Effect of insulin-like growth factor-1 and hyaluronic acid in experimentally produced osteochondral defects in rats

Background The common purpose of almost all methods used to treat the osteochondral injuries is to produce a normal cartilage matrix. However current methods are not suffcient to provide a normal cartilage matrix. For that reason, researchers have studied to increase the effectiveness of this methods using chondrogenic and chondroprotective molecules in recent experimental studies. Insulin-like growth factor-1 (IGF-1) and hyaluronic acid (HA) are two important agents used in this feld. This study compared the effects of IGF-1 and HA in an experimental osteochondral defect in rat femora. Materials and Methods The rats were divided into three groups ( n = 15 per group) as follows: The IGF-1 group, HA group, and control group. An osteochondral defect of a diameter of 1.5 mm and a depth of 2 mm was created on the patellar joint side of femoral condyles. The IGF-1 group received an absorbable gelatin sponge soaked with 15 μg/15 μl of IGF-1, and the HA group received an absorbable gelatin sponge soaked with 80 μg HA. The control group received only an absorbable gelatin sponge. Rats were sacrifced at the 6th week, and the femur condyles were evaluated histologically. Results According to the total Mankin scale, there was a statistically signifcant difference between IGF-1 and HA groups and between IGF-1 and control groups. There was also a signifcant statistical difference between HA and control groups. Conclusion It was shown histopathologically that IGF-1 is an effective molecule for osteochondral lesions. Although it is weaker than IGF-1, HA also strengthened the repair tissue.

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