Single-beam coherent Raman spectroscopy and microscopy via spectral notch shaping.

We present a simple and easily implementable scheme for multiplexed Coherent Anti-Stokes Raman Scattering (CARS) spectroscopy and microscopy using a single femtosecond pulse, shaped with a narrow spectral notch. We show that a tunable spectral notch, shaped by a resonant photonic crystal slab, can serve as a narrowband, optimally time-delayed probe, resolving a broad vibrational spectrum with high spectral resolution in a single-shot measurement. Our single-source, single-beam scheme allows the simple transformation of any multiphoton microscope with adequate bandwidth into a nearly alignment-free CARS microscope.

[1]  S. Konorov,et al.  Noise autocorrelation spectroscopy with coherent Raman scattering , 2007, 0710.3173.

[2]  A. Friesem,et al.  Resonant grating waveguide structures , 1997 .

[3]  Yaron Silberberg,et al.  Single-pulse coherently controlled nonlinear Raman spectroscopy and microscopy , 2002, Nature.

[4]  M. S. Zubairy,et al.  FAST CARS: Engineering a laser spectroscopic technique for rapid identification of bacterial spores , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[5]  A. Miyawaki,et al.  Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse. , 2009, Optics express.

[6]  Marcus Motzkus,et al.  Single-beam CARS spectroscopy applied to low-wavenumber vibrational modes , 2006 .

[7]  Mischa Bonn,et al.  Direct extraction of Raman line-shapes from congested CARS spectra. , 2006, Optics express.

[8]  Stephen R. Leone,et al.  Fourier transform spectral interferometric coherent anti-Stokes Raman scattering (FTSI-CARS) spectroscopy , 2007 .

[9]  Marcus Motzkus,et al.  Rapid polymer blend imaging with quantitative broadband multiplex CARS microscopy , 2007 .

[10]  M. Cicerone,et al.  Fast extraction of resonant vibrational response from CARS spectra with arbitrary nonresonant background , 2009 .

[11]  A. Weiner.  Femtosecond pulse shaping using spatial light modulators , 2000 .

[12]  Y. Silberberg,et al.  Standoff Detection of Solid Traces by Single-Beam Nonlinear Raman Spectroscopy Using Shaped Femtosecond Pulses , 2008 .

[13]  Thomas Hellerer,et al.  Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses , 2004 .

[14]  Yaron Silberberg,et al.  Single-pulse phase-contrast nonlinear Raman spectroscopy. , 2002, Physical review letters.

[15]  Bi-Chang Chen,et al.  Optimal laser pulse shaping for interferometric multiplex coherent anti-stokes Raman scattering microscopy. , 2008, The journal of physical chemistry. B.

[16]  Marcus Motzkus,et al.  Time-resolving molecular vibration for microanalytics: single laser beam nonlinear Raman spectroscopy in simulation and experiment. , 2008, Physical chemistry chemical physics : PCCP.

[17]  Andreas Volkmer,et al.  Vibrational imaging and microspectroscopies based on coherent anti-Stokes Raman scattering microscopy , 2005 .

[18]  Jane A Dickerson,et al.  Current Applications of Liquid Chromatography / Mass Spectrometry in Pharmaceutical Discovery After a Decade of Innovation , 2008 .

[19]  Andrew Ridsdale,et al.  Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator. , 2009, Optics express.

[20]  Y. Silberberg,et al.  Nonlinear pulse shaping and causality. , 1993, Optics letters.

[21]  Antigoni Alexandrou,et al.  Fourier-transform coherent anti-Stokes Raman scattering microscopy. , 2006, Optics letters.

[22]  Annika Enejder,et al.  Coherent Anti-Stokes Raman Scattering Microscopy , 2012 .

[23]  Marcos Dantus,et al.  Standoff and arms-length detection of chemicals with single-beam coherent anti-Stokes Raman scattering. , 2009, Applied optics.

[24]  Ramón Carriles,et al.  Invited review article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy. , 2009, The Review of scientific instruments.

[25]  Yaron Silberberg,et al.  Single-pulse coherent anti-Stokes Raman spectroscopy in the fingerprint spectral region , 2003 .

[26]  Yaron Silberberg,et al.  Femtosecond phase-and-polarization control for background-free coherent anti-Stokes Raman spectroscopy. , 2003, Physical review letters.

[27]  Dobryna Zalvidea,et al.  Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures. , 2008, Optics express.

[28]  All-optical processing in coherent nonlinear spectroscopy , 2004 .

[29]  U. I. Urbana-Champaign,et al.  Non-linear interferometric vibrational imaging , 2003, Conference on Lasers and Electro-Optics, 2003. CLEO '03..

[30]  Marlan O Scully,et al.  Optimizing the Laser-Pulse Configuration for Coherent Raman Spectroscopy , 2007, Science.

[31]  Wolfgang Werner Langbein,et al.  Single source coherent anti-Stokes Raman microspectroscopy using spectral focusing , 2009 .