Scanning electron microscopy of the leaf epidermis in Arundinarieae (Poaceae: Bambusoideae): evolutionary implications of selected micromorphological features

Arundinarieae (temperate woody bamboos) is a taxonomically challenging group and the taxa in this tribe show a low level of DNA sequence variation, especially for plastid markers. As a result of the scarcity of flowering collections, species identification mainly depends on vegetative features, and leaf epidermal micromorphology has proven to be useful in bamboo taxonomy. In this study, we used scanning electron microscopy to investigate the abaxial leaf epidermal micromorphology of 94 species in 24 genera of Arundinarieae and three species of Bambuseae. Leaf epidermal characteristics differed mainly in the number and distribution pattern of papillae. Seven papilla forms were assigned, which had little taxonomic value at the generic and subtribal levels. However, the papilla patterns combined with other leaf epidermal features were useful in the discrimination of several species. Most papilla types were shared between tribes Arundinarieae and Bambuseae. We assessed the phylogenetic implications of the leaf epidermal micromorphology based on recently published molecular phylogenetic analyses. With one exception, none of the papilla types corresponded to a particular clade in the plastid or nuclear phylogenetic trees. Based on these results, the papilla patterns have limited phylogenetic value. (C) 2014 The Linnean Society of London

[1]  Jun-bo Yang,et al.  The monophyly of Chimonocalamus and conflicting gene trees in Arundinarieae (Poaceae: Bambusoideae) inferred from four plastid and two nuclear markers. , 2013, Molecular phylogenetics and evolution.

[2]  P. Viana,et al.  A New Woody Bamboo (Poaceae, Bambusoideae) from Central Brazil, Aulonemia xerophylla , 2013 .

[3]  S. Kelchner,et al.  Higher level phylogenetic relationships within the bamboos (Poaceae: Bambusoideae) based on five plastid markers. , 2013, Molecular phylogenetics and evolution.

[4]  R. P. Oliveira,et al.  Eremitis afimbriata and E. magnifica (Poaceae, Bambusoideae, Olyreae): two remarkable new species from Brazil and a first record of blue iridescence in bamboo leaves , 2013 .

[5]  L. Clark,et al.  Cambajuva (Poaceae: Bambusoideae: Bambuseae: Arthrostylidiinae), a New Woody Bamboo Genus from Southern Brazil , 2013 .

[6]  Lianming Gao,et al.  Testing four candidate barcoding markers in temperate woody bamboos (Poaceae: Bambusoideae) , 2012 .

[7]  Chun-Xia Zeng,et al.  Complex evolution in Arundinarieae (Poaceae: Bambusoideae): incongruence between plastid and nuclear GBSSI gene phylogenies. , 2012, Molecular phylogenetics and evolution.

[8]  L. Junior,et al.  Comparative analysis of the leaf anatomy in two Parodiolyra species (Poaceae: Olyreae) occurring on forests in Eastern Brazil. , 2012, Brazilian journal of biology = Revista brasleira de biologia.

[9]  M. A. Lizarazu,et al.  A New Species of Guadua (Poaceae, Bambusoideae, Bambuseae) and Synopsis of the Genus in Argentina and Neighboring Regions , 2011 .

[10]  De‐Zhu Li,et al.  Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants , 2011, Proceedings of the National Academy of Sciences.

[11]  P. Viana,et al.  A new combination in Aulonemia (Poaceae: Bambusoideae: Bambuseae) based on floral analysis, anatomical features, and distribution , 2011, Brittonia.

[12]  L. Clark,et al.  Phylogeny of the Temperate Bamboos (Poaceae: Bambusoideae: Bambuseae) with an Emphasis on Arundinaria and Allies , 2010 .

[13]  M. Chase,et al.  Phylogenetic analyses of plastid and nuclear DNA sequences indicate a rapid late Miocene radiation of the temperate bamboo tribe Arundinarieae (Poaceae, Bambusoideae) , 2010 .

[14]  De‐Zhu Li,et al.  Large multi-locus plastid phylogeny of the tribe Arundinarieae (Poaceae: Bambusoideae) reveals ten major lineages and low rate of molecular divergence. , 2010, Molecular phylogenetics and evolution.

[15]  Ya-yin Chen,et al.  Microarray analysis reveals the inhibition of nuclear factor-kappa B signaling by aristolochic acid in normal human kidney (HK-2) cells , 2010, Acta Pharmacologica Sinica.

[16]  L. J. Neves,et al.  Scanning electron microscopy of the leaf epidermis of Merostachys Spreng. (Poaceae: Bambusoideae) , 2009 .

[17]  De-Zhu Li,et al.  Highly heterogeneous generic delimitation within the temperate bamboo clade (Poaceae: Bambusoideae) : evidence from GBSSI and ITS sequences , 2008 .

[18]  M. Chase,et al.  Large multi-gene phylogenetic trees of the grasses (Poaceae): progress towards complete tribal and generic level sampling. , 2008, Molecular phylogenetics and evolution.

[19]  Reyjane Patrícia de Oliveira,et al.  A contribuição da anatomia foliar para a taxonomia de Raddia Bertol. (Poaceae: Bambusoideae) , 2008 .

[20]  Hong Wang,et al.  Comparative morphology of the foliage leaf epidermis, with emphasis on papillae characters, in key taxa of woody bamboos of the Asian tropics (Poaceae: Bambusoideae) , 2008 .

[21]  D. Janzen,et al.  Use of DNA barcodes to identify flowering plants. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Jeremy R. deWaard,et al.  Biological identifications through DNA barcodes , 2003, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[23]  R. Vieira,et al.  Leaf anatomy of three herbaceous bamboo species. , 2002, Brazilian journal of biology = Revista brasleira de biologia.

[24]  Chen Xiao-ya,et al.  Micromorphology of Leaf Epidermis of Chimonobambusa (Bambusoideae) , 1993 .

[25]  L. Clark,et al.  A new species and new sections of Rhipidocladum (Poaceae: Bambusoideae) , 1991 .

[26]  R. Ellis.  A procedure for standardizing comparative leaf anatomy in the Poaceae. II. The epidermis as seen in surface view , 1979 .

[27]  E. Judziewicz,et al.  THREE NEW ANDEAN SPECIES OF AULONEMIA (POACEAE: BAMBUSOIDEAE: BAMBUSEAE) WITH SHEATH AURICLES , 2011 .

[28]  Nicolas Salamin,et al.  Non-monophyly of the woody bamboos (Bambuseae; Poaceae): a multi-gene region phylogenetic analysis of Bambusoideae s.s. , 2008, Journal of Plant Research.

[29]  D. Ohrnberger.  The bamboos of the world: annotated nomenclature and literature of the species and the higher and lower taxa , 1999 .

[30]  Dz Z. Li.  The Flora of China Bambusoideae Project - problems and current understanding of bamboo taxonomy in China , 1997 .

[31]  B. Gardiner,et al.  Linnean Society of London , 1956, Nature.