Selective protein transport: identity of the solubilized phosvitin receptor from chicken oocytes.

By two independent methods, the solubilized receptor for phosvitin (PV) has a subunit MW of 116K. Affinity chromatography, showed that only 2 of the more than 25 proteins present in the total detergent solubilized oocyte membrane extract were retained on a PV-agarose column. These proteins of MW of 116K and 100K could be eluted from PV-agarose with free PV. By gel exclusion chromatography, the receptor-125I-PV complexes elute in the void volume of a Biogel A-1.5 column. When these void fractions were assayed gy SDS-PAGE only a single protein of MW of 116K was observed in addition to 125I-PV.

[1]  I. Pastan,et al.  alpha 2 Macroglobulin binding to the plasma membrane of cultured fibroblasts. Diffuse binding followed by clustering in coated regions , 1979, The Journal of cell biology.

[2]  L. Orci,et al.  Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[3]  A. Evans,et al.  Electron microscope observations on the ovarian follicle of the domestic fowl during the rapid growth phase. , 1978, Journal of anatomy.

[4]  Richard G. W. Anderson,et al.  Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts , 1977, Cell.

[5]  R. Rodewald.  pH-dependent binding of immunoglobulins to intestinal cells of the neonatal rat , 1976, The Journal of cell biology.

[6]  M. Brown,et al.  Localization of low density lipoprotein receptors on plasma membrane of normal human fibroblasts and their absence in cells from a familial hypercholesterolemia homozygote. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[7]  R. Wallace,et al.  Protein incorporation by isolated amphibian oocytes. V. Specificity for vitellogenin incorporation , 1976, The Journal of cell biology.

[8]  M. Schlamowitz,et al.  Comparison of the binding affinities of rabbit IgG fractions to the rabbit fetal yolk sac membrane: use of 22Na to facilitate quantitation of 125I-IgG binding. , 1975, Journal of immunology.

[9]  R. Rodewald.  INTESTINAL TRANSPORT OF ANTIBODIES IN THE NEWBORN RAT , 1973, The Journal of cell biology.

[10]  M. Schlamowitz,et al.  Specificity of the in vitro binding of IgG and rabbit serum albumin (RSA) to rabbit yolk sac membrane. , 1972, Journal of immunology.

[11]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[12]  W. Telfer,et al.  An autoradiographic analysis of yolk deposition in the cortex of the cecropia moth oocyte , 1969 .

[13]  R. Wallace,et al.  The induced synthesis and transport of yolk proteins and their accumulation by the oocyte in Xenopus laevis , 1968, Journal of cellular physiology.

[14]  K. Porter,et al.  YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L , 1964, The Journal of cell biology.

[15]  R. Munn,et al.  LIVER SYNTHESIS, PLASMA TRANSPORT, AND STRUCTURAL ALTERATIONS ACCOMPANYING PASSAGE OF YOLK PROTEINS , 1963 .

[16]  W. Telfer.  THE ROUTE OF ENTRY AND LOCALIZATION OF BLOOD PROTEINS IN THE OOCYTES OF SATURNIID MOTHS , 1961, The Journal of biophysical and biochemical cytology.

[17]  J. W. Woods,et al.  Selective protein transport: characterization and solubilization of the phosvitin receptor from chicken oocytes. , 1979, Journal of supramolecular structure.

[18]  T. F. Roth,et al.  Binding to specific receptors on oocyte plasma membranes by serum phosvitin-lipovitellin. , 1976, Journal of supramolecular structure.

[19]  T. F. Roth,et al.  Protein transport: a selective membrane mechanism. , 1976, Journal of supramolecular structure.