Oxidation kinetics and chemostat growth kinetics of Thiobacillus ferrooxidans on tetrathionate and thiosulfate

Growth of Thiobacillus ferrooxidans in batch culture on 10 mM potassium tetrathionate was optimal at pH 2.5 (specific growth rate, 0.092 h-1). Oxygen electrode studies on resting cell suspensions showed that the apparent Km for tetrathionate oxidation (0.13 to 8.33 mM) was pH dependent, suggesting higher substrate affinity at higher pH. Conversely, oxidation rates were greatest at low pH. High substrate concentrations (7.7 to 77 mM) did not affect maximum oxidation rates at pH 3.0, but produced substrate inhibition at other pH values. Tetrathionate-grown cell suspensions also oxidized thiosulfate at pH 2.0 to 4.0. Apparent Km values (1.2 to 25 mM) were of the same order as for tetrathionate, but kinetics were complex. Continuous culture on growth-limiting tetrathionate at pH 2.5, followed by continuous culture on growth-limiting thiosulfate at pH 2.5, indicated true growth yield values (grams [dry weight] per gram-molecule of substrate) of 12.2 and 7.5, and maintenance coefficient values (millimoles of substrate per gram [dry weight) of organisms per hour) of 1.01 and 0.97 for tetrathionate and thiosulfate, respectively. Yield was increased on both media at low dilution rates by increase in CO2 supply. The apparent maintenance coefficient was lowered without affecting YG, suggesting better energy coupling in CO2-rich environments. Prolonged continuous cultivation on tetrathionate or thiosulfate did not affect the ability of the organism to grow subsequently in ferrous iron medium.

[1]  D. Kelly,et al.  Growth Kinetics of Thiobacillus denitrificans in Anaerobic and Aerobic Chemostat Culture , 1978 .

[2]  C. Jones,et al.  FACTORS AFFECTING METABOLISM AND FERROUS IRON OXIDATION IN SUSPENSIONS AND BATCH CULTURES OF THIOBACILLUS FERROOXIDANS: RELEVANCE TO FERRIC IRON LEACH SOLUTION REGENERATION , 1978 .

[3]  O. Tuovinen,et al.  Recommendation that the Names Ferrobacillus ferrooxidans Leathen and Braley and Ferrobacillus sulfooxidans Kinsel Be Recognized as Synonyms of Thiobacillus ferrooxidans Temple and Colmer , 1972 .

[4]  A. Colmer,et al.  Comparison of the kinetics of thiosulfate oxidation by three iron-sulfur oxidizers. , 1972, Canadian journal of microbiology.

[5]  O. Tuovinen,et al.  Biology of Thiobacillus ferrooxidans in relation to the microbiological leaching of sulphide ores. , 1972, Zeitschrift fur allgemeine Mikrobiologie.

[6]  D. Walker,et al.  The generation and utilization of energy during growth. , 1971, Advances in microbial physiology.

[7]  D. P. Kelly,et al.  Cyanolysis and spectrophotometric estimation of trithionate in mixture with thiosulfate and tetrathionate , 1969 .

[8]  H. D. Peck Energy-coupling mechanisms in chemolithotrophic bacteria. , 1968, Annual review of microbiology.

[9]  W. Hempfling,et al.  Yield Coefficients of Thiobacillus neapolitanus in Continuous Culture , 1967, Journal of bacteriology.

[10]  D. Lundgren,et al.  Sulfur Oxidation by the Iron Bacterium Ferrobacillus ferrooxidans , 1966, Journal of bacteriology.

[11]  D. W. Duncan,et al.  Oxidation of inorganic sulfur compounds by washed cell suspensions of Thiobacillus ferrooxidans. , 1966, Canadian journal of microbiology.

[12]  D. White,et al.  Taxonomy of the acidophilic thiobacilli. , 1966, Journal of general microbiology.

[13]  M. Silver,et al.  The initial product and properties of the sulfur-oxidizing enzyme of thiobacilli. , 1966, Biochimica et biophysica acta.

[14]  D. P. Kelly,et al.  Energy coupling during sulphur compound oxidation by Thiobacillus sp. strain C. , 1966, Journal of general microbiology.

[15]  D. P. Kelly,et al.  Effect of 2:4-Dinitrophenol on Carbon Dioxide Fixation by a Thiobacillus , 1963, Nature.

[16]  A. Colmer,et al.  RELATION OF THE IRON OXIDIZER, THIOBACILLUS FERROOXIDANS, TO THIOSULFATE , 1962, Journal of bacteriology.

[17]  R. F. Unz,et al.  A COMPARATIVE NUTRITIONAL STUDY OF THREE CHEMOAUTOTROPHIC BACTERIA: FERROBACILLUS FERROOXIDANS, THIOBACILLUS FERROOXIDANS AND THIOBACILLUS THIOOXIDANS , 1961 .

[18]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[19]  K. Temple,et al.  THE AUTOTROPHIC OXIDATION OF IRON BY A NEW BACTERIUM: THIOBACILLUS FERROOXIDANS , 1951, Journal of bacteriology.

[20]  K. Temple,et al.  AN IRON-OXIDIZING BACTERIUM FROM THE ACID DRAINAGE OF SOME BITUMINOUS COAL MINES , 1950, Journal of bacteriology.