Detection of dopamine in the pharmacy with a carbon nanotube paste electrode using voltammetry.

A simply prepared DNA immobilized on a carbon nanotube paste electrode (CNTPE) was utilized to monitor dopamine ion concentration using the cyclic voltammetry (CV) and square-wave (SW) stripping voltammetry methods. The optimum analytical conditions were sought. The result obtained was a very low detection limit compared to other common voltammetry methods. The optimal parameters were found to be as follows: 3.5 pH, 0.48 V SW amplitude, 71 Hz frequency, 5 s accumulation time, 0.01 V increment potential, and -1.3 V (anodic-*-) and 1.2 V (cathodic-o-) accumulation potentials. Given these conditions, the linear working range was observed to be within 0.01-0.11 microg L(-1) (SW anodic and CV). The analytical detection limit was determined to be SW anodic and CV: 4.0 microg L(-1) (2.1 x 10(-11) mol L(-1)) dopamin, and the relative standard deviation at the dopamine concentration of SW anodic 0.05 microg L(-1) was 0.02% (n=15) at the optimum conditions.

[1]  Akira Fujishima,et al.  Electroanalysis of dopamine and NADH at conductive diamond electrodes , 1999 .

[2]  Yuzhong Zhang,et al.  Study on the electrochemical behavior of dopamine with poly(sulfosalicylic acid) modified glassy carbon electrode , 2001 .

[3]  J Wang,et al.  Lab-on-a-Cable for electrochemical monitoring of phenolic contaminants. , 2000, Analytical chemistry.

[4]  Wlodzimierz Kutner,et al.  Electrocatalytic Properties and Sensor Applications of Fullerenes and Carbon Nanotubes , 2003 .

[5]  Shouzhuo Yao,et al.  Electrostatic assembly of calf thymus DNA on multi-walled carbon nanotube modified gold electrode and its interaction with chlorpromazine hydrochloride , 2004 .

[6]  Suw Young Ly,et al.  Electrochemical detection of ascorbic acid (vitamin C) using a glassy carbon electrode. , 2004, Die Nahrung.

[7]  M. Hows,et al.  High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples , 2004, Journal of Neuroscience Methods.

[8]  Itamar Willner,et al.  Biomolecule-functionalized carbon nanotubes: applications in nanobioelectronics. , 2004, Chemphyschem : a European journal of chemical physics and physical chemistry.

[9]  G. Torsi,et al.  Voltammetric trace metal determinations by cathodic and anodic stripping voltammetry in environmental matrices in the presence of mutual interference , 2001 .

[10]  Huimin Zhang,et al.  Electrocatalytic response of dopamine at a dl-homocysteine self-assembled gold electrode , 2000 .

[11]  G. Rivas,et al.  Adsorption and electrooxidation of nucleic acids at carbon nanotubes paste electrodes , 2004 .

[12]  Milton L. Lee,et al.  Determination of catecholamines and metanephrines in urine by capillary electrophoresis-electrospray ionization-time-of-flight mass spectrometry. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[13]  J. Pingarrón,et al.  Determination of micromolar bromate concentrations by adsorptive-catalytic stripping votammetry of the molybdenum-3-methoxy-4-hydroxymandelic acid complex. , 2001, Talanta.

[14]  S. Furlanetto,et al.  Simultaneous liquid chromatographic analysis of catecholamines and 4-hydroxy-3-methoxyphenylethylene glycol in human plasma. Comparison of amperometric and coulometric detection. , 2004, Journal of chromatography. A.

[15]  Zhi-Hong Liu,et al.  Microfabricated Disposable DNA Sensors Based on Enzymatic Amplification Electrochemical Detection , 2001 .

[16]  Myung-Hoon Kim,et al.  Square-wave cathodic stripping voltammetric analysis of RDX using mercury-film plated glassy carbon electrode. , 2002, Talanta.

[17]  Yuzuru Takamura,et al.  DNA-Directed Attachment of Carbon Nanotubes for Enhanced Label-Free Electrochemical Detection of DNA Hybridization , 2004 .

[18]  P. Kumarathasan,et al.  New approach to the simultaneous analysis of catecholamines and tyrosines in biological fluids. , 2003, Journal of chromatography. A.

[19]  Q. Wang,et al.  Electrocatalytic response of dopamine at a thiolactic acid self-assembled gold electrode , 2001 .

[20]  Alan M. Bond,et al.  Covalent modification of carbon electrodes for voltammetric differentiation of dopamine and ascorbic acid , 1995 .

[21]  Song Zhang,et al.  In vivo monitoring of the monoamine neurotransmitters in rat brain using microdialysis sampling with liquid chromatography electrochemical detection , 2001 .

[22]  E. Chan,et al.  High-performance liquid chromatographic assay for catecholamines and metanephrines using fluorimetric detection with pre-column 9-fluorenylmethyloxycarbonyl chloride derivatization. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[23]  G. Shi,et al.  Sensitive determination of dopamine on poly(aminobenzoic acid) modified electrode and the application toward an experimental Parkinsonian animal model. , 2001, Talanta.

[24]  G. Shen,et al.  Voltammetric behaviour of dopamine at nickel phthalocyanine polymer modified electrodes and analytical applications , 1997 .

[25]  Jing-Juan Xu,et al.  Interfacing cytochrome c to electrodes with a DNA: carbon nanotube composite film , 2002 .