Biochemical and genetic heterogeneity of staphylococcal protein A.

We investigated the biochemical and genetic heterogeneity of protein A from Staphylococcus aureus. SpA genes (spas) of various strains were heterogeneous when detected as DraI and EcoRV fragments of chromosomal DNA. Polymerase chain reaction using primers to detect DNA encoding the IgG-binding domains in spa revealed that they numbered between 2 and 5. Protein A from several S. aureus strains showed two types of reactivities to immunoglobulins in normal canine serum according to the number of active domains.

[1]  A. Cheung,et al.  Analysis by gel electrophoresis, Western blot, and peptide mapping of protein A heterogeneity in Staphylococcus aureus strains , 1987, Infection and immunity.

[2]  M. Uhlén,et al.  Analysis of protein A encoded by a mutated gene of Staphylococcus aureus Cowan I. , 1985, European journal of biochemistry.

[3]  B Guss,et al.  Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications. , 1984, The Journal of biological chemistry.

[4]  S. Masuda,et al.  Mechanism of the Spur Formation Observed between Two Forms of Extracellular Staphylococcal Protein A Produced by Mutants against Normal Canine Serum , 1981, Microbiology and immunology.

[5]  J. Sjöquist,et al.  Physico‐ and Immunochemical Properties of Staphylococcal Protein A Extracellularly Produced by a Set of Mutants from Staphylococcus aureus Cowan I , 1979, Microbiology and immunology.

[6]  J. Sjödahl,et al.  Structural studies on the four repetitive Fc-binding regions in protein A from Staphylococcus aureus. , 1977, European journal of biochemistry.

[7]  S. Sakurai,et al.  Simple and effective method for selecting protein A-dificient mutants by consedimentation with sensitized sheep erythrocytes , 1975, Infection and immunity.

[8]  R. Brentani,et al.  Nucleotide sequence of a variant protein A of Staphylococcus aureus suggests molecular heterogeneity among strains , 1991, Journal of basic microbiology.

[9]  C. J. Duggleby,et al.  Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli. , 1987, Gene.