Silica‐Gelatin Hybrids with Tailorable Degradation and Mechanical Properties for Tissue Regeneration

Nature has evolved mechanisms to create a diversity of specialized materials through nanoscale organization. Inspired by nature, hybrid materials are designed with highly tailorable properties, which are achieved through careful control of their nanoscale interactions. These novel materials, based on a silica-gelatin hybrid system, have the potential to serve as a platform technology for human tissue regeneration. Covalent interactions between the inorganic and organic constituents of the hybrid are essential to enable the precise control of mechanical and dissolution properties. Furthermore, hybrid scaffold porosity is found to highly influence mechanical properties, to the extent where scaffolds of particular strength could be specified based on their porosity. The hybrids also demonstrate a non-cytotoxic effect when mesenchymal stem cells are cultured on the material. Cytoskeletal proteins of the cells are imaged using actin and vimentin staining. It is envisaged these hybrid materials will find a diverse application in both hard and soft tissue regenerating scaffolds.

[1]  L L Hench,et al.  Direct chemical bond of bioactive glass-ceramic materials to bone and muscle. , 1973, Journal of biomedical materials research.

[2]  L. Ren,et al.  Synthesis and Characterization of Gelatin-Siloxane Hybrids Derived through Sol-Gel Procedure , 2001 .

[3]  F. Goubel,et al.  Macroscopic-microscopic characterization of the passive mechanical properties in rat soleus muscle. , 2006, Journal of biomechanics.

[4]  Shusheng Zhang,et al.  One-pot preparation of silica-supported hybrid immobilized metal affinity adsorbent with macroporous surface based on surface imprinting coating technique combined with polysaccharide incorporated sol--gel process. , 2006, Journal of chromatography. A.

[5]  P. Frenette,et al.  Signals from the Sympathetic Nervous System Regulate Hematopoietic Stem Cell Egress from Bone Marrow , 2006, Cell.

[6]  Juan L. Vivero-Escoto,et al.  Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers. , 2008, Advanced drug delivery reviews.

[7]  H J Helminen,et al.  Comparison of the equilibrium response of articular cartilage in unconfined compression, confined compression and indentation. , 2002, Journal of biomechanics.

[8]  I. Rehman,et al.  Synthesis and characterization of a novel fast-set proline-derivative-containing glass ionomer cement with enhanced mechanical properties. , 2009, Acta biomaterialia.

[9]  Suming Li,et al.  Hydrolytic degradation of devices based on poly(DL-lactic acid) size-dependence. , 1995, Biomaterials.

[10]  H. Ehrlich,et al.  Biomimetically inspired hybrid materials based on silicified collagen , 2007 .

[11]  R. Langer,et al.  Tissue engineering: current state and perspectives , 2004, Applied Microbiology and Biotechnology.

[12]  Evert Schepers,et al.  In vivo tissue response to resorbable silica xerogels as controlled-release materials. , 2005, Biomaterials.

[13]  H. Worch,et al.  A Novel Biomimetic Hybrid Material Made of Silicified Collagen: Perspectives for Bone Replacement , 2007 .

[14]  E. Place,et al.  Complexity in biomaterials for tissue engineering. , 2009, Nature materials.

[15]  G. Falini,et al.  Films of self-assembled purely helical type I collagen molecules , 2004 .

[16]  W. Hayes,et al.  Bone compressive strength: the influence of density and strain rate. , 1976, Science.

[17]  R N Vaishnav,et al.  Static Linear and Nonlinear Elastic Properties of Normal and Arterialized Venous Tissue in Dog and Man , 1975, Circulation research.

[18]  Donald E. Ingber,et al.  The riddle of morphogenesis: A question of solution chemistry or molecular cell engineering? , 1993, Cell.

[19]  Julian R Jones,et al.  Optimising bioactive glass scaffolds for bone tissue engineering. , 2006, Biomaterials.

[20]  Julian H. George,et al.  Exploring and Engineering the Cell Surface Interface , 2005, Science.

