Evaluation of monoclonal antibody based immunochromatographic strip test for direct detection of Vibrio cholerae O1 contamination in seafood samples.

A strip test for the detection of Vibrio cholerae O1 was developed using two monoclonal antibodies (MAbs), VC-223 and VC-1226, specific to the lipopolysaccharides of Vibrio cholerae O1 Inaba and Ogawa serovars. The sensitivity of the test was 5 × 10(5)cfu/mL which was similar to that of dot blot test. The detection limit could be improved to 1cfu/mL of the original bacterial content after pre-incubation of the bacterium in alkaline peptone water (APW) for 12h. Detection of V. cholerae O1 in various fresh seafood samples such as shrimp, blood clam, mussel and oyster could be performed directly with sensitivities ranged from 5 × 10(5) to 10(6)cfu/mL. After pre-enrichment of the shrimp sample in APW, the detection sensitivities increased to 10(2) to 10CFU/mL of the original bacterial content after incubation for 12 and 24h. However, the detection sensitivities were also depending on the content of the other bacteria that might inhibit the growth of V. cholerae during pre-enrichment step. The V. cholerae O1 strip test has advantages in speed, and simplicity in not requiring sophisticated equipment or specialized skills and the sample could be directly examined without requirement for sample processing.

[1]  R. Colwell,et al.  ompU genes in non‐toxigenic Vibrio cholerae associated with aquaculture , 2003, Journal of applied microbiology.

[2]  R. Colwell,et al.  Development of a Hexaplex PCR Assay for Rapid Detection of Virulence and Regulatory Genes in Vibrio cholerae and Vibrio mimicus , 2002, Journal of Clinical Microbiology.

[3]  A. C. Ghose,et al.  Rapid Method for Species-Specific Identification ofVibrio cholerae Using Primers Targeted to the Gene of Outer Membrane Protein OmpW , 2000, Journal of Clinical Microbiology.

[4]  Parin Chaivisuthangkura,et al.  Development of monoclonal antibodies for simple detection and differentiation of Vibrio mimicus from V. cholerae and Vibrio spp. by dot blotting , 2010 .

[5]  M. Henk,et al.  Detection of Vibrio cholerae with monoclonal antibodies specific for serovar O1 lipopolysaccharide , 1988, Journal of clinical microbiology.

[6]  B. Fraser.  Haiti still gripped by cholera as election looms , 2010, The Lancet.

[7]  R. Sack,et al.  Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples , 1995, Journal of clinical microbiology.

[8]  J. Kirigia,et al.  Economic burden of cholera in the WHO African region , 2009 .

[9]  K. Chaudhuri,et al.  Lipopolysaccharides of Vibrio cholerae. I. Physical and chemical characterization. , 2003, Biochimica et biophysica acta.

[10]  Parin Chaivisuthangkura,et al.  A convenient immunochromatographic test strip for rapid diagnosis of yellow head virus infection in shrimp. , 2007, Journal of virological methods.

[11]  S K Bhattacharya,et al.  Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139. , 1998, FEMS immunology and medical microbiology.

[12]  C. Yu,et al.  Molecular Evidence of Cholera Outbreak Caused by a Toxigenic Vibrio cholerae O1 El Tor Variant Strain in Kelantan, Malaysia , 2010, Journal of Clinical Microbiology.

[13]  T. Yokota,et al.  Different types of monoclonal antibodies to Ogawa-specific and group-specific antigens of Vibrio cholerae O1 , 1987, Journal of clinical microbiology.

[14]  G. Nair,et al.  Rapid Diagnosis of Cholera Caused by Vibrio cholerae O139 , 1998, Journal of Clinical Microbiology.

[15]  T. Ramamurthy,et al.  PCR-based identification of Vibrio cholerae and the closely related species Vibrio mimicus using the large chromosomal ori sequence of Vibrio cholerae. , 2006, FEMS microbiology letters.

[16]  A. Koumaré,et al.  Evaluation of immunization coverage within the Expanded Program on Immunization in Kita Circle, Mali: a cross-sectional survey , 2009, BMC international health and human rights.

