Heterologous and cell free protein expression systems.
暂无分享,去创建一个
[1] Luke A Miles. Robust and Cost‐effective Cell‐free Expression of Biopharmaceuticals: Escherichia Coli and Wheat Embryo , 2008 .
[2] A. Engel,et al. Cell-free production of G protein-coupled receptors for functional and structural studies. , 2007, Journal of structural biology.
[3] S. Yokoyama,et al. A hybridoma-based in vitro translation system that efficiently synthesizes glycoproteins. , 2006, Journal of biotechnology.
[4] M. Taussig,et al. Enhanced cell‐free protein expression by fusion with immunoglobulin Cκ domain , 2006 .
[5] Y. Gohon,et al. Amphipathic polymers: tools to fold integral membrane proteins to their active form. , 2006, Biochemistry.
[6] Carola Engler,et al. Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors , 2006, Proceedings of the National Academy of Sciences.
[7] R. Burton,et al. Hydrolysis of (1,4)-β-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-β-D-mannan endohydrolase and β-D-mannosidase , 2006 .
[8] N. Dixon. Cell‐free protein synthesis , 2006 .
[9] V. Dötsch,et al. Cell‐free expression as an emerging technique for the large scale production of integral membrane protein , 2006, The FEBS journal.
[10] Samuel Wagner,et al. Rationalizing membrane protein overexpression. , 2006, Trends in biotechnology.
[11] I. Campbell,et al. Cell‐free expression and selective isotope labelling in protein NMR , 2006, Magnetic resonance in chemistry : MRC.
[12] Duncan P. Brown,et al. Functional Classification Using Phylogenomic Inference , 2006, PLoS Comput. Biol..
[13] Shigeyuki Yokoyama,et al. An efficient mammalian cell-free translation system supplemented with translation factors. , 2006, Protein expression and purification.
[14] S. Marillonnet,et al. Protection conferred by recombinant Yersinia pestis antigens produced by a rapid and highly scalable plant expression system. , 2006, Proceedings of the National Academy of Sciences of the United States of America.
[15] Volker Dötsch,et al. Evaluation of detergents for the soluble expression of α‐helical and β‐barrel‐type integral membrane proteins by a preparative scale individual cell‐free expression system , 2005 .
[16] S. Marillonnet,et al. High-yield production of authentic human growth hormone using a plant virus-based expression system. , 2005, Plant biotechnology journal.
[17] Y. Hayashizaki,et al. Crystal structure of an enhancer of rudimentary homolog (ERH) at 2.1 Å resolution , 2005 .
[18] D. Waugh,et al. Making the most of affinity tags. , 2005, Trends in biotechnology.
[19] S. Marillonnet,et al. Systemic Agrobacterium tumefaciens–mediated transfection of viral replicons for efficient transient expression in plants , 2005, Nature Biotechnology.
[20] S. Marillonnet,et al. Magnifection--a new platform for expressing recombinant vaccines in plants. , 2005, Vaccine.
[21] M. Hearn,et al. Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production , 2005, Journal of molecular recognition : JMR.
[22] C. Wilkerson,et al. Expression of cellulose synthase-like (Csl) genes in insect cells reveals that CslA family members encode mannan synthases. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[23] F. Baneyx,et al. Recombinant protein folding and misfolding in Escherichia coli , 2004, Nature Biotechnology.
[24] P. Bastien,et al. Can heterologous gene expression shed (a torch) light on protein function? , 2004, Trends in biotechnology.
[25] Rainer Fischer,et al. Plant cell cultures for the production of recombinant proteins , 2004, Nature Biotechnology.
[26] John L Markley,et al. Cell-free protein production and labeling protocol for NMR-based structural proteomics , 2004, Nature Methods.
[27] A. Spirin. High-throughput cell-free systems for synthesis of functionally active proteins. , 2004, Trends in biotechnology.
[28] S. Govindarajan,et al. Codon bias and heterologous protein expression. , 2004, Trends in biotechnology.
[29] Houhui Song,et al. Expression of a human lactoferrin N-lobe in Nicotiana benthmiana with potato virus X-based agroinfection , 2004, Biotechnology Letters.
[30] S. Marillonnet,et al. In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[31] C. Radauer,et al. Expression of the B subunit of the heat-labile enterotoxin of Escherichia coli in tobacco mosaic virus-infected Nicotiana benthamiana plants and its characterization as mucosal immunogen and adjuvant. , 2004, Journal of immunological methods.
