,et al.  Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[15]  K. Nelson,et al.  Optical heterodyne detection of laser-induced gratings. , 1998, Optics letters.

[16]  J. Rogers,et al.  Optical system for rapid materials characterization with the transient grating technique: Application to nondestructive evaluation of thin films used in microelectronics , 1997 .

[17]  M. Shirasaki Large angular dispersion by a virtually imaged phased array and its application to a wavelength demultiplexer. , 1996, Optics letters.

[18]  M. Fayer Picosecond holographic grating generation of ultrasonic waves , 1986 .

[19]  S. Lindsay,et al.  The speed of sound in DNA , 1984, Biopolymers.

[20]  Keith A. Nelson,et al.  Optical generation of tunable ultrasonic waves , 1982 .

[21]  K. Nelson,et al.  Laser-induced phonon spectroscopy. Optical generation of ultrasonic waves and investigation of electronic excited-state interactions in solids , 1981 .

[22]  Keith A. Nelson,et al.  Laser induced phonons: A probe of intermolecular interactions in molecular solids , 1980 .

[23]  J. Randall,et al.  Brillouin scattering, density and elastic properties of the lens and cornea of the eye , 1980, Nature.

[24]  J. White,et al.  Phonons and the elastic moduli of collagen and muscle , 1977, Nature.

[25]  H. Hance,et al.  Wide‐Band Modulation of a Laser Beam, Using Bragg‐Angle Diffraction by Amplitude‐Modulated Ultrasonic Waves , 1965 .

[26]  E. Gross Splitting of the Frequency of Light scattered by Liquids and Optical Anisotropy of Molecules. , 1930, Nature.

[27]  E. Gross The Splitting of Spectral Lines at Scattering of Light by Liquids. , 1930, Nature.

[28]  H. L. Brose,et al.  The Atomic Diameters of Hydrogen and the Inert Gases with respect to Electrons of Very Low Velocity. , 1930, Nature.

[29]  E. Gross Change of Wave-length of Light due to Elastic Heat Waves at Scattering in Liquids. , 1930, Nature.

[30]  S. Yun,et al.  Confocal Brillouin microscopy for three-dimensional mechanical imaging. , 2007, Nature photonics.

[31]  L. Brillouin Diffusion de la lumière et des rayons X par un corps transparent homogène - Influence de l'agitation thermique , 1922 .

[32]  Supplementary Note 1: Longitudinal Modulus , 2022 .