Response to Comments on “A Bacterium That Can Grow Using Arsenic Instead of Phosphorus”

Concerns have been raised about our recent study suggesting that arsenic (As) substitutes for phosphorus in major biomolecules of a bacterium that tolerates extreme As concentrations. We welcome the opportunity to better explain our methods and results and to consider alternative interpretations. We maintain that our interpretation of As substitution, based on multiple congruent lines of evidence, is viable.

[1]  B. Rosen,et al.  Arsenate reductases in prokaryotes and eukaryotes. , 2002, Environmental health perspectives.

[2]  Kevin A Francesconi,et al.  Determination of arsenic species: a critical review of methods and applications, 2000-2003. , 2004, The Analyst.

[3]  T. N. Bhat,et al.  The Protein Data Bank , 2000, Nucleic Acids Res..

[4]  E. Kay.  Incorporation of Radioarsenate into Proteins and Nucleic Acids of the Ehrlich Lettré Ascites Carcinoma In Vitro , 1965, Nature.

[5]  R. Summons,et al.  SYNTHESIS OF -TRIMETHYLARSONIUMLACTATE , 1982 .

[6]  A. Anbar,et al.  A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus , 2011, Science.

[7]  J. Scott,et al.  Freshwater Bacteria are Stoichiometrically Flexible with a Nutrient Composition Similar to Seston , 2010, Front. Microbio..

[8]  J. Cotner,et al.  Comment on “A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus” , 2011, Science.

[9]  R. Oremland,et al.  Bacterial respiration of arsenic and selenium. , 1999, FEMS microbiology reviews.

[10]  R. D. Hamilton,et al.  ADENOSINE TRIPHOSPHATE CONTENT OF MARINE BACTERIA1 , 1967 .

[11]  A. Ponce,et al.  Comment on “A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus” , 2011, Science.

[12]  Eörs Szathmáry,et al.  Comment on “A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus” , 2011, Science.

[13]  J. Briand,et al.  XAS study of arsenic coordination in Euglena gracilis exposed to arsenite. , 2008, Environmental science & technology.

[14]  A. Anbar,et al.  Did nature also choose arsenic? , 2009, International Journal of Astrobiology.

[15]  R. Redfield.  Comment on “A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus” , 2011, Science.

[16]  B. Rosen,et al.  Biochemistry of arsenic detoxification , 2002, FEBS letters.

[17]  M. Klotz.  The Grand Challenge of Microbiology: To Know Better, Protect, Utilize and Celebrate the Unseen Majority on Our Planet , 2010, Front. Microbio..

[18]  R. Oremland,et al.  Coupled Arsenotrophy in a Hot Spring Photosynthetic Biofilm at Mono Lake, California , 2010, Applied and Environmental Microbiology.

[19]  J. Elser,et al.  Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere , 2002 .

[20]  A. Benson,et al.  Arsoniumphospholipid in algae. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[21]  Robert W. Sterner,et al.  Are bacteria more like plants or animals? Growth rate and resource dependence of bacterial C : N : P stoichiometry , 2003 .

[22]  P. Foster.  Comment on “A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus” , 2011, Science.