Acoustofluidic devices controlled by cell phones.

Acoustofluidic devices have continuously demonstrated their potential to impact medical diagnostics and lab-on-a-chip applications. To bring these technologies to real-world applications, they must be made more accessible to end users. Herein, we report on the effort to provide an easy-to-use and portable system for controlling sharp-edge-based acoustofluidic devices. With the use of a cell phone and a modified Bluetooth® speaker, on-demand and hands-free pumping and mixing are achieved. Additionally, a novel design for a sharp-edge-based acoustofluidic device is proposed that combines both pumping and mixing functions into a single device, thus removing the need for external equipment typically needed to accomplish these two tasks. These applications serve to demonstrate the potential function that acoustofluidic devices can provide in point-of-care platforms. To further this point-of-care goal, we also design a portable microscope that combines with the cell phone and Bluetooth® power supply, providing a completely transportable acoustofluidic testing station. This work serves to bolster the promising position that acoustofluidic devices have within the rapidly changing research and diagnostics fields.

[1]  Aydogan Ozcan,et al.  Mobile phones democratize and cultivate next-generation imaging, diagnostics and measurement tools. , 2014, Lab on a chip.

[2]  Francesco Costanzo,et al.  Investigation of micromixing by acoustically oscillated sharp-edges. , 2016, Biomicrofluidics.

[3]  Leslie Y Yeo,et al.  Microfluidic devices for bioapplications. , 2011, Small.

[4]  G. Whitesides,et al.  Simple telemedicine for developing regions: camera phones and paper-based microfluidic devices for real-time, off-site diagnosis. , 2008, Analytical chemistry.

[5]  Daniel A. Fletcher,et al.  Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope , 2015, Science Translational Medicine.

[6]  Chun Yang,et al.  DC-biased AC-electroosmotic and AC-electrothermal flow mixing in microchannels. , 2009, Lab on a chip.

[7]  T. Huang,et al.  A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures. , 2015, Lab on a chip.

[8]  Harvey Friedman,et al.  Smart Cup: A Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostic Device. , 2016, Sensors and actuators. B, Chemical.

[9]  Y. K. Cheung,et al.  1 Supplementary Information for : Microfluidics-based diagnostics of infectious diseases in the developing world , 2011 .

[10]  Liwei Lin,et al.  Active microfluidic mixer and gas bubble filter driven by thermal bubble micropump , 2002 .

[11]  Hongying Zhu,et al.  Optical imaging techniques for point-of-care diagnostics. , 2013, Lab on a chip.

[12]  Bingcheng Lin,et al.  Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization. , 2010, Lab on a chip.

[13]  Deirdre R Meldrum,et al.  Spontaneous, oscillatory liquid transport in surface tension-confined microfluidics. , 2009, Lab on a chip.

[14]  Po-Hsun Huang,et al.  A single-layer, planar, optofluidic switch powered by acoustically driven, oscillating microbubbles. , 2012, Applied physics letters.

[15]  Luke P. Lee,et al.  Innovations in optical microfluidic technologies for point-of-care diagnostics. , 2008, Lab on a chip.

[16]  Tony Jun Huang,et al.  "Microfluidic drifting"--implementing three-dimensional hydrodynamic focusing with a single-layer planar microfluidic device. , 2007, Lab on a chip.

[17]  Subra Suresh,et al.  Three-dimensional manipulation of single cells using surface acoustic waves , 2016, Proceedings of the National Academy of Sciences.

[18]  Peng Li,et al.  Surface acoustic wave microfluidics. , 2013, Lab on a chip.

[19]  Francesco Costanzo,et al.  Investigation of acoustic streaming patterns around oscillating sharp edges. , 2014, Lab on a chip.

[20]  Chang Liu,et al.  Micro magnetic stir-bar mixer integrated with parylene microfluidic channels. , 2004, Lab on a chip.

[21]  T. Huang,et al.  Cell separation using tilted-angle standing surface acoustic waves , 2014, Proceedings of the National Academy of Sciences.

[22]  I-Kao Chiang,et al.  On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves , 2012, Proceedings of the National Academy of Sciences.

[23]  Pedro Estrela,et al.  Point-of-Care Diagnostics in Low Resource Settings: Present Status and Future Role of Microfluidics , 2015, Biosensors.

[24]  Michael G. Mauk,et al.  Instrument-Free Point-of-Care Molecular Detection of Zika Virus , 2016, Analytical chemistry.

[25]  David J Beebe,et al.  A passive pumping method for microfluidic devices. , 2002, Lab on a chip.

[26]  Gwo-Bin Lee,et al.  Enhancement of Electrokinetically-Driven Flow Mixing in Microchannel with Added Side Channels , 2005 .

[27]  Peng Li,et al.  Controlling cell–cell interactions using surface acoustic waves , 2014, Proceedings of the National Academy of Sciences.

[28]  Po-Hsun Huang,et al.  Acoustofluidic Rotational Manipulation of Cells and Organisms Using Oscillating Solid Structures. , 2016, Small.

[29]  Leslie Y Yeo,et al.  Ultrafast microfluidics using surface acoustic waves. , 2009, Biomicrofluidics.

[30]  Zhenping Wu,et al.  Drastic sensing enhancement using acoustic bubbles for surface-based microfluidic sensors , 2017 .

[31]  Armando R Tovar,et al.  Lateral cavity acoustic transducer. , 2009, Lab on a chip.

