Biotechnology and Bioengineering

Correspondence Aura Ontiveros‐Valencia, School of Sustainable Engineering and the Built Environment, Arizona State University, Biodesign Institute, P.O. Box 875701, Tempe, AZ 85287‐5701. Email: avontive@asu.edu; aura_ontiveros@tec.mx Abstract Chloroform (CF) can undergo reductive dechlorination to dichloromethane, chloromethane, and methane. However, competition for hydrogen (H2), the electron‐donor substrate, may cause poor dechlorination when multiple electron acceptors are present. Common acceptors in anaerobic environments are nitrate (NO3 ), sulfate (SO4 ), and bicarbonate (HCO3 ). We evaluated CF dechlorination in the presence of HCO3 − at 1.56 e Eq/m‐day, then NO3 at 0.04–0.15 e Eq/m‐day, and finally NO3 − (0.04 e Eq/m‐day) along with SO4 at 0.33 e Eq/m‐day in an H2‐ based membrane biofilm reactor (MBfR). When the biofilm was initiated with CF‐ dechlorination conditions (no NO3 − or SO4 ), it yielded a CF flux of 0.14 e Eq/m‐ day and acetate production via homoacetogenesis up to 0.26 e eq/m‐day. Subsequent addition of NO3 − at 0.05 e Eq/m‐day maintained full CF dechlorination and homoacetogenesis, but NO3 − input at 0.15 e Eq/m‐day caused CF to remain in the reactor’s effluent and led to negligible acetate production. The addition of SO4 2−

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