Degradation of pig gastric and colonic mucins by bacteria isolated from the pig colon

Mucin degradation was studied with one Clostridium (RS42) and two Bacteroides (RS2 and RS13) strains isolated from the pig colon mucosa. Mucins from pig colon and stomach were prepared in their subunit forms for use as growth substrates, and the loss of the individual sugars from the mucins was measured after bacterial growth. Colonic mucin was more resistant to degradation than gastric mucin. The strains differed in their competence in degrading the mucins. Carbohydrate plus sulfate removal from gastric mucin varied from 63 to 76% for RS2, 37 to 46% for RS13, and 37 to 53% for RS42. All three strains removed more fucose (67 to 87%) and less sulfate (22 to 63%) than the average carbohydrate plus sulfate loss. Under the same conditions of growth, a mixed pig fecal culture removed 78% of sulfate and 96% of each sugar. Of the two major glycoprotein types present in the subunit pig gastric mucin preparation (R. A. Stanley, S. P. Lee, and A. M. Roberton, Biochim. Biophys. Acta 760:262-269, 1983), the less highly sulfated mucin was more susceptible to RS42 degradation. The degradation of gastric mucin by RS2 was not affected by glucose or high sulfate concentrations in the growth medium. The results show that the three strains of colon bacteria are capable of significant hydrolysis of mucin carbohydrate and that the extent of degradation seen with pure cultures is determined in part by the subunit glycoprotein type(s) present in the mucin.

[1]  L. Hoskins,et al.  Mucin degradation in human colon ecosystems. Isolation and properties of fecal strains that degrade ABH blood group antigens and oligosaccharides from mucin glycoproteins. , 1985, The Journal of clinical investigation.

[2]  A. Slomiany,et al.  The role of covalently bound fatty acids in the degradation of human gastric mucus glycoprotein. , 1984, Archives of biochemistry and biophysics.

[3]  A. M. Roberton,et al.  Heterogeneity in gastrointestinal mucins. , 1983, Biochimica et biophysica acta.

[4]  A. Slomiany,et al.  Lipids associated with rat small-intestinal mucus glycoprotein. , 1983, Carbohydrate research.

[5]  R. Hurst,et al.  Analysis of sulfate in complex carbohydrates. , 1982, Analytical biochemistry.

[6]  C. Edmiston,et al.  Anaerobic bacterial populations on normal and diseased human biopsy tissue obtained at colonoscopy , 1982, Applied and environmental microbiology.

[7]  A. M. Roberton,et al.  In vitro utilization of mucin by Bacteroides fragilis , 1982, Applied and environmental microbiology.

[8]  S. Barondes,et al.  Secretion of endogenous lectin by chicken intestinal goblet cells , 1982, The Journal of cell biology.

[9]  R. Miller,et al.  Mucin degradation in human colon ecosystems. Fecal population densities of mucin-degrading bacteria estimated by a "most probable number" method. , 1981, Gastroenterology.

[10]  R J Miller,et al.  A colorimetric assay for mucous glycoproteins using Alcian Blue [proceedings]. , 1980, Biochemical Society transactions.

[11]  E. G. Russell.  Types and Distribution of Anaerobic Bacteria in the Large Intestine of Pigs , 1979, Applied and environmental microbiology.

[12]  N. Tolbert,et al.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. , 1978, Analytical biochemistry.

[13]  A. Allen,et al.  A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain [proceedings]. , 1978, Biochemical Society transactions.

[14]  J. Vercellotti,et al.  Breakdown of mucin and plant polysaccharides in the human colon. , 1977, Canadian journal of biochemistry.

[15]  S. E. West,et al.  Fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon , 1977, Applied and environmental microbiology.

[16]  K. Winkler,et al.  The faecal flora in ulcerative colitis. , 1975, Journal of medical microbiology.

[17]  W. C. Breckenridge,et al.  Analysis of monosaccharides by gas-liquid chromatography of the O-methyl glycosides as trifluoroacetate derivatives. Application to glycoproteins and glycolipids. , 1972, Journal of chromatography.

[18]  N. Zamcheck,et al.  Bacterial degradation of gastrointestinal mucins. I. Comparison of mucus constituents in the stools of germ-free and conventional rats. , 1968, Gastroenterology.

[19]  S. Lindstedt,et al.  MUCUS IN INTESTINAL CONTENTS OF GERMFREE RATS , 1965, The Journal of experimental medicine.

[20]  L. Warren,et al.  The thiobarbituric acid assay of sialic acids. , 1959, The Journal of biological chemistry.

[21]  R. Bailey.  The reaction of pentoses with anthrone. , 1958, The Biochemical journal.

[22]  W. Cunliffe,et al.  Studies on gastrointestinal mucus. , 1984, Scandinavian journal of gastroenterology. Supplement.

[23]  L C Hoskins,et al.  Mucin degradation in human colon ecosystems. Evidence for the existence and role of bacterial subpopulations producing glycosidases as extracellular enzymes. , 1981, The Journal of clinical investigation.

[24]  Lillian V. Holdeman,et al.  Anaerobe Laboratory manual , 1977 .