Stumbling upon a New Frog Species of Guibemantis (Anura: Mantellidae) on Top of the Marojejy Massif in Northern Madagascar

We describe a new frog species of the genus Guibemantis, subgenus Pandanusicola, from Marojejy National Park in northeastern Madagascar. Guibemantis milingilingy, the new species, exhibits high genetic divergence from all other described species of Guibemantis based on DNA sequences of a fragment of the mitochondrial 16S rRNA gene. This new species inhabits Pandanus plants like the majority of other members of this subgenus, but is found at much higher elevations (2026 m a.s.l.). It differs from its closest relative, G. punctatus, by the lack of speckled or spotted dorsal patterns and by the presence of a distinctly dark tympanum. As a high-elevation species, it is likely to be a species sensitive to climate change, similar to other montane endemics in Madagascar.

[1]  M. Vences,et al.  Yet another small brown frog from high altitude on the Marojejy Massif, northeastern Madagascar (Anura: Mantellidae). , 2017, Zootaxa.

[2]  M. Vences,et al.  The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. , 2017, Zootaxa.

[3]  R. Geffers,et al.  Freshwater vertebrate metabarcoding on Illumina platforms using double-indexed primers of the mitochondrial 16S rRNA gene , 2016, Conservation Genetics Resources.

[4]  Sudhir Kumar,et al.  MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. , 2016, Molecular biology and evolution.

[5]  C. Raxworthy,et al.  A new phytotelmic species of Platypelis (Microhylidae: Cophylinae) from the Betampona Reserve, eastern Madagascar , 2014 .

[6]  M. Vences,et al.  A new microhylid frog, genus Rhombophryne, from northeastern Madagascar, and a re-description of R. serratopalpebrosa using micro-computed tomography. , 2014, Zootaxa.

[7]  M. Vences,et al.  DNA barcoding Madagascar's amphibian fauna , 2014 .

[8]  M. Vences,et al.  Assessing the diversity of phytotelmic frogs along Madagascar’s east coast: Redefinition of Guibemantis bicalcaratus (BOETTGER, 1913) and revalidation of Guibemantis methueni (ANGEL, 1929) , 2013, Vertebrate Zoology.

[9]  Ramón Doallo,et al.  CircadiOmics: integrating circadian genomics, transcriptomics, proteomics and metabolomics , 2012, Nature Methods.

[10]  M. Vences,et al.  A tiny new species of Platypelis from the Marojejy National Park in northeastern Madagascar (Amphibia: Microhylidae) , 2012 .

[11]  Maxim Teslenko,et al.  MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space , 2012, Systematic biology.

[12]  M. Vences,et al.  Speciation in little: the role of range and body size in the diversification of Malagasy mantellid frogs , 2011, BMC Evolutionary Biology.

[13]  M. Vences,et al.  New Species of Gephyromantis from Marojejy National Park, Northeast Madagascar , 2011 .

[14]  M. Vences,et al.  Two New Plant-Breeding Frog Species (Anura: Mantellidae, Guibemantis) from Southeastern Madagascar , 2011 .

[15]  M. Vences,et al.  Vast underestimation of Madagascar's biodiversity evidenced by an integrative amphibian inventory , 2009, Proceedings of the National Academy of Sciences.

[16]  D. Stone,et al.  Extinction vulnerability of tropical montane endemism from warming and upslope displacement: a preliminary appraisal for the highest massif in Madagascar , 2008, Global Change Biology.

[17]  M. Vences,et al.  Mitochondrial genes reveal cryptic diversity in plant-breeding frogs from Madagascar (Anura, Mantellidae, Guibemantis). , 2007, Molecular phylogenetics and evolution.

[18]  C. Raxworthy,et al.  Six New Species of Occipital-lobed Calumma Chameleons (Squamata: Chamaeleonidae) from Montane Regions of Madagascar, with a New Description and Revision of Calumma Brevicorne , 2006, Copeia.

[19]  Richard M. Lehtinen TESTS FOR COMPETITION, CANNIBALISM, AND PRIORITY EFFECTS IN TWO PHYTOTELM-DWELLING TADPOLES FROM MADAGASCAR1 , 2004 .

[20]  F. Andreone Crossroads of herpetological diversity: Survey work for an integrated conservation of amphibians and reptiles in northern Madagascar , 2004 .

[21]  Richard M. Lehtinen Parental Care and Reproduction in Two Species of Mantidactylus (Anura: Mantellidae) , 2003 .

[22]  Richard M. Lehtinen The use of screw pines (Pandanus spp.) by amphibians and reptiles in Madagascar , 2002 .

[23]  M. Vences,et al.  Systematic revision of the enigmatic Malagasy broad-headed frogs (Laurentomantis Dubois, 1980), and their phylogenetic position within the endemic mantellid radiation of Madagascar , 2002 .

[24]  M. Vences,et al.  Revision of the Boophis majori group (Amphibia: Mantellidae) from Madagascar, with descriptions of five new species , 2001 .

[25]  D. Vallan Influence of forest fragmentation on amphibian diversity in the nature reserve of Ambohitantely, highland Madagascar , 2000 .

[26]  M. Vences,et al.  A new species of Mantidactylus (subgenus Guibemantis) from Madagascar, with a comparative survey of internal femoral gland structure in the genus (Amphibia: Ranidae: Mantellinae) , 2000 .

[27]  M. Vences,et al.  A review of the genus Mantella (Anura, Ranidae, Mantellinae): taxonomy, distribution and conservation of malagasy poison frogs , 1999 .

[28]  J. Brookfield,et al.  Single-locus and multilocus DNA fingerprinting. , 1992 .

[29]  R. Blommers-Schlösser Biosystematics of the Malagasy frogs. I. Mantellinae (Ranidae) , 1979 .