Interaction of dehydroepiandrosterone with phospholipid membranes: an infrared spectroscopy investigation.

The interaction between dehydroepiandrosterone (DHEA) and its sulfate metabolite (DHEA-S) with deuterated dimirystoylphosphatidylcholine (DMPC-d54) was investigated by FTIR spectroscopy. DHEA, as cholesterol, induces some conformational order in the liquid-crystalline phase of DMPC-d54. Attenuated total reflectance (ATR) measurements performed on oriented DMPC-d54/steroids samples have shown that in the gel phase, the acyl chains of DMPC-d54 become more normal to the bilayer surface in the presence of DHEA or cholesterol. On the other hand, DHEA-S increases the number of gauche conformers along the hydrocarbon chains of DMPC-d54. No evidence for the presence of hydrogen bond was found between both steroids and the 13C labeled carbonyl group of hydrated DMPC.

[1]  C. Vilchèze,et al.  Interactions of cholesterol and synthetic sterols with phosphatidylcholines as deduced from infrared CH2 wagging progression intensities , 1993 .

[2]  A. Sygula,et al.  Investigations of dehydroepiandrosterone. Part I: crystal structure of sublimed DHEA , 1996 .

[3]  J. Boggs,et al.  Study by infrared spectroscopy of the interaction of bovine myelin basic protein with phosphatidic acid. , 1994, Biochemistry.

[4]  H. Mantsch,et al.  The thermotropic phase behavior of N-methylated dipalmitoylphosphatidylethanolamines , 1983 .

[5]  M. Lafleur,et al.  Contribution of the Intermolecular Coupling and Librotorsional Mobility in the Methylene Stretching Modes in the Infrared Spectra of Acyl Chains , 1994 .

[6]  Robert B. Gennis,et al.  Biomembranes: Molecular Structure and Function , 1988 .

[7]  H. Mantsch,et al.  Components of the carbonyl stretching band in the infrared spectra of hydrated 1,2-diacylglycerolipid bilayers: a reevaluation. , 1994, Biophysical journal.

[8]  H. Mantsch,et al.  A Fourier transform infrared spectroscopic study of the molecular interaction of cholesterol with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. , 1980, Biochimica et biophysica acta.

[9]  J. Goméz,et al.  Changes in serum concentrations of conjugated and unconjugated steroids in 40- to 80-year-old men. , 1994, The Journal of clinical endocrinology and metabolism.

[10]  H. Mantsch,et al.  Hydrogen bonding between anhydrous cholesterol and phosphatidylcholines: an infrared spectroscopic study. , 1989, Biochimica et biophysica acta.

[11]  R. McElhaney,et al.  New aspects of the interaction of cholesterol with dipalmitoylphosphatidylcholine bilayers as revealed by high-sensitivity differential scanning calorimetry. , 1995, Biochimica et biophysica acta.

[12]  Douglas J. Moffatt,et al.  Precision in Condensed Phase Vibrational Spectroscopy , 1982 .

[13]  R. Mendelsohn,et al.  Methylene wagging progressions as IR probes of slightly disordered phospholipid acyl chain states , 1992 .

[14]  E. Dufourc,et al.  Structural and dynamical details of cholesterol-lipid interaction as revealed by deuterium NMR , 1984 .

[15]  A. Blume,et al.  Fourier transform infrared spectroscopy of 13C = O-labeled phospholipids hydrogen bonding to carbonyl groups. , 1988, Biochemistry.

[16]  C. Lafrance,et al.  On the relationship between the order parameter and the shape of orientation distributions , 1995 .

[17]  P. Yeagle.  Cholesterol and the cell membrane. , 1985, Biochimica et biophysica acta.

[18]  H. Mantsch,et al.  Phospholipid phase transitions in model and biological membranes as studied by infrared spectroscopy. , 1991, Chemistry and physics of lipids.

[19]  J. Boggs,et al.  Study by infrared spectroscopy of the interdigitation of C26:0 cerebroside sulfate into phosphatidylcholine bilayers. , 1996, Biochemistry.

[20]  P. Griffiths,et al.  Introduction to spectral deconvolution , 1986 .

[21]  P. Cullis,et al.  The influence of cholesterol 3-sulphate on phase behaviour and hydrocarbon order in model membrane systems. , 1992, Biochimica et biophysica acta.

[22]  R. Lewis,et al.  Comparative differential scanning calorimetric and FTIR and 31P-NMR spectroscopic studies of the effects of cholesterol and androstenol on the thermotropic phase behavior and organization of phosphatidylcholine bilayers. , 1994, Biophysical journal.

[23]  H. Mantsch,et al.  Polymorphic phase behaviour of phospholipid membranes studied by infrared spectroscopy. , 1984, Biochimica et biophysica acta.

[24]  T. Tsong,et al.  The influence of cholesterol on the main phase transition of unilamellar dipalmytoylphosphatidylcholine vesicles. A differential scanning calorimetry and iodine laser T-jump study. , 1986, Biophysical journal.

[25]  M. Schumacher,et al.  Development and Regeneration of the Nervous System: A Role for Neurosteroids (Paart 1 of 2) , 1996 .

[26]  E. Dufourc,et al.  Comparative effects of cholesterol and cholesterol sulfate on hydration and ordering of dimyristoylphosphatidylcholine membranes. , 1996, Biophysical journal.

[27]  M. Bloom,et al.  Combined influence of cholesterol and synthetic amphiphillic peptides upon bilayer thickness in model membranes. , 1992, Biophysical journal.