Detection of rabbit and bovine antibodies to the outer membrane of ruminal Bacteroides ruminicola subspecies ruminicola by enzyme-linked immunosorbent assay (ELISA).

The enzyme-linked immunosorbent assay (ELISA) was used to detect specific antibodies in rabbit and bovine sera directed to the outer membrane antigen of a ruminal bacteria, Bacteroides ruminicola subsp. ruminicola. Optimal conditions of ELISA were 10μg of the outer membrane antigen per well and 1:500 dilution of antiserum from rabbit immunized with organisms of B. ruminicola subsp. ruminicola. The ELISA recognized antibody against B. ruminicola subsp. ruminicola subspecies-specifically. Sera from colostrum-deprived calves contained none of specific antibodies, while sera from colostrum-fed calves contained high level of antibodies. The mean titer of sera from 10 day-old calves was significantly (p<0.01) higher than that of 40 day-old calves, but was significantly (p<0.01) lower than that of adult cattle. The mean titer of sera from dairy cows which fed high-roughage diet was significantly (p<0.01) higher than that of feedlot cattle which fed high-concentrate diet. The results suggest that the antibodies against the outer membrane antigen of B. ruminicola subsp. ruminicola transfer to calves via the colostrum, and that the antibody levels of cows are affected by the wav of feed management. Jpn. J. Zootech. Sci., 61 (8): 719-724, 1990

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