DIAPH 3 governs the cellular transition to the amoeboid tumor phenotype

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[1]  L. Blanchoin,et al.  Differential interactions of the formins INF2, mDia1, and mDia2 with microtubules , 2011, Molecular biology of the cell.

[2]  Z. Kam,et al.  Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics , 2011, Molecular biology of the cell.

[3]  Y. Mao,et al.  Aurora B regulates formin mDia3 in achieving metaphase chromosome alignment. , 2011, Developmental cell.

[4]  B. Szabo,et al.  TPPP/p25 Promotes Tubulin Acetylation by Inhibiting Histone Deacetylase 6* , 2010, The Journal of Biological Chemistry.

[5]  T. Yao,et al.  The Microtubule-associated Histone Deacetylase 6 (HDAC6) Regulates Epidermal Growth Factor Receptor (EGFR) Endocytic Trafficking and Degradation* , 2010, The Journal of Biological Chemistry.

[6]  G. Gundersen,et al.  Formins and microtubules. , 2010, Biochimica et biophysica acta.

[7]  Melissa A. Chesarone,et al.  Unleashing formins to remodel the actin and microtubule cytoskeletons , 2010, Nature Reviews Molecular Cell Biology.

[8]  E. Perez.  Microtubule inhibitors: Differentiating tubulin-inhibiting agents based on mechanisms of action, clinical activity, and resistance , 2009, Molecular Cancer Therapeutics.

[9]  John D. Minna,et al.  Molecular Profiling of Breast Cancer Cell Lines Defines Relevant Tumor Models and Provides a Resource for Cancer Gene Discovery , 2009, PloS one.

[10]  M. Rubin,et al.  Oncosome formation in prostate cancer: association with a region of frequent chromosomal deletion in metastatic disease. , 2009, Cancer research.

[11]  Elaine Fuchs,et al.  Cyfip1 Is a Putative Invasion Suppressor in Epithelial Cancers , 2009, Cell.

[12]  F. Khuri,et al.  Quantum dot-based quantification revealed differences in subcellular localization of EGFR and E-cadherin between EGFR-TKI sensitive and insensitive cancer cells , 2009, Nanotechnology.

[13]  Guillem Rigaill,et al.  Diaphanous-related formins are required for invadopodia formation and invasion of breast tumor cells. , 2009, Cancer research.

[14]  J. Moseley,et al.  The formin mDia2 stabilizes microtubules independently of its actin nucleation activity , 2008, The Journal of cell biology.

[15]  M. Ono,et al.  The EGFR inhibitor gefitinib suppresses ligand-stimulated endocytosis of EGFR via the early/late endocytic pathway in non-small cell lung cancer cell lines , 2007, Histochemistry and Cell Biology.

[16]  J. Resau,et al.  RhoB and the mammalian Diaphanous-related formin mDia2 in endosome trafficking. , 2007, Experimental cell research.

[17]  G. Gundersen,et al.  The formin mDia regulates GSK3beta through novel PKCs to promote microtubule stabilization but not MTOC reorientation in migrating fibroblasts. , 2006, Molecular biology of the cell.

[18]  M. Schliwa,et al.  Powering membrane traffic in endocytosis and recycling , 2006, Nature Reviews Molecular Cell Biology.

[19]  D. Lauffenburger,et al.  Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[20]  J. Neefjes,et al.  RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1 , 2005, Journal of Cell Science.

[21]  S. Hamm-Alvarez,et al.  Paclitaxel and Nocodazole Differentially Alter Endocytosis in Cultured Cells , 1996, Pharmaceutical Research.

[22]  Jun Peng,et al.  Disruption of the Diaphanous-Related Formin Drf1 Gene Encoding mDia1 Reveals a Role for Drf3 as an Effector for Cdc42 , 2003, Current Biology.

[23]  Y. Kalaidzidis,et al.  RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase , 2003, Nature Cell Biology.

[24]  Peter Friedl,et al.  Compensation mechanism in tumor cell migration , 2003, The Journal of cell biology.

[25]  Jayanta Debnath,et al.  The Role of Apoptosis in Creating and Maintaining Luminal Space within Normal and Oncogene-Expressing Mammary Acini , 2002, Cell.

[26]  E. Turley,et al.  Active erk regulates microtubule stability in H-ras-transformed cells. , 2001, Neoplasia.

[27]  K. Sass,et al.  N-Cadherin Extracellular Repeat 4 Mediates Epithelial to Mesenchymal Transition and Increased Motility , 2000, The Journal of cell biology.

[28]  H. Frierson,et al.  Activation of mitogen-activated protein kinase associated with prostate cancer progression. , 1999, Cancer research.

[29]  E. Barrón,et al.  Taxol inhibits endosomal-lysosomal membrane trafficking at two distinct steps in CV-1 cells. , 1998, The American journal of physiology.