[32] I. Graham,et al. Plant biotechnology: Molecular farming and metabolic engineering promise a new generation of high-tech crops , 2004 .
[33] S. Marillonnet,et al. Engineering viral expression vectors for plants: the 'full virus' and the 'deconstructed virus' strategies. , 2004, Current opinion in plant biology.
[34] R. Reski,et al. The moss bioreactor. , 2004, Current opinion in plant biology.
[35] Rainer Fischer,et al. Plant-based production of biopharmaceuticals. , 2004, Current opinion in plant biology.
[36] V. Gomord,et al. Posttranslational modification of therapeutic proteins in plants. , 2004, Current opinion in plant biology.
[37] I. Raskin,et al. A quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots , 2004, Plant Cell Reports.
[38] T. Sawasaki,et al. Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ. , 2003, European Journal of Biochemistry.
[39] R. Reski,et al. N‐Glycosylation in the Moss Physcomitrella patens is Organized Similarly to that in Higher Plants , 2003 .
[40] Pascal Braun,et al. High throughput protein production for functional proteomics. , 2003, Trends in biotechnology.
[41] D. Rendić,et al. Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris. , 2003, Biochimie.
[42] D. Baulcombe,et al. An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. , 2003, The Plant journal : for cell and molecular biology.
[43] W. Tanner,et al. Specific lipid requirements of membrane proteins--a putative bottleneck in heterologous expression. , 2003, Biochimica et biophysica acta.
[44] Shigeyuki Yokoyama,et al. Protein expression systems for structural genomics and proteomics. , 2003, Current opinion in chemical biology.
[45] G. Lomonossoff,et al. Viruses as Vectors for the Expression of Foreign Sequences in Plants , 2002, Biotechnology & genetic engineering reviews.
[46] Tomio Ogasawara,et al. A cell-free protein synthesis system for high-throughput proteomics , 2002, Proceedings of the National Academy of Sciences of the United States of America.
[47] Ikuo Fujii,et al. Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system , 2002, FEBS letters.
[48] D. Salt,et al. Functional activity and role of cation-efflux family members in Ni hyperaccumulation in Thlaspi goesingense , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[49] R. Perrin,et al. Cellulose: How many cellulose synthases to make a plant? , 2001, Current Biology.
[50] G. Novelli,et al. Transfer and expression of foreign genes in mammalian cells. , 2000, BioTechniques.
[51] W. Tanner,et al. Properties of a reconstituted eukaryotic hexose/proton symporter solubilized by structurally related non-ionic detergents: specific requirement of phosphatidylcholine for permease stability. , 2000, Biochimica et biophysica acta.
[52] Y Endo,et al. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. , 2000, Proceedings of the National Academy of Sciences of the United States of America.
[53] R G Harrison,et al. New fusion protein systems designed to give soluble expression in Escherichia coli. , 1999, Biotechnology and bioengineering.
[54] S. Makrides. Components of vectors for gene transfer and expression in mammalian cells. , 1999, Protein expression and purification.
[55] L. Lyford,et al. Cell-free Expression and Functional Reconstitution of Homo-oligomeric α7 Nicotinic Acetylcholine Receptors into Planar Lipid Bilayers* , 1999, The Journal of Biological Chemistry.
[56] N. Raikhel,et al. Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis. , 1999, Science.
[57] W. Tanner,et al. Expression of eukaryotic plasma membrane transporter HUP1 from Chlorella kessleri in Escherichia coli. , 1999, FEMS microbiology letters.
[58] E. Berger,et al. The yeast expression system for recombinant glycosyltransferases , 1999, Glycoconjugate Journal.
[59] Yasuhiko Yoshida,et al. Cell‐free production and stable‐isotope labeling of milligram quantities of proteins , 1999, FEBS letters.
[60] D. Schaefer,et al. Efficient gene targeting in the moss Physcomitrella patens. , 1997, The Plant journal : for cell and molecular biology.
[61] A. Gronenborn,et al. Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR , 1997, Protein science : a publication of the Protein Society.
[62] L. King,et al. Stable insect cell cultures for recombinant protein production. , 1997, Current opinion in biotechnology.
[63] Kost. Expression systems: Gene expression systems in the genomics era. , 1997, Current opinion in biotechnology.
[64] F. Perler,et al. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. , 1997, Gene.
[65] D. Cove,et al. Mosses as model systems , 1997 .
