The ultrastructure of capillary sprouts induced by melanoma transplants in the golden hamster.

S Y N O P S I S Capillary sprouts were induced by the transplantation of fragments of melanoma onto the surface of the cheek pouch of the golden hamster. By means of a transparent chamber technique the growth of capillaries into the transplanted tumour tissue was observed in vivo, usually within three days, and it was possible to locate suitable specimens for study by electron microscopy. The rapidity of the process of vascularization of the tumour fragments indicates that the stimulus to new capillary formation is considerable. The morphology of the capillary sprouts induced by these transplants included those features of greatly dilated endoplasmic reticulum, many ribosomes, numerous mitochondria, and prominent Golgi units which are features of cells actively synthesising protein (De Robertis & De Iraldi, 1964). Numerous microvilli were present on the luminal surface of the endothzlium of vessels close to the transplant and these cells often contained multivesicular bodies and centrioles. The increase in organelles found in the cells of the newly formed capillaries was similar to, though possibly more marked than, that noted in the capillaries of healing tissue (Cliff, 1963; Schoefl, 1963).

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