Electrochemical impedance detection of DNA hybridization based on dendrimer modified electrode.

Bioactive ultrathin films with the incorporation of amino-terminated G4 PAMAM dendrimers have been prepared via layer-by-layer self-assembly methods on a gold electrode and used for the DNA hybridization analysis. Surface plasmon resonance (SPR), X-ray photoelectron spectroscopy (XPS), and electrochemical impedance spectroscopy (EIS) are used to characterize the successful construction of the multicomponent film on the gold substrate. The dendrimer-modified surfaces improve the immobilization capacity of the probe DNA greatly, compared to the AET (2-aminoethanethiol) SAM sensor surfaces without dendrimer molecules. DNA hybridization analysis is monitored by EIS. The dendrimer-based electrochemical impedance DNA biosensor shows high sensitivity and selectivity for DNA hybridization assay. The multicomponent films also display a high stability during repeated regeneration and hybridization cycles.

[1]  I. Willner,et al.  Electronic transduction of DNA sensing processes on surfaces: amplification of DNA detection and analysis of single-base mismatches by tagged liposomes. , 2001, Journal of the American Chemical Society.

[2]  Wei Sun,et al.  An Electrochemical DNA Sensor Based on Electrodepositing Aluminum Ion Films on Stearic Acid‐Modified Carbon Paste Electrode and Its Application for the Detection of Specific Sequences Related to Bar Gene and CP4 Epsps Gene , 2005 .

[3]  Z. Cheng,et al.  Capacitive detection of glucose using molecularly imprinted polymers. , 2001, Biosensors & bioelectronics.

[4]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[5]  T. K. Christopoulos,et al.  Enzyme-amplified aequorin-based bioluminometric hybridization assays. , 2001, Analytical chemistry.

[6]  O. Sadik,et al.  Enzyme-modulated cleavage of dsDNA for supramolecular design of biosensors. , 2001, Analytical chemistry.

[7]  H. Yoon,et al.  Reversible Association/Dissociation Reaction of Avidin on the Dendrimer Monolayer Functionalized with a Biotin Analogue for a Regenerable Affinity-Sensing Surface , 2001 .

[8]  Joel S. Silverman,et al.  Detection of Attomole Quantitites of DNA Targets on Gold Microelectrodes by Electrocatalytic Nucleobase Oxidation , 2003 .

[9]  Joseph Wang,et al.  Metal nanoparticle-based electrochemical stripping potentiometric detection of DNA hybridization. , 2001, Analytical chemistry.

[10]  Shanlin Pan,et al.  Chemical control of electrode functionalization for detection of DNA hybridization by electrochemical impedance spectroscopy. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[11]  P. He,et al.  Probing DNA Hybridization by Impedance Measurement Based on CdS‐Oligonucleotide Nanoconjugates , 2004 .

[12]  J. Dougherty,et al.  Rapid hybridization kinetics of DNA attached to submicron latex particles. , 1987, Nucleic acids research.

[13]  S Tombelli,et al.  Biosensors as new analytical tool for detection of Genetically Modified Organisms (GMOs) , 2001, Fresenius' journal of analytical chemistry.

[14]  G. Rivas,et al.  Electrochemical Biosensors for Sequence‐Specific DNA Detection , 2005 .

[15]  C. Mirkin,et al.  Scanometric DNA array detection with nanoparticle probes. , 2000, Science.

[16]  Adam Heller,et al.  Screen printing of nucleic acid detecting carbon electrodes. , 2002, Analytical chemistry.

[17]  H. S. Kim,et al.  Affinity biosensor for avidin using a double functionalized dendrimer monolayer on a gold electrode. , 2000, Analytical biochemistry.

[18]  Chen-Zhong Li,et al.  Impedance sensing of DNA binding drugs using gold substrates modified with gold nanoparticles. , 2005, Analytical chemistry.

[19]  Kim,et al.  Multilayered assembly of dendrimers with enzymes on gold: thickness-controlled biosensing interface , 2000, Analytical chemistry.

[20]  Yanbin Li,et al.  Immunobiosensor chips for detection of Escherichia coil O157:H7 using electrochemical impedance spectroscopy. , 2002, Analytical chemistry.

[21]  H. Xie,et al.  Amperometric detection of nucleic acid at femtomolar levels with a nucleic acid/electrochemical activator bilayer on gold electrode. , 2004, Analytical chemistry.

[22]  Long Jiang,et al.  Enhancement of DNA immobilization and hybridization on gold electrode modified by nanogold aggregates. , 2005, Biosensors & bioelectronics.

[23]  Christian Soeller,et al.  Electrochemical detection of DNA hybridization amplified by nanoparticles. , 2006, Biosensors & bioelectronics.

[24]  Carl A. Batt,et al.  Dendrimer-functionalized self-assembled monolayers as a surface plasmon resonance sensor surface. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[25]  C. Brett,et al.  Adsorption of Guanine, Guanosine, and Adenine at Electrodes Studied by Differential Pulse Voltammetry and Electrochemical Impedance , 2002 .

[26]  Elizabeth M. Boon,et al.  Mutation detection by electrocatalysis at DNA-modified electrodes , 2000, Nature Biotechnology.

[27]  J. Rusling,et al.  Square wave voltammetric detection of chemical DNA damage with catalytic poly(4-vinylpyridine)-Ru(bpy)2(2+) films. , 2002, Analytical chemistry.

[28]  Itamar Willner,et al.  Amplified DNA detection by electrogenerated biochemiluminescence and by the catalyzed precipitation of an insoluble product on electrodes in the presence of the doxorubicin intercalator. , 2002, Angewandte Chemie.

[29]  Serge Cosnier,et al.  Affinity Biosensors Based on Electropolymerized Films , 2005 .