Light scattering of normal human lens I. Application of random density and orientation fluctuation theory.

Light-scattering intensities in the I parallel and I+ mode were obtained on thin sections of three human lenses. Random density and orientation fluctuation theory, without cross correlation, was employed to evaluate light-scattering parameters. Both the density correlation distances, as well as the orientation correlation distances, were related to structural elements in the lens fiber cell that have been observed by other investigators with different techniques. The magnitude of these fluctuations were evaluated, and it was demonstrated that the density fluctuations are the main contributors to light scattering in normal human lenses. Changes in the light-scattering parameters were evaluated as a function of position within the lens. The changes observed agree with the biochemical data in the literature that reflects that an aging process occurs when one proceeds from the periphery of the lens toward the center.

[1]  B. Philipson.  Changes in the lens related to the reduction of transparency. , 1973, Experimental eye research.

[2]  H. Hoenders,et al.  On the quaternary structure of high-molecular-weight proteins from the bovine eye lens. , 1975, European journal of biochemistry.

[3]  F. Bettelheim,et al.  Light‐scattering studies of crosslinking unsaturated polyesters with methyl acrylate , 1962 .

[4]  R. Stein.  The determination of the inhomogeneity of crosslinking of a rubber by light scattering , 1969 .

[5]  M. Perry,et al.  Electron microscope observations on some structural proteins of the chick lens. , 1972, Experimental eye research.

[6]  A. Spector,et al.  Age-dependent changes in the molecular size of human lens proteins and their relationship to light scatter. , 1974, Investigative ophthalmology.

[7]  G. Benedek,et al.  Theory of transparency of the eye. , 1971, Applied optics.

[8]  S. Trokel.  The physical basis for transparency of the crystalline lens. , 1962, Investigative ophthalmology.

[9]  R. Stein,et al.  The scattering of light from thin polymer films. I. Experimental procedure , 1955 .

[10]  L. Chylack,et al.  Studies on human cataracts. II. Correlation between the clinical description and the light-scattering parameters of human cataracts. , 1981, Investigative ophthalmology & visual science.

[11]  T. Wanko,et al.  Electron Microscope Study of Lens Fibers , 1959, The Journal of biophysical and biochemical cytology.

[12]  F. Bettelheim.  Induced optical anisotropy fluctuation in the lens of the eye , 1978 .

[13]  N. Rafferty,et al.  Cytoplasmic filaments in the crystalline lens of various species: functional correlations. , 1978, Experimental eye research.

[14]  B. Philipson,et al.  Distribution of protein within the normal rat lens. , 1969, Investigative ophthalmology.

[15]  F. Bettelheim,et al.  Small-angle light scattering studies on xylose cataract formation in bovine lenses. , 1978, Investigative Ophthalmology and Visual Science.

[16]  A. Spector,et al.  Age-dependent changes in the structure of alpha crystallin. , 1971, Investigative ophthalmology.

[17]  O. Kratky.  Possibilities of X-ray small angle analysis in the investigation of dissolved and solid high polymer substances , 1966 .

[18]  F. Bettelheim,et al.  SOME LIGHT‐SCATTERING STUDIES OF THE TEXTURE OF CRYSTALLINE POLYMERS * , 1959 .

[19]  F. Bettelheim.  Syneresis and its possible role in cataractogenesis. , 1979, Experimental eye research.

[20]  E. Balazs,et al.  Biochemical and fine structure studies on the water-insoluble components of the calf lens. , 1972, Experimental eye research.

[21]  F. Bettelheim.  On the optical anisotropy of lens fiber cells. , 1975, Experimental eye research.

[22]  A. M. Bueche,et al.  Scattering by an Inhomogeneous Solid , 1949 .

[23]  M. Vinciguerra,et al.  LASER‐DIFFRACTION PATTERNS OF HIGHLY ORDERED SUPERSTRUCTURES IN THE LENSES OF BOVINE EYES , 1971, Annals of the New York Academy of Sciences.