Evaluation of Antioxidant Potential of Hedychium spicatum Rhizome Extracts from Bhowali Region, Uttarakhand, India

Background: It is well-known that different extraction methods, including the technique, solvents, time and temperature extensively influence the antioxidant activity of plant secondary metabolites. In our study, Hedychium spicatum was used as a plant sample, collected from Bhowali region, Uttarakhand, India, using two extracting solvents (acetone and chloroform) to explore the antioxidant activity, total phenolic and flavonoid contents (TPC and TFC). Methods: Initial phytochemical analysis was performed by evaluating the TPC and TFC content by Folin-Ciocalteu and AlCl3 colorimetric assay. For the estimation of antioxidant activity of test samples, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods were used for the determination of free radical scavenging activity, respectively. Results: The FRAP results of acetone and chloroform samples was 335.782 and 254.116 μM FeSO4·7H2O/g of dry extract, respectively. IC50 values for acetone and chloroform extract were calculated and used to interpret DPPH radical scavenging activity. Both tested extracts exhibited potent DPPH radical scavenging activity having IC50, 113.11 and 294.23 μg/ml for acetone and chloroform extract, respectively. The result of TPC (12.82 mg equivalent to gallic acid) and TFC (13.998 mg equivalent to quercetin/gram) of dry extract respectively. Conclusions: The overall results exhibit the high antioxidant potentiality of acetone extract as compared to chloroform extract, which could be due to its high phenolic and flavonoid content presence. Key-words: Antioxidant potential, free radical scavenging activity, Hedychium spicatum, Medicinal plants, Plant extract, IC50

[1]  Ying Zhou,et al.  Phytochemical Analysis, Antioxidant, Antibacterial, Cytotoxic, and Enzyme Inhibitory Activities of Hedychium flavum Rhizome , 2020, Frontiers in Pharmacology.

[2]  Ming-Hua Yang,et al.  Estimation of total flavonoid content in propolis by two complementary colometric methods , 2020, Journal of Food and Drug Analysis.

[3]  A. Seca,et al.  Uncharted Source of Medicinal Products: The Case of the Hedychium Genus , 2020, Medicines.

[4]  V. Alves,et al.  Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications , 2019, Molecules.

[5]  T. Isah.  Stress and defense responses in plant secondary metabolites production , 2019, Biological Research.

[6]  J. Mattei,et al.  The Role of Polyphenols in Human Health and Food Systems: A Mini-Review , 2018, Front. Nutr..

[7]  Duangjai Tungmunnithum,et al.  Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview , 2018, Medicines.

[8]  A. Jugran,et al.  Hedychium spicatum: a systematic review on traditional uses, phytochemistry, pharmacology and future prospectus , 2018, The Journal of pharmacy and pharmacology.

[9]  Yingxian Zhao,et al.  Response of Plant Secondary Metabolites to Environmental Factors , 2018, Molecules.

[10]  V. Gupta,et al.  Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India , 2016, Front. Plant Sci..

[11]  Fereidoon Shahidi,et al.  Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review , 2015 .

[12]  J. Jacquier,et al.  Stability of phytochemicals as sources of anti-inflammatory nutraceuticals in beverages — A review , 2013 .

[13]  P. M. Paarakh,et al.  Antioxidant activity of Hedychium spicatum Buch.- Ham. rhizomes , 2012 .

[14]  P. Casati,et al.  Flavonoids: biosynthesis, biological functions, and biotechnological applications , 2012, Front. Plant Sci..

[15]  R. Rawal,et al.  Total phenolic compounds and antioxidant potential of Hedychium spicatum Buch. Ham. ex D. Don in west Himalaya, India , 2011 .

[16]  A. S. Babji,et al.  Antioxidant activity of plants methanolic extracts containing phenolic compounds , 2009 .

[17]  J. Markowski,et al.  Simple method for determining human serum 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity – possible application in clinical studies on dietary antioxidants , 2008, Clinical chemistry and laboratory medicine.

[18]  O. Prakash,et al.  Terpenoid Compositions, and Antioxidant and Antimicrobial Properties of the Rhizome Essential Oils of Different Hedychium Species , 2008, Chemistry & biodiversity.

[19]  J. Pincemail,et al.  Evolution of antioxidant capacity during storage of selected fruits and vegetables. , 2007, Journal of agricultural and food chemistry.

[20]  T. Dhole,et al.  Antimicrobial activity of Hedychium spicatum. , 2006, Fitoterapia.

[21]  Mei Sun,et al.  Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer , 2004, Life Sciences.

[22]  J. Koricheva,et al.  Seasonal changes in birch leaf chemistry: are there trade-offs between leaf growth and accumulation of phenolics? , 2002, Oecologia.

[23]  C. Rice-Evans,et al.  Antioxidant properties of phenolic compounds , 1997 .

[24]  Manoj Kumar Singh,et al.  Plants as a Source of Potential Antioxidants and Their Effective Nanoformulations , 2021, Journal of Scientific Research.

[25]  Manoj Kumar Singh,et al.  Scrutinizing the antioxidant potential of Prunella vulgaris L. : A medicinal plant from central Himalayan region , 2015 .

[26]  R. Rawal,et al.  PHCOG MAG.:Research Article Evaluation of antioxidant phytochemical diversity in Hedychium spicatum : a high value medicinal plant of Himalaya , 2008 .