Electrochemical sensor for monitoring the photodegradation of catechol based on DNA-modified graphene oxide

AbstractWe have immobilized DNA on a glassy carbon electrode (GCE) modified with graphene oxide (GO) to develop an electrochemical biosensor for catechol. Compared to carbon nanotubes, the use of GO dramatically improved the electrooxidative current of the guanine and adenine moieties in DNA but retained the low background current of unmodified GCEs. Factors such as DNA adsorption time, DNA concentration and pH of solution were investigated to optimize experimental conditions. In the presence of catechol, the voltammetric response to DNA was inhibited due to the interaction between DNA and catechol. The response to adenine is linearly proportional to the concentration of catechol in the range from 1.0 × 10−6 to 1.0 × 10−4 mol·L−1. If catechol is degraded by the combined action of UV light and hydrogen peroxide, the response to DNA is restored. Thus, the modified electrode can act as an efficient biosensor for monitoring the degradation of catechol. FigureGO dramatically improved the electrooxidative current of the guanine and adenine moieties in DNA but retained the low background current of unmodified GCEs. While the DNA/GO-modified electrode was applied to monitor catechol, it showed sensitive response to catechol before and after photodegradation treatment.

[1]  A. Bard,et al.  Electrochemical studies of the interaction of metal chelates with DNA. 4. Voltammetric and electrogenerated chemiluminescent studies of the interaction of tris(2,2'-bipyridine)osmium(II) with DNA. , 1990, Analytical chemistry.

[2]  Changzhu Yang,et al.  Carbon nanotube-based DNA biosensor for monitoring phenolic pollutants , 2009 .

[3]  Gustavo Rivas,et al.  DNA electrochemical biosensors for environmental monitoring. A review , 1997 .

[4]  Y. Wan,et al.  Reduced graphene sheets modified glassy carbon electrode for electrocatalytic oxidation of hydrazine in alkaline media , 2010 .

[5]  P. He,et al.  Electrochemical detection of DNA hybridization based on silver-enhanced gold nanoparticle label , 2002 .

[6]  G Marrazza,et al.  Electrochemical DNA biosensor as a screening tool for the detection of toxicants in water and wastewater samples. , 2002, Talanta.

[7]  G. Rivas,et al.  Carbon nanotubes for electrochemical biosensing. , 2007, Talanta.

[8]  M. Fojta.  Electrochemical Sensors for DNA Interactions and Damage , 2002 .

[9]  D. Walt,et al.  Optical fiber-based biosensors , 2004, Analytical and bioanalytical chemistry.

[10]  Longhua Tang,et al.  Graphene oxide amplified electrogenerated chemiluminescence of quantum dots and its selective sensing for glutathione from thiol-containing compounds. , 2009, Analytical chemistry.

[11]  G. Rivas,et al.  DNA-Modified Electrode for the Detection of Aromatic Amines , 1996 .

[12]  Joseph Wang,et al.  Ultrasensitive electrical biosensing of proteins and DNA: carbon-nanotube derived amplification of the recognition and transduction events. , 2004, Journal of the American Chemical Society.

[13]  Chunhai Fan,et al.  Graphene oxide-facilitated electron transfer of metalloproteins at electrode surfaces. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[14]  Ronen Polsky,et al.  Genomagnetic electrochemical assays of DNA hybridization. , 2002, Talanta.

[15]  Chun Li,et al.  Chemically converted graphene induced molecular flattening of 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin and its application for optical detection of cadmium(II) ions. , 2009, Journal of the American Chemical Society.

[16]  Ying Wang,et al.  Application of graphene-modified electrode for selective detection of dopamine , 2009 .

[17]  E. Wang,et al.  Nafion–graphene nanocomposite film as enhanced sensing platform for ultrasensitive determination of cadmium , 2009 .

[18]  Giovanna Marrazza,et al.  Electrochemical and piezoelectric DNA biosensors for hybridisation detection. , 2008, Analytica chimica acta.

[19]  David R Walt,et al.  Very high density sensing arrays. , 2008, Chemical reviews.

[20]  G Marrazza,et al.  Electrochemical DNA biosensor for analysis of wastewater samples. , 2002, Bioelectrochemistry.

[21]  Zhong Lin Wang,et al.  DNA functionalized single-walled carbon nanotubes for electrochemical detection. , 2005, The journal of physical chemistry. B.

[22]  Giovanna Marrazza,et al.  Disposable DNA electrochemical biosensors for environmental monitoring , 1999 .

[23]  Michael T. Carter,et al.  Voltammetric studies of the interaction of metal chelates with DNA. 2. Tris-chelated complexes of cobalt(III) and iron(II) with 1,10-phenanthroline and 2,2'-bipyridine , 1989 .

[24]  Giovanna Marrazza,et al.  Electrochemical DNA biosensor for environmental monitoring , 2001 .

[25]  A. Baeumner,et al.  Highly sensitive and specific detection of viable Escherichia coli in drinking water. , 2002, Analytical biochemistry.

[26]  Yongsheng Chen,et al.  DNA electrochemical sensor based on an adduct of single-walled carbon nanotubes and ferrocene , 2007, Biotechnology Letters.

[27]  Andre K. Geim,et al.  The rise of graphene. , 2007, Nature materials.

[28]  P. Stoddart,et al.  Nanostructured optical fibre arrays for high-density biochemical sensing and remote imaging , 2010, Analytical and bioanalytical chemistry.

[29]  M. Meyyappan,et al.  Carbon Nanotube Nanoelectrode Array for Ultrasensitive DNA Detection , 2003 .

[30]  Lifeng Yan,et al.  Preparation of graphene by the rapid and mild thermal reduction of graphene oxide induced by microwaves , 2010 .

[31]  K. Loh,et al.  Direct voltammetric detection of DNA and pH sensing on epitaxial graphene: an insight into the role of oxygenated defects. , 2010, Analytical chemistry.

[32]  Ke-Jing Huang,et al.  Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA. , 2011, Colloids and surfaces. B, Biointerfaces.

[33]  E. Paleček,et al.  Interactions of antitumor drug daunomycin with DNA in solution and at the surface , 1998 .

[34]  P. J. Ollivier,et al.  Layer-by-Layer Assembly of Ultrathin Composite Films from Micron-Sized Graphite Oxide Sheets and Polycations , 1999 .

[35]  K. Hashimoto,et al.  Sequence-specific gene detection with a gold electrode modified with DNA probes and an electrochemically active dye. , 1994, Analytical chemistry.

[36]  Zhuo. Sun,et al.  Capacitive behavior of graphene–ZnO composite film for supercapacitors , 2009 .