The effects of cyclophosphamide and irradiation singly and in combination upon SaI growth in A/J mice.

The effects of various doses of cyclophosphamide and low-dose (15 rads) radiation upon the size of tumors caused by 10(4) Sarcoma I (SaI) cells was determined. In intact A/Jax (A/J) recipients, the effect of the two agents singly and in combination was found to be dependent especially upon the dosage of cyclophosphamide and the time of its administration in relation to tumor inoculation. In cell transfer experiments to adult thymectomized, lethally irradiated, bone-marrow-restored (ATxXBM) mice, the effects of cyclophosphamide and irradiation appeared to be either overlapping (low dosages of cyclophosphamide) or additive (dosages of cyclophosphamide greater than or equal to 50 mg/kg), suggesting that the two agents exert their influence in dissimilar fashion, perhaps by injuring different cell types with the same basic function. The most pronounced conjoint effects are seen when low dosages of cyclophosphamide are given 3 days after the adoptive transfer of spleen cells from mice pretreated with low-dose irradiation. The implications of this observation with respect to immunotherapy are discussed.

[1]  R. Anderson,et al.  The structural and functional assessment of cytotoxic injury of the immune system with particular reference to the effects of ionizing radiation and cyclophosphamide. , 1986, The British journal of cancer. Supplement.

[2]  C. Song,et al.  A correlation between conditioning and engraftment in recipients of MHC-mismatched T cell-depleted murine bone marrow transplants. , 1985, Journal of immunology.

[3]  S. Tlouzeau,et al.  Role of T suppressor cells in the cycling of the immune response against a murine fibrosarcoma , 1984, International journal of cancer.

[4]  I. Bursuker,et al.  Generation and decay of the immune response to a progressive fibrosarcoma. I. Ly-1+2- suppressor T cells down-regulate the generation of Ly-1-2+ effector T cells , 1984, The Journal of experimental medicine.

[5]  J. Cowens,et al.  Inhibition of the development of suppressor cells in culture by 4-hydroperoxycyclophosphamide , 1984, The Journal of Immunology.

[6]  M. Greene,et al.  Suppressor T cells and the immune response to tumors. , 1984, Critical reviews in immunology.

[7]  R. Anderson,et al.  Radiation-induced augmentation of the response of A/J mice to SaI tumor cells. , 1982, The American journal of pathology.

[8]  L. Lanier,et al.  Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. , 1981, Journal of immunology.

[9]  M. Boccadoro,et al.  Reduced tumor growth after low-dose irradiation or immunization against blastic suppressor T cells. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[10]  F. Liew,et al.  Differential sensitivity to cyclophosphamide of helper T cells for humoral responses and suppressor T cells for delayed‐type hypersensitivity , 1980 .

[11]  R. Anderson,et al.  Radiation-induced augmentation of the immune response. , 1980, Contemporary topics in immunobiology.

[12]  M. Glaser.  Augmentation of specific immune response against a syngeneic SV40-induced sarcoma in mice by depletion of suppressor T cells with cyclophosphamide. , 1979, Cellular immunology.

[13]  L. Herzenberg,et al.  Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens * , 1979, Immunological reviews.

[14]  K. Hellström,et al.  Antitumor effect of whole-body X-irradiation: possible role of an X-ray-sensitive T suppressor cell population. , 1979, Transplantation proceedings.

[15]  B. Benacerraf,et al.  Regulation of the immune response to tumor antigen. V. Modulation of suppressor t-cell activity in vivo. , 1979 .

[16]  J. Kant,et al.  Regression and inhibition of sarcoma growth by interference with a radiosensitive T-cell population , 1978, The Journal of experimental medicine.

[17]  I. Hellstrom,et al.  Evidence that tumor antigens enhance tumor growth in vivo by interacting with a radiosensitive (suppressor?) cell population. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[18]  D. Katz,et al.  Hapten-specific IgE antibody responses in mice. VI. Selective enhancement of IgE antibody production by low doses of X-irradiation and by cyclophosphamide. , 1976, Journal of immunology.

[19]  J. Bach.  The mode of action of immunosuppressive agents. , 1975, Frontiers of biology.

[20]  F. James Rohlf,et al.  Biometry: The Principles and Practice of Statistics in Biological Research , 1969 .

[21]  M. Lazzarino,et al.  [The mode of action of immunosuppressive agents. 3. Study of the phase of the immunological response sensitive to treatment with corticosteroids]. , 1967, Rivista di emoterapia ed immunoematologia.