New approach to phase and modulation resolved spectra

Author(s): Gratton, E; Jameson, DM | Abstract: Time domain fluorescence spectrometry offers a versatile and powerful approach to the analysis of heterogeneous emitting systems. In this paper we describe a new approach, based on software, to the acquisition of phase and modulation resolved spectra. Mixtures of fluorophores with different lifetimes can be analyzed in real time to give the individual excitation or emission spectra. Examples of two- and three-component mixtures are given and comparisons are made with the commercially available hardware approach. © 1985, American Chemical Society. All rights reserved.

[1]  D M Jameson,et al.  A simple digital integrator for real-time display of spectral areas. , 1977, Analytical biochemistry.

[2]  G. Weber,et al.  Resolution of the fluorescence lifetimes in a heterogeneous system by phase and modulation measurements , 1981 .

[3]  William R. Ware,et al.  Performance characteristics of a small side‐window photomultiplier in laser single‐photon fluorescence decay measurements , 1983 .

[4]  R. L. Blake,et al.  Technique for producing ultrathin polypropylene films , 1977 .

[5]  E. Selin,et al.  Background reduction of x-ray fluorescence spectra in a secondary target energy dispersive spectrometer , 1979 .

[6]  J. Lakowicz,et al.  DIRECT RECORDING OF THE INITIALLY EXCITED AND THE SOLVENT RELAXED FLUORESCENCE EMISSION SPECTRA OF TRYPTOPHAN BY PHASE SENSITIVE DETECTION OF FLUORESCENCE , 1982, Photochemistry and photobiology.

[7]  J. Lloyd.  Examination of petroleum products of high relative molecular mass for forensic purposes by synchronous fluorescence spectroscopy. Part I. Appraisal of experimental factors , 1980 .

[8]  H. Robberecht,et al.  Evaluation of multi-element analysis of blood serum by energy-dispersive x-ray spectrometry , 1982 .

[9]  E Gratton,et al.  Resolution of mixtures of fluorophores using variable-frequency phase and modulation data. , 1984, Biophysical journal.

[10]  K. Bisgard,et al.  Energy‐dispersive XRF spectrometry using secondary radiation in a cartesian geometry , 1981 .

[11]  J. Knutson,et al.  Decay-associated fluorescence spectra and the heterogeneous emission of alcohol dehydrogenase. , 1982, Biochemistry.

[12]  C. A. Parker,et al.  Spectrofluorimetry of lubricating oils: determination of oil mist in air , 1960 .

[13]  J. Lakowicz,et al.  Resolution of heterogeneous fluorescence from proteins and aromatic amino acids by phase-sensitive detection of fluorescence. , 1981, The Journal of biological chemistry.

[14]  Frank V. Bright,et al.  Phase-resolved fluorescence spectroscopy , 1984 .

[15]  E. Gratton,et al.  A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution. , 1983, Biophysical journal.

[16]  E. Wehry.  Modern Fluorescence Spectroscopy , 1981 .

[17]  L. McGown.  Phase-resolved fluorimetric determination of two albumin-bound fluorescein species , 1984 .

[18]  T. Vo‐Dinh,et al.  Direct determination of selected polynuclear aromatic hydrocarbons in a coal liquefaction product by synchronous luminescence techniques , 1981 .

[19]  G. WEBER,et al.  Enumeration of Components in Complex Systems by Fluorescence Spectrophotometry , 1961, Nature.

[20]  I. Soutar,et al.  Identification of crude oils by synchronous excitation spectrofluorimetry , 1976 .

[21]  J. Versieck,et al.  Sample contamination as a source of error in trace-element analysis of biological samples. , 1982, Talanta.

[22]  L. McGown,et al.  Comparison of phase-resolved and steady-state fluorometric multicomponent determinations using wavelength selection , 1984 .

[23]  P. Wobrauschek,et al.  X-ray fluorescence analysis with a linear polarized beam after bragg reflection from a flat or a curved single crystal , 1983 .

[24]  J. Lyons,et al.  Structural Interpretation of Fluorescence Spectra by Automated File Searching , 1981 .

[25]  T. Katoh,et al.  Analysis of a coal-derived liquid using highpressure liquid chromatography and synchronous fluorescence spectrometry , 1980 .

[26]  E Gratton,et al.  Multifrequency phase and modulation fluorometry. , 1984, Annual review of biophysics and bioengineering.

[27]  H. Schwenke,et al.  A new totally reflecting X-ray fluorescence spectrometer with detection limits below 10−11 g , 1980 .

[28]  J. Campbell,et al.  Specimen backings for proton-induced x-ray emission analysis , 1981 .

[29]  J. Beechem,et al.  Global resolution of heterogeneous decay by phase/modulation fluorometry: mixtures and proteins , 1983 .

[30]  E. Gratton,et al.  The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry , 1984 .

[31]  P. Espen,et al.  Automated energy-dispersive X-ray fluorescence analysis of environmental samples , 1977 .