论文信息 - TheActin-related Protein Act3pofSaccharomyces cerevisiae IsLocated intheNucleus

TheActin-related Protein Act3pofSaccharomyces cerevisiae IsLocated intheNucleus

Actin-related proteins, agroupofprotein families thatexhibit about50%sequence identity amongeachother andtoconventional actin, havebeenfoundinavariety of eukaryotic organisms. Inthebudding yeast Saccharomyces cerevisiae, genesforoneconventional actin (ACTI) andforthree actin-related proteins (ACT2, ACT3,andACT5)are known.ACT3,whichwerecently discovered, isanessential genecoding forapolypeptide of489aminoacids (Act3p), withacalculated molecular massof54.8 kDa.Besides its homology toconventional actin, Act3ppossesses adomainexhibiting weaksimilarity to thechromosomal protein HMG-14aswellasapotential nuclear localization signal. An antiserum prepared against aspecific segment oftheACT3geneproduct recognizes a polypeptide bandofapproximately 55kDainyeast extract. Indirect immunofluorescence experiments withthis antiserum revealed that Act3pislocated inthenucleus. Nuclear staining wasobserved inallcells regardless ofthestage ofthecell cyle. Independently, immunoblotting experiments withsubcellular fractions showedthatAct3pisindeed highly enriched inthenuclear fraction. We suggest that Act3pisanessential constituent ofyeast chromatin.

M. Harata | V. Weber

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