The 70‐kDa major DNA‐compacting protein of the chloroplast nucleoid is sulfite reductase

The chloroplast nucleoid is a complex of chloroplast DNA and various, mostly uncharacterized proteins. An abundant 70‐kDa protein of the isolated nucleoids of pea chloroplasts was identified as sulfite reductase by N‐terminal sequence analysis as well as immunoblot analysis, spectrophotometry and enzyme activity analysis. Recombinant maize sulfite reductase was indeed able to compact chloroplast DNA and to form nucleoid‐like particles in vitro. The role of sulfite reductase in the structural organization of the nucleoid is discussed.

[1]  T. Kuroiwa,et al.  Detection and characterization of a plastid envelope DNA‐binding protein which may anchor plastid nucleoids. , 1993, The EMBO journal.

[2]  T. Kuroiwa,et al.  Studies on plastid-nuclei (nucleoids) in Nicotiana tabacum L. III. Isolation of chloroplast nuclei from mesophyll protoplasts and identification of chloroplast DNA-binding proteins. , 1990 .

[3]  J. Marrison,et al.  Co‐immunolocalization of topoisomerase II and chloroplast DNA in developing, dividing and mature wheat chloroplasts , 1992 .

[4]  John Moncrieff,et al.  Photosynthesis: from Light to Biosphere , 1995 .

[5]  M. Block,et al.  Do plastid envelope membranes play a role in the expression of the plastid genome? , 1999, Biochimie.

[6]  T. Ashikari,et al.  Molecular characterization of tobacco sulfite reductase: enzyme purification, gene cloning, and gene expression analysis. , 1998, Journal of biochemistry.

[7]  T. Kuroiwa,et al.  Studies on Plastid-Nuclei (Nucleoids) in Nicotiana tabacum L. I. Isolation of Proplastid-Nuclei from Cultured Cells and Identification of Proplastid-Nuclear Proteins , 1988 .

[8]  Transcriptional Activity of Morphologically Intact Proplastid-Nuclei (Nucleoids) Isolated from Tobacco Cultured Cells , 1991 .

[9]  T. Kuroiwa.  The replication, differentiation, and inheritance of plastids with emphasis on the concept of organelle nuclei , 1991 .

[10]  A. Nerozzi,et al.  Localization of plastid DNA replication on a nucleoid structure. , 1997, American journal of botany.

[11]  F. Sato,et al.  A novel protein with DNA binding activity from tobacco chloroplast nucleoids. , 1997, The Plant cell.

[12]  S. Clark,et al.  High sensitivity mapping of methylated cytosines. , 1994, Nucleic acids research.

[13]  C. Brunold,et al.  Measurement of ferredoxin-dependent sulfite reductase activity in crude extracts from leaves using O-acetyl-L-serine sulfhydrylase in a coupled assay system to measure the sulfide formed. , 1983, Analytical biochemistry.

[14]  N. Sato,et al.  Molecular Characterization of the PEND Protein, a Novel bZIP Protein Present in the Envelope Membrane That Is the Site of Nucleoid Replication in Developing Plastids , 1998, Plant Cell.