Glycosylation of Acetylcholinesterase Forms in Microsomal Membranes from Normal and Dystrophic Lama2dy Mouse Muscle

Abstract: The distribution and glycosylation of acetylcholinesterase (AChE) forms in vesicles derived from sarcoplasmic reticulum of normal muscle (NMV) were investigated and compared with those from dystrophic muscle vesicles (DMV). AChE activity was similar in NMV and DMV. Most of the AChE in NMV and half in DMV were released with Triton X‐100. Asymmetric (A12) and globular hydrophilic and amphiphilic (GH4, GA4, GA2, and GA1) AChE species occurred in NMV and DMV, the lighter forms being predominant. The percentage of GH4 and GA4 decreased in DMV. A fraction of the AChE that could not be extracted with detergent was detached with collagenase. Most of the detergent‐released A12 AChE from NMV and nearly half in DMV failed to bind to Ricinus communis agglutinin (RCA‐I). Conversely, the collagenase‐detached isoforms bound to RCA, revealing that asymmetric AChE associated with internal membranes or basal lamina differed in glycosylation. Moreover, nearly half of GA4 AChE in DMV and a few in NMV bound to RCA. Most of the RCA‐unreactive GA4 forms in NMV come from sarcolemma. The results indicate that dystrophy induces minor changes in the distribution and glycosylation of AChE forms in internal membranes of muscle.

[1]  F. Campoy,et al.  Glycosylation of cholinesterase forms in brain from normal and dystrophic Lama2dy mice , 1997, Neuroscience Letters.

[2]  M. E. Kargacin,et al.  The sarcoplasmic reticulum calcium pump is functionally altered in dystrophic muscle. , 1996, Biochimica et biophysica acta.

[3]  N. Inestrosa,et al.  Tetrameric (G4) Acetylcholinesterase: Structure, Localization, and Physiological Regulation , 1996, Journal of neurochemistry.

[4]  C. J. Vidal,et al.  Molecular forms of acetyl‐ and butyrylcholinesterase in normal and dystrophic mouse brain , 1996, Journal of neuroscience research.

[5]  M. J. Dauncey,et al.  Ouabain sensitive Na+ K+-ATPase content is elevated in mdx mice: Implications for the regulation of ions in dystrophic muscle , 1995, Journal of the Neurological Sciences.

[6]  S. Noguchi,et al.  Dystrophin-associated proteins in muscular dystrophy. , 1995, Human molecular genetics.

[7]  K. Campbell.  Three muscular dystrophies: Loss of cytoskeleton-extracellular matrix linkage , 1995, Cell.

[8]  F. Campoy,et al.  Amphiphilic properties of molecular forms of acetylcholinesterase in normal and dystrophic muscle , 1994, Journal of neuroscience research.

[9]  K. Campbell,et al.  Deficiency of merosin in dystrophic dy mice and genetic linkage of laminin M chain gene to dy locus. , 1994, The Journal of biological chemistry.

[10]  B. No̸rgaard-Pedersen,et al.  Overexpressed Monomeric Human Acetylcholinesterase Induces Subtle Ultrastructural Modifications in Developing Neuromuscular Junctions of Xenopus laevis Embryos , 1994, Journal of neurochemistry.

[11]  K. Campbell,et al.  Congenital muscular dystrophy with merosin deficiency. , 1994, Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie.

[12]  F. Campoy,et al.  G4 forms of acetylcholinesterase and butyrylcholinesterase in normal and dystrophic mouse muscle differ in their interaction with Ricinus communis agglutinin. , 1994, Biochimica et biophysica acta.

[13]  P Taylor,et al.  The cholinesterases: from genes to proteins. , 1994, Annual review of pharmacology and toxicology.

[14]  E. Schweitzer.  Regulated and constitutive secretion of distinct molecular forms of acetylcholinesterase from PC12 cells. , 1993, Journal of cell science.

[15]  R. Rotundo,et al.  Localization of "non-extractable" acetylcholinesterase to the vertebrate neuromuscular junction. , 1993, The Journal of biological chemistry.

[16]  F. Vallette,et al.  Molecular and cellular biology of cholinesterases , 1993, Progress in Neurobiology.

[17]  F. Campoy,et al.  Interaction of AChE with Lens culinaris agglutinin reveals differences in glycosylation of molecular forms in sarcoplasmic reticulum membrane subfractions , 1992, Journal of neuroscience research.

