FTIR Characterization and Release of Bovine Serum Albumin from Bioactive Glasses

Background Bioactive glass has attracted substantial interest in orthopedics, but it has been less explored as a drug carrier. This study investigated the bovine serum albumin (BSA) release from bioactive 13–93B0 and 13–93B3 glasses. Methods Glass disks (13–93B0 and 13–93B3; n = 5) were loaded with 4 mg of BSA and coated under different chitosan-coating conditions. The amount of BSA released in phosphate-buffered saline (PBS) was evaluated, and a degradation study was performed to find out the weight loss and pH of PBS. Secondary structures of BSA on 13–93B0 were characterized by Fourier transform infrared (FTIR) spectroscopy. Results One hundred percent protein release occurred by 24 hours for all 13–93B3 groups. However, chitosan coating delayed 100% release up to 72 hours in 13–93B0 groups. The 13–93B3 glass showed higher degradation rates than 13–93B0 regardless of chitosan-coating status. Multilayer and sandwich chitosan coatings further delayed BSA release from 13–93B0. FTIR analysis revealed that α-helical structure was the highest among all groups and significantly higher in the 2% sandwich chitosan coating group (32.0% ± 2.1%), compared with uncoated and 4% chitosan groups. Conclusions Chitosan coating can delay the burst release of BSA from 13–93B0 glass and be a potential coating on bioactive glass for drug delivery purposes.

[1]  Yadong Zhang,et al.  Evaluation of an injectable bioactive borate glass cement to heal bone defects in a rabbit femoral condyle model. , 2017, Materials science & engineering. C, Materials for biological applications.

[2]  Yinan Lin,et al.  Bone Regeneration and Angiogenesis in Rat Calvarial Defects Implanted With Strong Porous Bioactive Glass (13‐93) Scaffolds Doped with Copper or Loaded with BMP2 , 2017 .

[3]  Dan Lin,et al.  Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect. , 2016, Acta biomaterialia.

[4]  L. Bonewald,et al.  Healing of critical-size segmental defects in rat femora using strong porous bioactive glass scaffolds. , 2014, Materials science & engineering. C, Materials for biological applications.

[5]  A. Boccaccini,et al.  Electrophoretic deposition of gentamicin-loaded bioactive glass/chitosan composite coatings for orthopaedic implants. , 2014, ACS applied materials & interfaces.

[6]  Robert A Latour,et al.  Quantification of the influence of protein-protein interactions on adsorbed protein structure and bioactivity. , 2013, Colloids and surfaces. B, Biointerfaces.

[7]  Jiabing Fan,et al.  Anionic carbohydrate-containing chitosan scaffolds for bone regeneration. , 2013, Carbohydrate polymers.

[8]  Chengtie Wu,et al.  Mesoporous bioactive glass scaffolds for efficient delivery of vascular endothelial growth factor , 2013, Journal of biomaterials applications.

[9]  L. Bonewald,et al.  Enhanced bone regeneration in rat calvarial defects implanted with surface-modified and BMP-loaded bioactive glass (13-93) scaffolds. , 2013, Acta biomaterialia.

[10]  常江,et al.  Mesoporous bioactive glass scaffolds for efficient delivery of vascular endothelial growth factor , 2013 .

[11]  Julian R Jones,et al.  Review of bioactive glass: from Hench to hybrids. , 2013, Acta biomaterialia.

[12]  B. Helgason,et al.  In vitro bioactivity of different degree of deacetylation chitosan, a potential coating material for titanium implants. , 2012, Journal of Biomedical Materials Research. Part A.

[13]  C. Canal,et al.  Calcium phosphate cements as drug delivery materials. , 2012, Advanced drug delivery reviews.

[14]  S. Şimon,et al.  FTIR and XPS studies of protein adsorption onto functionalized bioactive glass. , 2012, Biochimica et biophysica acta.

[15]  R. Vasita,et al.  Structural and functional characterization of proteins adsorbed on hydrophilized polylactide-co-glycolide microfibers , 2011, International journal of nanomedicine.