[32]  Peng Li,et al.  An acoustofluidic sputum liquefier. , 2015, Lab on a chip.

[33]  Yuliang Xie,et al.  Single-shot characterization of enzymatic reaction constants Km and kcat by an acoustic-driven, bubble-based fast micromixer. , 2012, Analytical chemistry.

[34]  David Sinton,et al.  Hand-powered microfluidics: A membrane pump with a patient-to-chip syringe interface. , 2012, Biomicrofluidics.

[35]  Gwo-Bin Lee,et al.  Electrokinetically driven active micro-mixers utilizing zeta potential variation induced by field effect , 2004 .

[36]  Li Shen,et al.  Point-of-care colorimetric detection with a smartphone. , 2012, Lab on a chip.

[37]  Daniel Ahmed,et al.  Acoustic tweezers: patterning cells and microparticles using standing surface acoustic waves (SSAW). , 2009, Lab on a chip.

[38]  Jeremy Templeton,et al.  Model based design of a microfluidic mixer driven by induced charge electroosmosis. , 2008, Lab on a chip.

[39]  Nam-Trung Nguyen,et al.  Rapid magnetofluidic mixing in a uniform magnetic field. , 2012, Lab on a chip.

[40]  Peter Maunz,et al.  Arrays of individually controlled ions suitable for two-dimensional quantum simulations , 2016, Nature Communications.

[41]  Samuel K Sia,et al.  Commercialization of microfluidic point-of-care diagnostic devices. , 2012, Lab on a chip.

[42]  Tony Jun Huang,et al.  An acoustofluidic micromixer based on oscillating sidewall sharp-edges. , 2013, Lab on a chip.

[43]  Aydogan Ozcan,et al.  Cellphone-based devices for bioanalytical sciences , 2014, Analytical and Bioanalytical Chemistry.

[44]  T. Huang,et al.  Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. , 2009, Lab on a chip.

[45]  Nam-Trung Nguyen,et al.  Micromixers?a review , 2005 .

[46]  Patrick Hunziker,et al.  Valves for autonomous capillary systems , 2008 .

[47]  L. Gervais,et al.  Toward one-step point-of-care immunodiagnostics using capillary-driven microfluidics and PDMS substrates. , 2009, Lab on a chip.

[48]  Daniel Ahmed,et al.  Tunable, pulsatile chemical gradient generation via acoustically driven oscillating bubbles. , 2013, Lab on a chip.

[49]  David Sinton,et al.  High-efficiency electrokinetic micromixing through symmetric sequential injection and expansion. , 2006, Lab on a chip.

[50]  Dazhi Wang,et al.  Electrothermal stirring for heterogeneous immunoassays. , 2005, Lab on a chip.

[51]  Tony Jun Huang,et al.  Microfluidic diagnostics for the developing world. , 2012, Lab on a chip.

[52]  Point-of-Care Technologies for the Advancement of Precision Medicine in Heart, Lung, Blood, and Sleep Disorders , 2016 .

[53]  Daniel Ahmed,et al.  Rotational manipulation of single cells and organisms using acoustic waves , 2016, Nature Communications.

[54]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[55]  Je-Kyun Park,et al.  Pipetting-driven microfluidic immunohistochemistry to facilitate enhanced immunoreaction and effective use of antibodies. , 2017, Lab on a chip.

[56]  George M Whitesides,et al.  Pumping fluids in microfluidic systems using the elastic deformation of poly(dimethylsiloxane). , 2007, Lab on a chip.

[57]  Lin Wang,et al.  A reliable and programmable acoustofluidic pump powered by oscillating sharp-edge structures. , 2014, Lab on a chip.

[58]  Ali Khademhosseini,et al.  Integrating microfluidics and lensless imaging for point-of-care testing , 2009, 2009 IEEE 35th Annual Northeast Bioengineering Conference.

[59]  K. Lei,et al.  Bubble-driven mixer integrated with a microfluidic bead-based ELISA for rapid bladder cancer biomarker detection , 2014, Biomedical microdevices.

[60]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[61]  Hongying Zhu,et al.  Optofluidic fluorescent imaging cytometry on a cell phone. , 2011, Analytical chemistry.

[62]  Jörg P Kutter,et al.  AC electroosmotic pump with bubble-free palladium electrodes and rectifying polymer membrane valves. , 2006, Lab on a chip.

[63]  S M Gruner,et al.  Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[64]  Xiyuan Liu,et al.  Embroidered electrochemical sensors for biomolecular detection. , 2016, Lab on a chip.

[65]  Hyung Jin Sung,et al.  Adjustable, rapidly switching microfluidic gradient generation using focused travelling surface acoustic waves , 2014 .

[66]  Vasan Venugopalan,et al.  Laser-induced mixing in microfluidic channels. , 2007, Analytical chemistry.

[67]  Xingli He,et al.  High sensitivity flexible Lamb-wave humidity sensors with a graphene oxide sensing layer. , 2015, Nanoscale.

[68]  Luke P. Lee,et al.  Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip , 2017, Science Advances.

[69]  Danny van Noort,et al.  Cells in microfluidics. , 2011, Topics in current chemistry.

[70]  Subra Suresh,et al.  Isolation of exosomes from whole blood by integrating acoustics and microfluidics , 2017, Proceedings of the National Academy of Sciences.

[71]  W. Qiu,et al.  Integration of cell phone imaging with microchip ELISA to detect ovarian cancer HE4 biomarker in urine at the point-of-care. , 2011, Lab on a chip.