[21]  J. Inazawa,et al.  Damaged epithelia regenerated by bone marrow–derived cells in the human gastrointestinal tract , 2002, Nature Medicine.

[22]  J. Heino.  The collagen family members as cell adhesion proteins , 2007, BioEssays : news and reviews in molecular, cellular and developmental biology.

[23]  L. Hench,et al.  The sol-gel process , 1990 .

[24]  J. K. Leach,et al.  Proangiogenic Potential of a Collagen/Bioactive Glass Substrate , 2008, Pharmaceutical Research.

[25]  Kanji Tsuru,et al.  Novel approach to fabricate porous gelatin-siloxane hybrids for bone tissue engineering. , 2002, Biomaterials.

[26]  M. Pittenger,et al.  Multilineage potential of adult human mesenchymal stem cells. , 1999, Science.

[27]  S F Hulbert,et al.  Tissue reaction to three ceramics of porous and non-porous structures. , 1972, Journal of biomedical materials research.

[28]  Fusheng Pan,et al.  PVA–GPTMS/TEOS hybrid pervaporation membrane for dehydration of ethylene glycol aqueous solution , 2006 .

[29]  B. Riegel,et al.  FT-Raman Spectroscopic Investigations on the Organic Crosslinking in Hybrid Polymers Part II: Reactions of Epoxy Silanes , 2002 .

[30]  L L Hench,et al.  Surface-active biomaterials. , 1984, Science.

[31]  Bruce M. Novak,et al.  Hybrid nanocomposite materials―between inorganic glasses and organic polymers , 1993 .

[32]  T Kitsugi,et al.  Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. , 1990, Journal of biomedical materials research.

[33]  K. Warrier,et al.  Synthesis of biocompatible hydrophobic silica-gelatin nano-hybrid by sol-gel process. , 2007, Colloids and surfaces. B, Biointerfaces.

[34]  L. Griffith,et al.  Tissue Engineering--Current Challenges and Expanding Opportunities , 2002, Science.

[35]  Larry L Hench,et al.  Third-Generation Biomedical Materials , 2002, Science.

[36]  Min Wang,et al.  Developing bioactive composite materials for tissue replacement. , 2003, Biomaterials.

[37]  T. Coradin,et al.  Gelatine/silicate interactions: from nanoparticles to composite gels. , 2004, Colloids and surfaces. B, Biointerfaces.

[38]  T. Buckland,et al.  Comparative performance of three ceramic bone graft substitutes. , 2007, The spine journal : official journal of the North American Spine Society.

[39]  Julian R. Jones,et al.  Effect of surfactant concentration and composition on the structure and properties of sol-gel-derived bioactive glass foam scaffolds for tissue engineering , 2003 .

[40]  K. Warrier,et al.  Silica–gelatin bio-hybrid and transparent nano-coatings through sol–gel technique , 2007 .

[41]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.

[42]  M. Ashby,et al.  Cellular solids: Structure & properties , 1988 .

[43]  Jun Wang,et al.  Preparation and luminescence properties of ormosil hybrid materials doped with Tb(Tfacac)3phen complex via a sol–gel process , 2003 .

[44]  G. Kickelbick,et al.  Concepts for the incorporation of inorganic building blocks into organic polymers on a nanoscale , 2003 .

[45]  K. V. Van Vliet,et al.  Probing mechanical properties of fully hydrated gels and biological tissues. , 2008, Journal of biomechanics.

[46]  Aldo R Boccaccini,et al.  45S5 Bioglass-derived glass-ceramic scaffolds for bone tissue engineering. , 2006, Biomaterials.

[47]  J. Hubbell,et al.  Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering , 2005, Nature Biotechnology.

[48]  Julian R Jones,et al.  Bioactive sol-gel foams for tissue repair. , 2002, Journal of biomedical materials research.

[49]  Qizhi Chen,et al.  Biomaterials in cardiac tissue engineering: Ten years of research survey , 2008 .

[50]  Julian R. Jones,et al.  Nanostructure evolution and calcium distribution in sol-gel derived bioactive glass , 2009 .