[16]  S. Totey,et al.  Chitosan enhances mineralization during osteoblast differentiation of human bone marrow‐derived mesenchymal stem cells, by upregulating the associated genes , 2011, Cell proliferation.

[17]  Eduardo Saiz,et al.  Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives. , 2011, Materials science & engineering. C, Materials for biological applications.

[18]  Delbert E Day,et al.  Bioactive glass in tissue engineering. , 2011, Acta biomaterialia.

[19]  M. Leu,et al.  Fabrication of 13-93 bioactive glass scaffolds for bone tissue engineering using indirect selective laser sintering , 2011, Biofabrication.

[20]  De-Hao Tsai,et al.  Adsorption and conformation of serum albumin protein on gold nanoparticles investigated using dimensional measurements and in situ spectroscopic methods. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[21]  A. Bandyopadhyay,et al.  Electrically polarized biphasic calcium phosphates: adsorption and release of bovine serum albumin. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[22]  Huaiyong Zhu,et al.  Bioactive mesopore-glass microspheres with controllable protein-delivery properties by biomimetic surface modification. , 2010, Journal of biomedical materials research. Part A.

[23]  Q. Fu,et al.  Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. I. Preparation and in vitro degradation. , 2010, Journal of biomedical materials research. Part A.

[24]  Keiichi Kuroki,et al.  Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation. , 2010, Journal of biomedical materials research. Part A.

[25]  Jennifer Patterson,et al.  Hyaluronic acid hydrogels with controlled degradation properties for oriented bone regeneration. , 2010, Biomaterials.

[26]  Laura A. Buchanan,et al.  Effect of bioactive glass crystallization on the conformation and bioactivity of adsorbed proteins. , 2009, Journal of biomedical materials research. Part A.

[27]  Changqing Zhang,et al.  Treatment of osteomyelitis and repair of bone defect by degradable bioactive borate glass releasing vancomycin. , 2009, Journal of controlled release : official journal of the Controlled Release Society.

[28]  R. Domingues,et al.  Tetracycline and/or hydrocortisone incorporation and release by bioactive glasses compounds , 2009 .

[29]  J. Bumgardner,et al.  Mechanical property, degradation rate, and bone cell growth of chitosan coated titanium influenced by degree of deacetylation of chitosan. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.

[30]  J. Kong,et al.  Fourier transform infrared spectroscopic analysis of protein secondary structures. , 2007, Acta biochimica et biophysica Sinica.

[31]  M. Rinaudo,et al.  Chitin and chitosan: Properties and applications , 2006 .

[32]  David J Mooney,et al.  Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. , 2006, Biomaterials.

[33]  P. Marquis,et al.  The influence of short and medium-term water immersion on the hydrolytic stability of novel low-shrink dental composites. , 2005, Dental materials : official publication of the Academy of Dental Materials.

[34]  Lorenz Meinel,et al.  Localized delivery of growth factors for bone repair. , 2004, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[35]  D. Achilias,et al.  Water sorption characteristics of light-cured dental resins and composites based on Bis-EMA/PCDMA. , 2004, Biomaterials.

[36]  N. Peppas,et al.  Structure and Interactions in Covalently and Ionically Crosslinked Chitosan Hydrogels for Biomedical Applications , 2003 .

[37]  J. Bumgardner,et al.  Chitosan: potential use as a bioactive coating for orthopaedic and craniofacial/dental implants , 2003, Journal of biomaterials science. Polymer edition.

[38]  M. Braden,et al.  Water absorption characteristics of dental composites incorporating hydroxyapatite filler. , 2002, Biomaterials.

[39]  S. Neau,et al.  In vitro degradation of chitosan by a commercial enzyme preparation: effect of molecular weight and degree of deacetylation. , 2001, Biomaterials.

[40]  A. Klibanov,et al.  FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres. , 1999, Journal of controlled release : official journal of the Controlled Release Society.

[41]  L. Hench,et al.  In vitro adsorption and activity of enzymes on reaction layers of bioactive glass substrates. , 1998, Journal of biomedical materials research.