[17]  D. Sack,et al.  One-Step Immunochromatographic Dipstick Tests for Rapid Detection of Vibrio cholerae O1 and O139 in Stool Samples , 2003, Clinical Diagnostic Laboratory Immunology.

[18]  D. Ottaviani,et al.  Prevalence and virulence properties of non-O1 non-O139 Vibrio cholerae strains from seafood and clinical samples collected in Italy. , 2009, International journal of food microbiology.

[19]  S. H. Lee,et al.  Development of rapid one-step immunochromatographic assay. , 2000, Methods.

[20]  Parin Chaivisuthangkura,et al.  Rapid and sensitive detection of Vibrio cholerae by loop‐mediated isothermal amplification targeted to the gene of outer membrane protein ompW , 2010, Letters in applied microbiology.

[21]  M. Cohen,et al.  A human volunteer challenge model using frozen bacteria of the new epidemic serotype, V. cholerae O139 in Thai volunteers. , 2001, Vaccine.

[22]  R. Colwell,et al.  A novel kit for rapid detection of Vibrio cholerae O1 , 1994, Journal of clinical microbiology.

[23]  S. Venter,et al.  Detection of toxigenic Vibrio cholerae from environmental water samples by an enrichment broth cultivation–pit‐stop semi‐nested PCR procedure , 2000, Journal of applied microbiology.

[24]  Parin Chaivisuthangkura,et al.  A simple and rapid immunochromatographic test strip for detection of white spot syndrome virus (WSSV) of shrimp. , 2006, Diseases of aquatic organisms.

[25]  T. Yokota,et al.  Monoclonal antibodies to Vibrio cholerae O1 serotype inaba , 1988, Journal of clinical microbiology.

[26]  Y. Takeda,et al.  Evaluation of a rapid immunochromatographic dipstick kit for diagnosis of cholera emphasizes its outbreak utility. , 2010, Japanese journal of infectious diseases.

[27]  T. Ezaki,et al.  Rapid and specific identification of 5 human pathogenic Vibrio species by multiplex polymerase chain reaction targeted to dnaJ gene. , 2007, Diagnostic microbiology and infectious disease.

[28]  M. López-Sabater,et al.  Shelf-Life Prediction of an Infant Formula Using an Accelerated Stability Test (Rancimat) , 1995 .

[29]  Paula M. Imbro,et al.  Use of a real time PCR assay for detection of the ctxA gene of Vibrio cholerae in an environmental survey of Mobile Bay. , 2007, Journal of microbiological methods.

[30]  N. Sithivong,et al.  A cholera epidemic in Sekong Province, Lao People's Democratic Republic, December 2007-January 2008. , 2010, Japanese journal of infectious diseases (Print).

[31]  Parin Chaivisuthangkura,et al.  Simultaneous and rapid detection of white spot syndrome virus and yellow head virus infection in shrimp with a dual immunochromatographic strip test. , 2011, Journal of virological methods.

[32]  R. Morona,et al.  Serotype conversion in Vibrio cholerae O1. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[33]  S. Lukinmaa,et al.  Territorial waters of the Baltic Sea as a source of infections caused by Vibrio cholerae non-O1, non-O139: report of 3 hospitalized cases. , 2006, Diagnostic microbiology and infectious disease.

[34]  F. Siddiqui,et al.  Consecutive outbreaks of Vibrio cholerae O139 and V. cholerae O1 cholera in a fishing village near Karachi, Pakistan. , 2006, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[35]  R. Sack,et al.  Development and evaluation of rapid monoclonal antibody-based coagglutination test for direct detection of Vibrio cholerae O139 synonym Bengal in stool samples , 1994, Journal of clinical microbiology.

[36]  Parin Chaivisuthangkura,et al.  Differentiation among the Vibrio cholerae serotypes O1, O139, O141 and non-O1, non-O139, non-O141 using specific monoclonal antibodies with dot blotting. , 2011, Journal of microbiological methods.