A Critical Survey of Current Approaches in Quantitative Histo- and Cytochemistry*

Publisher Summary This chapter discusses current approaches and several fundamental issues in quantitative histo- and cytochemistry. Visible, ultraviolet, and X-ray absorption studies are all brought to bear on the problem with telling effect. The greater the degree of integration of experimental approaches, the greater the ultimate advance. The chapter discusses the quantitative chemical analysis of the cell, its parts and extracellular products, in situ , and in the living state. Studies on living material that are made on several single-cell organisms and certain large plant cells are presented. Preservation of the original morphology and chemical constitution in nonliving material is advanced, and means for reliable analyses are developed. The techniques for direct chemical analysis of smaller samples, down to the level of small cells and even their intracellular components, and the attainment of higher resolution in analysis by X-ray absorption and autoradiography are discussed.

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