[66] H P Kocher,et al. Eukaryotic expression systems: a comparison. , 1996, Protein expression and purification.
[67] M. Eckart,et al. Quality and authenticity of heterologous proteins synthesized in yeast. , 1996, Current opinion in biotechnology.
[68] M. Weickert,et al. Optimization of heterologous protein production in Escherichia coli. , 1996, Current opinion in biotechnology.
[69] Olins. Quantity versus authenticity of heterologously produced proteins: an inevitable compromise? , 1996, Current opinion in biotechnology.
[70] C. Kurland,et al. Errors of heterologous protein expression. , 1996, Current opinion in biotechnology.
[71] G. Georgiou,et al. Expression of correctly folded proteins in Escherichia coli. , 1996, Current opinion in biotechnology.
[72] I. Sen,et al. Different Glycosylation Requirements for the Synthesis of Enzymatically Active Angiotensin-converting Enzyme in Mammalian Cells and Yeast (*) , 1996, The Journal of Biological Chemistry.
[73] G. Lorimer,et al. A quantitative assessment of the role of the chaperonin proteins in protein folding in vivo , 1996, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.
[74] C. Tate,et al. Overexpression of integral membrane proteins for structural studies , 1995, Quarterly Reviews of Biophysics.
[75] U. Fischer,et al. Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system. , 1994, The Plant journal : for cell and molecular biology.
[76] R. Wetzel. Mutations and off-pathway aggregation of proteins. , 1994, Trends in biotechnology.
[77] J. Mccoy,et al. A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm , 1993, Bio/Technology.
[78] P. Blum,et al. Physiological consequences of DnaK and DnaJ overproduction in Escherichia coli , 1992, Journal of bacteriology.
[79] K. Ito,et al. Production of an enzymatic active protein using a continuous flow cell-free translation system. , 1992, Journal of biotechnology.
[80] A. Spirin,et al. A continuous cell-free translation system capable of producing polypeptides in high yield. , 1988, Science.
[81] D. Smith,et al. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. , 1988, Gene.
[82] Chu di Guana,et al. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. , 1988 .
[83] Marshall W. Nirenberg,et al. The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides , 1961, Proceedings of the National Academy of Sciences.
[84] Takashi Suzuki,et al. Cell‐Free Protein Synthesis System Prepared from Insect Cells by Freeze‐Thawing , 2006, Biotechnology progress.
[85] Dong-Myung Kim,et al. Cell‐Free Production of Aggregation‐Prone Proteins in Soluble and Active Forms , 2005, Biotechnology progress.
[86] T. Sawasaki,et al. High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system , 2004, Journal of Structural and Functional Genomics.
[87] R. Reski,et al. Physcomitrella patens is highly tolerant against drought, salt and osmotic stress , 2004, Planta.
[88] J. Randles,et al. Host responses to transient expression of individual genes encoded by Tomato leaf curl virus. , 2004, Molecular plant-microbe interactions : MPMI.
[89] Sophie Bonnal,et al. IRESdb: the Internal Ribosome Entry Site database , 2003, Nucleic Acids Res..
[90] V. Gomord,et al. Structural requirements for Arabidopsis beta1,2-xylosyltransferase activity and targeting to the Golgi. , 2003, The Plant journal : for cell and molecular biology.
[91] A. Bellamy,et al. The potential of plant viral vectors and transgenic plants for subunit vaccine production. , 2002, Advances in virus research.
[92] K. Terpe. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems , 2002, Applied Microbiology and Biotechnology.
[93] W G Hol,et al. International Journal for Parasitology 30 (2000) 113±118 Rapid communication , 2000 .
[94] D. Smith. Generating fusions to glutathione S-transferase for protein studies. , 2000, Methods in enzymology.
[95] D. Waugh,et al. Escherichia coli maltose‐binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused , 1999, Protein science : a publication of the Protein Society.
[96] A. Landy. Dynamic, structural, and regulatory aspects of lambda site-specific recombination. , 1989, Annual review of biochemistry.
[97] C Marque,et al. Human abdominal EHG processing for uterine contraction monitoring. , 1989, Biotechnology.
[98] A. Elbein,et al. Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains. , 1987, Annual review of biochemistry.
[99] E. Hochuli,et al. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. , 1987, Journal of chromatography.
[100] G. Zubay,et al. In vitro synthesis of protein in microbial systems. , 1973, Annual review of genetics.