[18]  J. Tidball,et al.  Calpain concentration is elevated although net calcium-dependent proteolysis is suppressed in dystrophin-deficient muscle. , 1992, Experimental cell research.

[19]  T. Arendt,et al.  Changes in acetylcholinesterase and butyrylcholinesterase in Alzheimer's disease resemble embryonic development—A study of molecular forms , 1992, Neurochemistry International.

[20]  C. J. Vidal,et al.  Ricinus communis agglutinin I reacting and non-reacting butyrylcholinesterase in human cerebrospinal fluid , 1992, Neuroscience Letters.

[21]  E. Muñoz-Delgado,et al.  Alkaline treatment of muscle microsomes releases amphiphilic and hydrophilic forms of acetylcholinesterase. , 1992, Biochimica et biophysica acta.

[22]  E. Engvall,et al.  Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[23]  K. Skau.  On the specificity of the acetylcholinesterase defect in dystrophic mice , 1990, Muscle & nerve.

[24]  A. Megías,et al.  Abnormal properties of Mg2(+)-ATPase in transverse tubule membranes from dystrophic chicken. , 1990, Archives of biochemistry and biophysics.

[25]  S. Rummel,et al.  Coated vesicles from developing and adult rat skeletal muscles contain multiple molecular forms of acetylcholinesterase , 1989, Journal of neuroscience research.

[26]  R. Fine,et al.  Intracellular transport, sorting, and turnover of acetylcholinesterase. Evidence for an endoglycosidase H-sensitive form in Golgi apparatus, sarcoplasmic reticulum, and clathrin-coated vesicles and its rapid degradation by a non-lysosomal mechanism. , 1989, The Journal of biological chemistry.

[27]  K. Campbell,et al.  Newly synthesized calsequestrin, destined for the sarcoplasmic reticulum, is contained in early/intermediate Golgi-derived clathrin-coated vesicles. , 1989, The Journal of biological chemistry.

[28]  L. Kunkel,et al.  Dystrophin abnormalities in Duchenne/Becker muscular dystrophy , 1989, Neuron.

[29]  R. Rotundo.  Biogenesis of acetylcholinesterase molecular forms in muscle. Evidence for a rapidly turning over, catalytically inactive precursor pool. , 1988, The Journal of biological chemistry.

[30]  R. Steinhardt,et al.  Increased protein degradation results from elevated free calcium levels found in muscle from mdx mice , 1988, Nature.

[31]  V. Gisiger,et al.  Localization of the pool of G4 acetylcholinesterase characterizing fast muscles and its alteration in murine muscular dystrophy , 1988, Journal of neuroscience research.

[32]  E. Muñoz-Delgado,et al.  Acetylcholinesterase in membrane fractions derived from sarcotubular system of skeletal muscle: presence of monomeric acetylcholinesterase in sarcoplasmic reticulum and transverse tubule membranes , 1987, Neurochemistry International.

[33]  S. Fleischer,et al.  Preparation and characterization of longitudinal tubules of sarcoplasmic reticulum from fast skeletal muscle. , 1987, Archives of biochemistry and biophysics.

[34]  T. Clausen.  Regulation of active Na+-K+ transport in skeletal muscle. , 1986, Physiological reviews.

[35]  J. Mickelson,et al.  Components of purified sarcolemma from porcine skeletal muscle. , 1985, Archives of biochemistry and biophysics.

[36]  A. Wareham,et al.  [3H]Ouabain binding in normal and dystrophic mouse skeletal muscles and the effect of age , 1985, Journal of the Neurological Sciences.

[37]  R. Rotundo.  Asymmetric acetylcholinesterase is assembled in the Golgi apparatus. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[38]  J. Mendell,et al.  RELEVANCE OF GENETIC ANIMAL MODELS OF MUSCULAR DYSTROPHY TO HUMAN MUSCULAR DYSTROPHIES * , 1979, Annals of the New York Academy of Sciences.

[39]  J. Sanes,et al.  Cholinesterase is associated with the basal lamina at the neuromuscular junction , 1978, Nature.

[40]  A. Caswell,et al.  Isolation of transverse tubules by fractionation of triad junctions of skeletal muscle. , 1977, The Journal of biological chemistry.

[41]  W. Bradley,et al.  Abnormalities of peripheral nerves in murine muscular dystrophy. , 1973, Journal of the neurological sciences.