Investigating the zoonotic origin of the West African Ebola epidemic

The severe Ebola virus disease epidemic occurring in West Africa stems from a single zoonotic transmission event to a 2‐year‐old boy in Meliandou, Guinea. We investigated the zoonotic origins of the epidemic using wildlife surveys, interviews, and molecular analyses of bat and environmental samples. We found no evidence for a concurrent outbreak in larger wildlife. Exposure to fruit bats is common in the region, but the index case may have been infected by playing in a hollow tree housing a colony of insectivorous free‐tailed bats (Mops condylurus). Bats in this family have previously been discussed as potential sources for Ebola virus outbreaks, and experimental data have shown that this species can survive experimental infection. These analyses expand the range of possible Ebola virus sources to include insectivorous bats and reiterate the importance of broader sampling efforts for understanding Ebola virus ecology.

[1]  Joseph N. Fair,et al.  Undiagnosed Acute Viral Febrile Illnesses, Sierra Leone , 2014, Emerging infectious diseases.

[2]  P Brès,et al.  [The epidemic of Ebola haemorrhagic fever in Sudan and Zaire, 1976: introductory note]. , 1978, Bulletin of the World Health Organization.

[3]  Timothy D. Flietstra,et al.  Seasonal Pulses of Marburg Virus Circulation in Juvenile Rousettus aegyptiacus Bats Coincide with Periods of Increased Risk of Human Infection , 2012, PLoS pathogens.

[4]  T. Ksiazek,et al.  Experimental inoculation of plants and animals with Ebola virus. , 1996, Emerging infectious diseases.

[5]  E. Willerslev,et al.  Blocking human contaminant DNA during PCR allows amplification of rare mammal species from sedimentary ancient DNA , 2012, Molecular ecology.

[6]  P. Formenty,et al.  Multiple Ebola Virus Transmission Events and Rapid Decline of Central African Wildlife , 2004, Science.

[7]  S. Günther,et al.  Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories , 2007, The Journal of infectious diseases.

[8]  C. Boesch,et al.  Absence of evidence is not evidence of absence: Discovery of a large, continuous population of Pan troglodytes schweinfurthii in the Central Uele region of northern DRC , 2014 .

[9]  Stephan Günther,et al.  Emergence of Zaire Ebola virus disease in Guinea. , 2014, The New England journal of medicine.

[10]  Kevin J. Olival,et al.  Filoviruses in Bats: Current Knowledge and Future Directions , 2014, Viruses.

[11]  J. Gonzalez,et al.  Fruit bats as reservoirs of Ebola virus , 2005, Nature.

[12]  E. Tkachenko,et al.  [Clinico-epidemiologic and laboratory research on hemorrhagic fevers in Guinea]. , 1987, Bulletin de la Societe de pathologie exotique et de ses filiales.

[13]  J. Gonzalez,et al.  Large serological survey showing cocirculation of Ebola and Marburg viruses in Gabonese bat populations, and a high seroprevalence of both viruses in Rousettus aegyptiacus , 2009, BMC infectious diseases.

[14]  Bas Huijbregts,et al.  Catastrophic ape decline in western equatorial Africa , 2003, Nature.

[15]  A. Schuh,et al.  Marburgvirus Resurgence in Kitaka Mine Bat Population after Extermination Attempts, Uganda , 2014, Emerging infectious diseases.

[16]  A. Sanchez,et al.  The reemergence of Ebola hemorrhagic fever, Democratic Republic of the Congo, 1995. Commission de Lutte contre les Epidémies à Kikwit. , 1999, The Journal of infectious diseases.

[17]  P. Racey,et al.  Bats as bushmeat: a global review , 2009, Oryx.

[18]  M. Georges-Courbot,et al.  Ebola hemorrhagic fever outbreaks in Gabon, 1994-1997: epidemiologic and health control issues. , 1999, The Journal of infectious diseases.

[19]  B. Renard,et al.  Clock Rooting Further Demonstrates that Guinea 2014 EBOV is a Member of the Zaïre Lineage , 2014, PLoS currents.

[20]  Rachel S. G. Sealfon,et al.  Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak , 2014, Science.

[21]  A. Rambaut,et al.  Phylogenetic Analysis of Guinea 2014 EBOV Ebolavirus Outbreak , 2014, PLoS currents.

[22]  Eric R. Ziegel,et al.  Generalized Linear Models , 2002, Technometrics.

[23]  David L. Borchers,et al.  Distance sampling , 2001 .

[24]  P. G. Taylor Reproducibility of ancient DNA sequences from extinct Pleistocene fauna. , 1996, Molecular biology and evolution.

[25]  Christophe Boesch,et al.  Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity. , 2013, Molecular ecology.